alpha Tubulin (TUBA3C) (NM_079836) Human Tagged ORF Clone

CAT#: RC204072

TUBA3C (Myc-DDK-tagged)-Human tubulin, alpha 3c (TUBA3C), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_079836" in other vectors (4)

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CNY 3,656.00

CNY 4,180.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Chicken polyclonal anti-TUBA1A(alpha Tubulin) antibody, Loading control
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms bA408E5.3; TUBA2; tubulin, alpha 2; tubulin, alpha 3c
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204072 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTGAGTGTATCTCTATCCACGTGGGGCAGGCAGGAGTCCAGATCGGCAATGCCTGCTGGGAACTGT
ACTGCCTGGAACATGGAATTCAGCCCGATGGTCAGATGCCAAGTGATAAAACCATTGGTGGTGGGGACGA
CTCCTTCAACACGTTCTTCAGTGAGACTGGAGCTGGCAAGCACGTGCCCAGAGCAGTGTTTGTGGACCTG
GAGCCCACTGTGGTCGATGAAGTGCGCACAGGAACCTATAGGCAGCTCTTCCACCCAGAGCAGCTGATCA
CCGGGAAGGAAGATGCGGCCAATAATTACGCCAGAGGCCATTACACCATCGGCAAGGAGATCGTCGACCT
GGTCCTGGACCGGATCCGCAAACTGGCGGATCTGTGCACGGGACTGCAGGGCTTCCTCATCTTCCACAGT
TTTGGGGGTGGCACTGGCTCTGGGTTCGCATCTCTGCTCATGGAGCGGCTCTCAGTGGATTACGGCAAGA
AGTCCAAGCTAGAATTTGCCATTTACCCAGCCCCCCAGGTCTCCACGGCCGTGGTGGAGCCCTACAACTC
CATCCTGACCACCCACACGACCCTGGAACATTCTGACTGTGCCTTCATGGTCGACAATGAAGCCATCTAT
GACATATGTCGGCGCAACCTGGACATCGAGCGTCCCACGTACACCAACCTCAATCGCCTGATTGGGCAGA
TCGTGTCCTCCATCACGGCCTCCCTGCGATTTGACGGGGCCCTGAATGTGGACTTGACGGAATTCCAGAC
CAACCTAGTGCCGTACCCCCGCATCCACTTCCCCCTGGCCACCTACGCCCCGGTCATCTCAGCCGAGAAG
GCCTACCACGAGCAGCTGTCCGTGGCTGAGATCACCAATGCCTGCTTCGAGCCAGCCAATCAGATGGTCA
AGTGTGACCCTCGCCACGGCAAGTACATGGCCTGCTGCATGTTGTACAGGGGGGATGTGGTCCCGAAAGA
TGTCAACGCGGCCATCGCCACCATCAAGACCAAGCGCACCATCCAGTTTGTAGATTGGTGCCCAACTGGA
TTTAAGGTGGGCATTAACTACCAGCCCCCCACGGTGGTCCCTGGGGGAGACCTGGCCAAGGTGCAGCGGG
CTGTGTGCATGCTGAGCAACACCACGGCCATCGCGGAGGCCTGGGCTCGCCTGGACCTGGCAGCTCTGGA
GAAGGATTATGAAGAGGTGGGCGTGGATTCCGTGGAAGCCGAGGCTGAAGAAGGTGAAGAATAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204072 protein sequence
Red=Cloning site Green=Tags(s)

MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL
EPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHS
FGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY
DICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK
AYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTG
FKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDLAALEKDYEEVGVDSVEAEAEEGEEY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_079836
ORF Size 1254 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_079836.1, NP_524575.1
RefSeq Size 1408 bp
RefSeq ORF 1256 bp
Locus ID 7278
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Gap junction, Pathogenic Escherichia coli infection
MW 46.1 kDa
Gene Summary Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene is an alpha tubulin gene that encodes a protein 99% identical to the mouse testis-specific Tuba3 and Tuba7 gene products. This gene is located in the 13q11 region, which is associated with the genetic diseases Clouston hidrotic ectodermal dysplasia and Kabuki syndrome. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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