KCNK13 (NM_022054) Human Tagged ORF Clone

CAT#: RC203627

KCNK13 (Myc-DDK-tagged)-Human potassium channel, subfamily K, member 13 (KCNK13)

ORF Plasmid: DDK tGFP



  "NM_022054" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-KCNK13 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms K2p13.1; THIK-1; THIK1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203627 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGCCGGGGTTTCAGCTGGGGCCCGGGCCACCTGAACGAGGACAACGCGCGCTTTCTGCTGCTGG
CCGCGCTCATCGTGCTCTACCTGCTGGGCGGCGCCGCCGTCTTCTCCGCGCTGGAGCTGGCGCACGAGCG
CCAGGCCAAGCAGCGCTGGGAGGAGCGCCTGGCCAACTTCAGCCGCGGCCACAACCTGAGCCGCGACGAG
CTGCGCGGCTTCCTCCGCCACTACGAGGAGGCCACTCGGGCCGGCATCCGCGTGGACAACGTCCGCCCGC
GCTGGGACTTCACCGGCGCCTTCTACTTCGTGGGCACCGTCGTTTCCACCATAGGGTTTGGGATGACAAC
TCCGGCGACAGTAGGAGGAAAAATCTTTCTGATCTTTTACGGCCTTGTTGGGTGTTCCAGCACCATCTTG
TTCTTCAACCTCTTCCTGGAGCGCCTGATCACCATCATCGCCTACATCATGAAGTCGTGCCACCAGCGGC
AGCTCCGGAGACGAGGGGCCCTGCCCCAGGAGAGCCTGAAGGATGCGGGGCAGTGTGAGGTGGACAGCCT
GGCCGGCTGGAAGCCCTCCGTGTACTACGTCATGCTGATCCTATGCACAGCCTCCATCCTCATCTCTTGC
TGCGCCTCAGCCATGTACACCCCCATTGAAGGCTGGAGCTACTTTGACTCACTCTACTTCTGTTTTGTGG
CTTTCAGCACCATTGGCTTTGGGGACCTGGTCAGCAGCCAGAACGCCCACTATGAGAGCCAAGGCCTCTA
TCGCTTTGCCAACTTCGTCTTCATCCTCATGGGTGTCTGCTGCATCTACTCCTTGTTCAATGTCATCTCT
ATCCTCATCAAACAGTCCTTGAACTGGATCCTGAGGAAAATGGACAGCGGGTGCTGCCCGCAATGCCAGA
GAGGACTCTTGCGATCACGCAGGAACGTGGTGATGCCAGGCAGCGTCCGGAACCGCTGCAACATCTCCAT
AGAGACAGACGGGGTGGCAGAGAGTGACACGGACGGGCGCCGGCTCTCAGGGGAGATGATCTCCATGAAG
GACTTGCTGGCAGCCAACAAGGCCTCGTTGGCCATCCTGCAGAAGCAACTGTCTGAGATGGCCAACGGCT
GCCCCCACCAGACCAGCACACTGGCCCGGGACAATGAATTCTCAGGGGGGGTGGGAGCCTTTGCAATCAT
GAACAACAGGTTGGCAGAGACCAGTGGGGACAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203627 protein sequence
Red=Cloning site Green=Tags(s)

MAGRGFSWGPGHLNEDNARFLLLAALIVLYLLGGAAVFSALELAHERQAKQRWEERLANFSRGHNLSRDE
LRGFLRHYEEATRAGIRVDNVRPRWDFTGAFYFVGTVVSTIGFGMTTPATVGGKIFLIFYGLVGCSSTIL
FFNLFLERLITIIAYIMKSCHQRQLRRRGALPQESLKDAGQCEVDSLAGWKPSVYYVMLILCTASILISC
CASAMYTPIEGWSYFDSLYFCFVAFSTIGFGDLVSSQNAHYESQGLYRFANFVFILMGVCCIYSLFNVIS
ILIKQSLNWILRKMDSGCCPQCQRGLLRSRRNVVMPGSVRNRCNISIETDGVAESDTDGRRLSGEMISMK
DLLAANKASLAILQKQLSEMANGCPHQTSTLARDNEFSGGVGAFAIMNNRLAETSGDR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022054
ORF Size 1224 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_022054.4
RefSeq Size 2587 bp
RefSeq ORF 1227 bp
Locus ID 56659
UniProt ID Q9HB14
Domains ion_trans
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
MW 45.4 kDa
Gene Summary Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a potassium channel containing two pore-forming domains. This protein is an open channel that can be stimulated by arachidonic acid and inhibited by the anesthetic halothane. [provided by RefSeq, Jul 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...