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PLEKHA4 (NM_020904) Human Tagged ORF Clone
CAT＃: RC203633
PLEKHA4 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4 (PLEKHA4), transcript variant 1
ORF Plasmid: tGFP
"NM_020904" in other vectors (4)
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CNY 5,704.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PEPP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203633 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGAGCCGACCTCGCAGCAGCCTGAGCCTGGCCAGCAGCGCCTCCACCATCTCCTCGCTCAGCA GCCTGAGCCCCAAGAAGCCCACCCGGGCAGTAAACAAGATCCACGCCTTTGGGAAGAGAGGCAATGCGCT CAGGAGGGATCCCAACCTTCCCGTGCACATCCGAGGCTGGCTTCATAAGCAGGACAGCTCGGGGCTCCGT CTCTGGAAACGCCGCTGGTTCGTCCTCTCCGGCCATTGCCTCTTTTATTACAAGGACAGCCGCGAGGAGA GTGTCCTAGGCAGCGTCCTGCTCCCCAGCTACAATATTAGACCAGATGGGCCGGGAGCCCCCCGAGGGCG GCGCTTCACCTTCACCGCAGAGCACCCGGGCATGAGGACCTACGTTTTGGCCGCTGACACCTTAGAAGAC CTGCGGGGCTGGCTACGGGCGCTGGGCCGGGCCTCCCGTGCGGAGGGGGACGACTATGGGCAACCCAGGT CACCTGCACGACCCCAGCCCGGGGAGGGCCCCGGCGGCCCCGGTGGTCCCCCGGAGGTGAGCAGAGGGGA AGAGGGGCGCATCTCAGAATCACCGGAAGTGACTCGACTCTCCAGAGGTCGTGGTAGACCCAGGCTGCTC ACTCCCAGCCCCACAACCGACCTCCACTCTGGACTCCAGATGCGGAGGGCGAGGAGCCCCGACCTGTTCA CCCCCCTCTCTCGCCCTCCCTCGCCTCTGAGCCTCCCCCGTCCCCGTTCTGCCCCTGCGCGGCGACCCCC TGCCCCCTCAGGAGACACAGCACCCCCTGCCCGACCTCACACCCCGTTGAGTCGCATTGATGTCCGACCT CCTCTGGATTGGGGCCCCCAACGCCAGACCCTCTCCCGACCCCCTACTCCCCGCCGAGGACCTCCCTCTG AGGCTGGGGGAGGAAAGCCCCCCAGGAGTCCCCAGCACTGGAGTCAGGAGCCCAGAACACAGGCACACTC TGGCTCCCCCACTTATCTCCAGCTCCCCCCGCGGCCCCCTGGGACCCGGGCCTCCATGGTTTTATTGCCG GGTCCTCCCCTGGAGTCAACTTTCCACCAAAGCTTGGAGACAGATACGCTGCTGACCAAGTTGTGCGGGC AGGACCGGCTTCTGCGGAGGCTGCAGGAGGAGATAGACCAGAAGCAGGAGGAGAAGGAGCAACTAGAAGC AGCTCTGGAGTTGACCCGGCAACAGCTGGGCCAAGCCACCAGGGAGGCTGGGGCTCCCGGGAGGGCCTGG GGTCGCCAGCGCCTCTTGCAGGACCGGCTGGTCAGTGTGAGGGCCACCCTCTGTCACTTGACTCAGGAGC GAGAGAGGGTTTGGGACACGTACAGTGGCCTGGAGCAGGAGCTGGGCACCTTAAGAGAGACGCTGGAGTA CCTGCTGCACCTTGGTTCTCCCCAGGACAGAGTGTCTGCTCAGCAGCAGCTGTGGATGGTGGAAGACACG CTGGCAGGTCTGGGTGGCCCCCAGAAACCGCCCCCACACACTGAGCCTGACTCCCCATCTCCCGTGCTCC AGGGCGAGGAGTCCTCAGAGAGGGAGAGCCTGCCAGAGTCCTTGGAACTGAGCTCCCCTAGGTCCCCCGA GACTGACTGGGGGCGGCCTCCTGGAGGCGACAAAGACCTCGCCAGCCCTCACTTAGGTCTTGGGTCTCCG AGGGTCTCCCGGGCTTCCAGCCCTGAGGGTCGCCACCTCCCTTCCCCACAGCTAGGAACCAAGGCCCCGG TGGCCCGGCCCCGGATGAGTGCCCAGGAGCAGCTGGAGCGGATGCGCAGAAACCAGGAATGTGGACGGCC CTTCCCTCGCCCGACCTCCCCCCGGCTTCTCACCCTGGGAAGGACACTGTCCCCAGCCAGACGCCAGCCT GACGTGGAGCAAAGGCCTGTCGTAGGACACTCGGGAGCCCAGAAATGGCTCAGAAGCTCTGGGTCCTGGA GTAGTCCAAGGAACACCACCCCTTACTTGCCGACTTCCGAAGGTCACCGGGAGCGGGTTCTCAGCCTCTC CCAAGCCCTGGCTACTGAGGCGTCGCAGTGGCACAGAATGATGACAGGTGGAAATTTGGACTCCCAGGGA GACCCTCTTCCCGGTGTGCCGCTGCCTCCTTCGGACCCCACGCGCCAGGAGACCCCTCCCCCCAGATCTC CCCCGGTGGCTAATTCGGGTTCCACGGGGTTCTCTCGCCGAGGGAGTGGGCGTGGAGGAGGTCCCACCCC CTGGGGGCCCGCGTGGGATGCCGGGATCGCCCCTCCGGTCCTGCCACAAGACGAGGGGGCATGGCCTCTG CGAGTCACTCTGCTACAATCCAGCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203633 protein sequence
Red=Cloning site Green=Tags(s) MEGSRPRSSLSLASSASTISSLSSLSPKKPTRAVNKIHAFGKRGNALRRDPNLPVHIRGWLHKQDSSGLR LWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYNIRPDGPGAPRGRRFTFTAEHPGMRTYVLAADTLED LRGWLRALGRASRAEGDDYGQPRSPARPQPGEGPGGPGGPPEVSRGEEGRISESPEVTRLSRGRGRPRLL TPSPTTDLHSGLQMRRARSPDLFTPLSRPPSPLSLPRPRSAPARRPPAPSGDTAPPARPHTPLSRIDVRP PLDWGPQRQTLSRPPTPRRGPPSEAGGGKPPRSPQHWSQEPRTQAHSGSPTYLQLPPRPPGTRASMVLLP GPPLESTFHQSLETDTLLTKLCGQDRLLRRLQEEIDQKQEEKEQLEAALELTRQQLGQATREAGAPGRAW GRQRLLQDRLVSVRATLCHLTQERERVWDTYSGLEQELGTLRETLEYLLHLGSPQDRVSAQQQLWMVEDT LAGLGGPQKPPPHTEPDSPSPVLQGEESSERESLPESLELSSPRSPETDWGRPPGGDKDLASPHLGLGSP RVSRASSPEGRHLPSPQLGTKAPVARPRMSAQEQLERMRRNQECGRPFPRPTSPRLLTLGRTLSPARRQP DVEQRPVVGHSGAQKWLRSSGSWSSPRNTTPYLPTSEGHRERVLSLSQALATEASQWHRMMTGGNLDSQG DPLPGVPLPPSDPTRQETPPPRSPPVANSGSTGFSRRGSGRGGGPTPWGPAWDAGIAPPVLPQDEGAWPL RVTLLQSSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020904 |
| ORF Size | 2337 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020904.3 |
| RefSeq Size | 3104 bp |
| RefSeq ORF | 2340 bp |
| Locus ID | 57664 |
| UniProt ID | Q9H4M7 |
| MW | 85.4 kDa |
| Gene Summary | This gene encodes a pleckstrin homology (PH) domain-containing protein. The PH domain is found near the N-terminus and contains a putative phosphatidylinositol 3, 4, 5-triphosphate-binding motif (PPBM). Elevated expression of this gene has been observed in some melanomas. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203633L3 | Lenti ORF clone of Human pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4 (PLEKHA4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203633L4 | Lenti ORF clone of Human pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4 (PLEKHA4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG203633 | PLEKHA4 (tGFP-tagged) - Human pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4 (PLEKHA4), transcript variant 1 |
CNY 4,370.00 |
|
| SC122898 | PLEKHA4 (untagged)-Human pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4 (PLEKHA4), transcript variant 1 |
CNY 7,220.00 |
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