PACSIN3 (NM_016223) Human Tagged ORF Clone

CAT#: RC204103

PACSIN3 (Myc-DDK-tagged)-Human protein kinase C and casein kinase substrate in neurons 3 (PACSIN3), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_016223" in other vectors (5)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


PACSIN3 mouse monoclonal antibody, clone OTI2E4 (formerly 2E4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms SDPIII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204103 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCAGAAGAGGACGCTGGAGGGGAGGCCTTAGGGGGCAGTTTCTGGGAGGCTGGCAACTACAGGC
GCACGGTACAGCGGGTGGAGGACGGGCACCGGCTGTGCGGGGACCTGGTCAGCTGCTTCCAGGAGCGCGC
CCGCATCGAGAAGGCTTATGCCCAGCAGTTGGCTGACTGGGCCCGAAAGTGGAGGGGGACCGTGGAGAAG
GGCCCCCAGTATGGCACACTGGAGAAGGCCTGGCATGCCTTTTTCACGGCGGCTGAGCGGCTGAGCGCGC
TGCACCTGGAGGTGCGGGAGAAGCTGCAAGGGCAGGACAGTGAGCGGGTGCGCGCCTGGCAGCGGGGGGC
TTTCCACCGGCCTGTGCTGGGCGGCTTCCGCGAGAGCCGGGCGGCCGAGGACGGCTTCCGCAAGGCCCAG
AAGCCCTGGCTGAAGAGGCTGAAGGAGGTTGAGGCTTCCAAGAAAAGCTACCACGCAGCCCGGAAGGATG
AGAAGACCGCCCAGACGAGGGAGAGCCACGCAAAGGCAGACAGCGCCGTCTCCCAGGAGCAGCTGCGCAA
ACTGCAGGAACGGGTGGAACGCTGTGCCAAGGAGGCCGAGAAGACAAAAGCTCAGTATGAGCAGACGCTG
GCAGAGCTGCATCGCTACACTCCACGCTACATGGAGGACATGGAACAGGCCTTTGAGACCTGCCAGGCCG
CCGAGCGCCAGCGGCTTCTTTTCTTCAAGGATATGCTGCTCACCTTACACCAGCACCTGGACCTTTCCAG
CAGTGAGAAGTTCCATGAACTCCACCGTGACTTGCACCAGGGCATTGAGGCAGCCAGTGACGAAGAGGAT
CTGCGCTGGTGGCGCAGCACCCACGGGCCAGGCATGGCCATGAACTGGCCACAGTTCGAGGAGTGGTCCT
TGGACACACAGAGGACAATCAGCCGGAAAGAGAAGGGTGGCCGGAGCCCTGATGAGGTTACCCTGACCAG
CATTGTGCCTACAAGAGATGGCACCGCACCCCCACCCCAGTCCCCGGGGTCCCCAGGCACGGGGCAGGAT
GAGGAGTGGTCAGATGAAGAGAGTCCCCGGAAGGCTGCCACCGGGGTTCGGGTGAGGGCACTCTATGACT
ACGCTGGCCAGGAAGCTGATGAGCTGAGCTTCCGAGCAGGGGAGGAGCTGCTGAAGATGAGTGAGGAGGA
CGAGCAGGGCTGGTGCCAAGGCCAGTTGCAGAGTGGCCGCATTGGCCTGTACCCTGCCAACTACGTGGAG
TGTGTGGGCGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204103 protein sequence
Red=Cloning site Green=Tags(s)

MAPEEDAGGEALGGSFWEAGNYRRTVQRVEDGHRLCGDLVSCFQERARIEKAYAQQLADWARKWRGTVEK
GPQYGTLEKAWHAFFTAAERLSALHLEVREKLQGQDSERVRAWQRGAFHRPVLGGFRESRAAEDGFRKAQ
KPWLKRLKEVEASKKSYHAARKDEKTAQTRESHAKADSAVSQEQLRKLQERVERCAKEAEKTKAQYEQTL
AELHRYTPRYMEDMEQAFETCQAAERQRLLFFKDMLLTLHQHLDLSSSEKFHELHRDLHQGIEAASDEED
LRWWRSTHGPGMAMNWPQFEEWSLDTQRTISRKEKGGRSPDEVTLTSIVPTRDGTAPPPQSPGSPGTGQD
EEWSDEESPRKAATGVRVRALYDYAGQEADELSFRAGEELLKMSEEDEQGWCQGQLQSGRIGLYPANYVE
CVGA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016223
ORF Size 1272 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_016223.5
RefSeq Size 1917 bp
RefSeq ORF 1275 bp
Locus ID 29763
UniProt ID Q9UKS6
Domains FCH, SH3
Protein Families Druggable Genome
MW 48.5 kDa
Gene Summary This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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