VILIP3 (HPCAL1) (NM_002149) Human Tagged ORF Clone
CAT#: RC203570
HPCAL1 (Myc-DDK-tagged)-Human hippocalcin-like 1 (HPCAL1), transcript variant 1
ORF Plasmid: tGFP
"NM_002149" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BDR1; HLP2; VILIP-3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203570 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAAACAGAACAGCAAGCTGCGGCCCGAGGTGCTGCAGGACCTGCGGGAGAACACGGAGTTCACCG ACCACGAGCTGCAGGAGTGGTACAAGGGCTTCCTCAAGGACTGCCCCACCGGCCACCTGACCGTGGACGA GTTCAAGAAGATCTACGCCAACTTCTTCCCCTACGGCGACGCTTCCAAGTTCGCCGAGCACGTCTTCCGC ACCTTCGACACCAACGGCGACGGCACCATCGACTTCCGGGAGTTCATCATTGCGCTGAGCGTGACCTCGC GGGGCAAGCTGGAGCAGAAGCTCAAGTGGGCCTTCAGCATGTACGACCTGGACGGCAACGGCTACATCAG CCGCAGCGAGATGCTGGAGATCGTGCAGGCCATCTACAAGATGGTGTCGTCTGTGATGAAGATGCCGGAG GATGAGTCCACCCCGGAGAAGCGCACAGACAAGATCTTCAGGCAGATGGACACCAACAATGACGGCAAAC TGTCCTTGGAAGAATTCATCAGAGGTGCCAAGAGCGACCCCTCCATCGTCCGCCTGCTGCAGTGCGACCC CAGCAGTGCCAGTCAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203570 protein sequence
Red=Cloning site Green=Tags(s) MGKQNSKLRPEVLQDLRENTEFTDHELQEWYKGFLKDCPTGHLTVDEFKKIYANFFPYGDASKFAEHVFR TFDTNGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISRSEMLEIVQAIYKMVSSVMKMPE DESTPEKRTDKIFRQMDTNNDGKLSLEEFIRGAKSDPSIVRLLQCDPSSASQF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002149 |
ORF Size | 579 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002149.4 |
RefSeq Size | 1779 bp |
RefSeq ORF | 582 bp |
Locus ID | 3241 |
UniProt ID | P37235 |
Domains | EFh |
MW | 22.3 kDa |
Gene Summary | The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. Several alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Apr 2012] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis
,null,
Autophagy
,PubMed ID 35403545
[VILIP3]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203570L3 | Lenti ORF clone of Human hippocalcin-like 1 (HPCAL1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203570L4 | Lenti ORF clone of Human hippocalcin-like 1 (HPCAL1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG203570 | HPCAL1 (tGFP-tagged) - Human hippocalcin-like 1 (HPCAL1), transcript variant 1 |
CNY 4,000.00 |
|
SC118780 | HPCAL1 (untagged)-Human hippocalcin-like 1 (HPCAL1), transcript variant 1 |
CNY 2,400.00 |