gamma Catenin (JUP) (NM_002230) Human Tagged ORF Clone

CAT#: RC204209

JUP (Myc-DDK-tagged)-Human junction plakoglobin (JUP), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_002230" in other vectors (5)

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CNY 5,456.00

CNY 5,800.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


JUP mouse monoclonal antibody,clone OTI4D5
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CTNNG; DP3; DPIII; PDGB; PG; PKGB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204209 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGATGAACCTGATGGAGCAGCCTATCAAGGTGACTGAGTGGCAGCAGACATACACCTACGACT
CGGGTATCCACTCGGGCGCCAACACCTGCGTGCCCTCCGTCAGCAGCAAGGGCATCATGGAGGAGGATGA
GGCCTGCGGGCGCCAGTACACGCTCAAGAAAACCACCACTTACACCCAGGGGGTGCCCCCCAGCCAAGGT
GACCTGGAGTACCAGATGTCCACAACAGCCAGGGCCAAACGGGTGCGGGAGGCCATGTGCCCTGGTGTGT
CAGGCGAGGACAGCTCGCTTCTGCTGGCCACCCAGGTGGAGGGGCAGGCCACCAACCTGCAGCGACTGGC
CGAGCCGTCCCAGCTGCTCAAGTCGGCCATTGTGCATCTCATCAACTACCAGGATGATGCCGAGCTGGCC
ACTCGCGCCCTGCCCGAGCTCACCAAACTGCTCAACGACGAGGACCCGGTGGTGGTGACCAAGGCGGCCA
TGATTGTGAACCAGCTGTCGAAGAAGGAGGCGTCGCGGCGGGCCCTGATGGGCTCGCCCCAGCTGGTGGC
CGCTGTCGTGCGTACCATGCAGAATACCAGCGACCTGGACACAGCCCGCTGCACCACCAGCATCCTGCAC
AACCTCTCCCACCACCGGGAGGGGCTGCTCGCCATCTTCAAGTCGGGTGGCATCCCTGCTCTGGTCCGCA
TGCTCAGCTCCCCTGTGGAGTCGGTCCTGTTCTATGCCATCACCACGCTGCACAACCTGCTCCTGTACCA
GGAGGGCGCCAAGATGGCCGTGCGCCTGGCCGACGGGCTGCAAAAGATGGTGCCCCTGCTCAACAAGAAC
AACCCCAAGTTCCTGGCCATCACCACCGACTGCCTGCAGCTCCTGGCCTACGGCAACCAGGAGAGCAAGC
TGATCATCCTGGCCAATGGTGGGCCCCAGGCCCTCGTGCAGATCATGCGTAACTACAGTTATGAAAAGCT
GCTCTGGACCACCAGTCGTGTGCTCAAGGTGCTATCCGTGTGTCCCAGCAATAAGCCTGCCATTGTGGAG
GCTGGTGGGATGCAGGCCCTGGGCAAGCACCTGACCAGCAACAGCCCCCGCCTGGTGCAGAACTGCCTGT
GGACCCTGCGCAACCTCTCAGATGTGGCCACCAAGCAGGAGGGCCTGGAGAGTGTGCTGAAGATTCTGGT
GAATCAGCTGAGTGTGGATGACGTCAACGTCCTCACCTGTGCCACGGGCACACTCTCCAACCTGACATGC
AACAACAGCAAGAACAAGACGCTGGTGACACAGAACAGCGGTGTGGAGGCTCTCATCCATGCCATCCTGC
GTGCTGGTGACAAGGACGACATCACGGAGCCTGCCGTCTGCGCTCTGCGCCACCTCACTAGCCGCCACCC
TGAGGCCGAGATGGCCCAGAACTCTGTGCGTCTCAACTATGGCATCCCAGCCATCGTGAAGCTGCTCAAC
CAGCCCAACCAGTGGCCACTGGTCAAGGCAACCATCGGCTTGATCAGGAATCTGGCCCTGTGCCCAGCCA
ACCATGCCCCGCTGCAGGAGGCAGCGGTCATCCCCCGCCTCGTCCAACTGCTGGTGAAGGCCCACCAGGA
TGCCCAGCGCCACGTAGCTGCAGGCACACAGCAGCCCTACACGGATGGTGTGAGGATGGAGGAGATTGTG
GAGGGCTGCACCGGAGCACTGCACATCCTCGCCCGGGACCCCATGAACCGCATGGAGATCTTCCGGCTCA
ACACCATTCCCCTGTTTGTGCAGCTCCTGTACTCGTCGGTGGAGAACATCCAGCGCGTGGCTGCCGGGGT
GCTGTGTGAGCTGGCCCAGGACAAGGAGGCGGCCGACGCCATTGATGCAGAGGGGGCCTCGGCCCCACTC
ATGGAGTTGCTGCACTCCCGCAACGAGGGCACTGCCACCTACGCTGCTGCCGTCCTGTTCCGCATCTCCG
AGGACAAGAACCCAGACTACCGGAAGCGCGTGTCCGTGGAGCTCACCAACTCCCTCTTCAAGCATGACCC
GGCTGCCTGGGAGGCTGCCCAGAGCATGATTCCCATCAATGAGCCCTATGGAGATGACTTGGATGCCACC
TACCGCCCCATGTACTCCAGCGATGTGCCCCTTGACCCGCTGGAGATGCACATGGACATGGATGGAGACT
ACCCCATCGACACCTACAGCGACGGCCTCAGGCCCCCGTACCCCACTGCAGACCACATGCTGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204209 protein sequence
Red=Cloning site Green=Tags(s)

MEVMNLMEQPIKVTEWQQTYTYDSGIHSGANTCVPSVSSKGIMEEDEACGRQYTLKKTTTYTQGVPPSQG
DLEYQMSTTARAKRVREAMCPGVSGEDSSLLLATQVEGQATNLQRLAEPSQLLKSAIVHLINYQDDAELA
TRALPELTKLLNDEDPVVVTKAAMIVNQLSKKEASRRALMGSPQLVAAVVRTMQNTSDLDTARCTTSILH
NLSHHREGLLAIFKSGGIPALVRMLSSPVESVLFYAITTLHNLLLYQEGAKMAVRLADGLQKMVPLLNKN
NPKFLAITTDCLQLLAYGNQESKLIILANGGPQALVQIMRNYSYEKLLWTTSRVLKVLSVCPSNKPAIVE
AGGMQALGKHLTSNSPRLVQNCLWTLRNLSDVATKQEGLESVLKILVNQLSVDDVNVLTCATGTLSNLTC
NNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNYGIPAIVKLLN
QPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQRHVAAGTQQPYTDGVRMEEIV
EGCTGALHILARDPMNRMEIFRLNTIPLFVQLLYSSVENIQRVAAGVLCELAQDKEAADAIDAEGASAPL
MELLHSRNEGTATYAAAVLFRISEDKNPDYRKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYGDDLDAT
YRPMYSSDVPLDPLEMHMDMDGDYPIDTYSDGLRPPYPTADHMLA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002230
ORF Size 2235 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002230.4
RefSeq Size 3508 bp
RefSeq ORF 2238 bp
Locus ID 3728
UniProt ID P14923
Domains Armadillo_seg
Protein Families Druggable Genome
Protein Pathways Acute myeloid leukemia, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Pathways in cancer
MW 81.7 kDa
Gene Summary This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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