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NOSIP (NM_015953) Human Tagged ORF Clone
CAT＃: RC204024
NOSIP (Myc-DDK-tagged)-Human nitric oxide synthase interacting protein (NOSIP)
ORF Plasmid: tGFP
"NM_015953" in other vectors (5)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGI-25 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204024 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCGGCATGGCAAGAACTGCACCGCAGGGGCCGTCTACACCTACCACGAGAAGAAGAAGGACACAG CGGCCTCGGGCTATGGGACCCAGAACATTCGACTGAGCCGGGATGCCGTGAAGGACTTCGACTGCTGTTG TCTCTCCCTGCAGCCTTGCCACGATCCTGTTGTCACCCCAGATGGCTACCTGTATGAGCGTGAGGCCATC CTGGAGTACATTCTGCACCAGAAGAAGGAGATTGCCCGGCAGATGAAGGCCTACGAGAAGCAGCGGGGCA CCCGGCGCGAGGAGCAGAAGGAGCTTCAGCGGGCGGCCTCGCAGGACCATGTGCGGGGCTTCCTGGAGAA GGAGTCGGCTATCGTGAGCCGGCCCCTCAACCCTTTCACAGCCAAGGCCCTCTCGGGCACCAGCCCAGAT GATGTCCAACCTGGGCCCAGTGTGGGTCCTCCAAGTAAGGACAAGGACAAAGTGCTGCCCAGCTTCTGGA TCCCGTCGCTGACGCCCGAAGCCAAGGCCACCAAGCTGGAGAAGCCGTCCCGCACGGTGACCTGCCCCAT GTCAGGGAAGCCCCTGCGCATGTCGGACCTGACGCCCGTGCACTTCACACCGCTAGACAGCTCCGTGGAC CGCGTGGGGCTCATCACCCGCAGCGAGCGCTACGTGTGTGCCGTGACCCGCGACAGCCTGAGCAACGCCA CCCCCTGCGCTGTGCTGCGGCCCTCTGGGGCTGTGGTCACCCTCGAATGCGTGGAGAAGCTGATTCGGAA GGACATGGTGGACCCTGTGACTGGAGACAAACTCACAGACCGCGACATCATCGTGCTGCAGCGGGGCGGT ACCGGCTTCGCGGGCTCCGGAGTGAAGCTGCAAGCGGAGAAATCACGGCCGGTGATGCAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204024 protein sequence
Red=Cloning site Green=Tags(s) MTRHGKNCTAGAVYTYHEKKKDTAASGYGTQNIRLSRDAVKDFDCCCLSLQPCHDPVVTPDGYLYEREAI LEYILHQKKEIARQMKAYEKQRGTRREEQKELQRAASQDHVRGFLEKESAIVSRPLNPFTAKALSGTSPD DVQPGPSVGPPSKDKDKVLPSFWIPSLTPEAKATKLEKPSRTVTCPMSGKPLRMSDLTPVHFTPLDSSVD RVGLITRSERYVCAVTRDSLSNATPCAVLRPSGAVVTLECVEKLIRKDMVDPVTGDKLTDRDIIVLQRGG TGFAGSGVKLQAEKSRPVMQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015953 |
| ORF Size | 903 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015953.4 |
| RefSeq Size | 1367 bp |
| RefSeq ORF | 906 bp |
| Locus ID | 51070 |
| UniProt ID | Q9Y314 |
| MW | 33.2 kDa |
| Gene Summary | The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204024L3 | Lenti ORF clone of Human nitric oxide synthase interacting protein (NOSIP), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204024L4 | Lenti ORF clone of Human nitric oxide synthase interacting protein (NOSIP), mGFP tagged |
CNY 5,890.00 |
|
| RG204024 | NOSIP (tGFP-tagged) - Human nitric oxide synthase interacting protein (NOSIP) |
CNY 4,000.00 |
|
| SC108090 | NOSIP (untagged)-Human nitric oxide synthase interacting protein (NOSIP) |
CNY 2,400.00 |
|
| SC320872 | NOSIP (untagged)-Human nitric oxide synthase interacting protein (NOSIP) |
CNY 2,400.00 |
