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Tuftelin 1 (TUFT1) (NM_020127) Human Tagged ORF Clone
CAT＃: RC203420
TUFT1 (Myc-DDK-tagged)-Human tuftelin 1 (TUFT1), transcript variant 1
ORF Plasmid: tGFP
"NM_020127" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203420 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGGGACGCGGAACTGGTGTACCCTGGTGGACGTGCACCCAGAGGACCAGGCGGCGGGCAGCGTGG ACATTCTCAGGCTGACTCTCCAGGGTGAACTGACAGGAGATGAACTTGAACACATAGCCCAGAAGGCGGG CAGGAAGACCTATGCCATGGTGTCCAGCCACTCAGCTGGTCATTCTCTGGCTTCAGAACTGGTGGAGTCC CATGATGGACATGAGGAGATCATTAAGGTGTACTTGAAGGGGAGGTCTGGAGACAAGATGATTCACGAGA AGAATATTAACCAGCTGAAGAGTGAGGTCCAGTACATCCAGGAGGCCAGGAACTGCCTACAGAAGCTCCG GGAGGATATAAGTAGCAAGCTTGACAGGAACCTAGGAGATTCTCTCCATCGACAGGAGATACAGGTGGTG CTAGAAAAGCCAAATGGCTTTAGTCAGAGTCCCACAGCCCTGTACAGCAGCCCACCTGAGGTGGACACCT GTATAAATGAGGATGTTGAGAGCTTGAGGAAGACGGTGCAGGACTTGCTGGCCAAGCTTCAGGAGGCCAA GCGGCAACACCAGTCAGACTGTGTGGCTTTTGAGGTCACACTCAGCCGGTACCAGAGGGAAGCAGAACAA AGTAATGTGGCCCTTCAGAGAGAGGAGGACAGAGTGGAGCAGAAAGAGGCAGAAGTCGGAGAGCTGCAGA GGCGCTTGCTAGGGATGGAGACGGAGCATCAGGCCTTACTGGCGAAAGTGAGGGAAGGGGAGGTGGCCCT AGAGGAACTTCGGAGCAACAATGCTGACTGCCAAGCAGAACGAGAAAAGGCTGCTACCCTGGAAAAGGAA GTGGCCGGGTTGCGGGAGAAGATCCACCACTTGGATGACATGCTCAAGAGCCAGCAGCGGAAAGTCCGGC AAATGATAGAGCAGCTCCAGAATTCAAAAGCTGTGATCCAGTCAAAGGACGCCACCATCCAGGAGCTCAA GGAGAAAATCGCCTATCTGGAGGCAGAGAATTTAGAGATGCATGACCGGATGGAACACCTGATAGAAAAA CAAATCAGTCATGGCAACTTCAGCACCCAGGCCCGGGCCAAGACAGAGAACCCGGGCAGTATTAGGATAT CCAAGCCGCCTAGCCCGAAGCCCATGCCTGTCATCCGAGTGGTGGAAACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203420 protein sequence
Red=Cloning site Green=Tags(s) MNGTRNWCTLVDVHPEDQAAGSVDILRLTLQGELTGDELEHIAQKAGRKTYAMVSSHSAGHSLASELVES HDGHEEIIKVYLKGRSGDKMIHEKNINQLKSEVQYIQEARNCLQKLREDISSKLDRNLGDSLHRQEIQVV LEKPNGFSQSPTALYSSPPEVDTCINEDVESLRKTVQDLLAKLQEAKRQHQSDCVAFEVTLSRYQREAEQ SNVALQREEDRVEQKEAEVGELQRRLLGMETEHQALLAKVREGEVALEELRSNNADCQAEREKAATLEKE VAGLREKIHHLDDMLKSQQRKVRQMIEQLQNSKAVIQSKDATIQELKEKIAYLEAENLEMHDRMEHLIEK QISHGNFSTQARAKTENPGSIRISKPPSPKPMPVIRVVET TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020127 |
| ORF Size | 1170 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020127.3 |
| RefSeq Size | 3127 bp |
| RefSeq ORF | 1173 bp |
| Locus ID | 7286 |
| UniProt ID | Q9NNX1 |
| Protein Families | Secreted Protein |
| MW | 44.3 kDa |
| Gene Summary | Tuftelin is an acidic protein that is thought to play a role in dental enamel mineralization and is implicated in caries susceptibility. It is also thought to be involved with adaptation to hypoxia, mesenchymal stem cell function, and neurotrophin nerve growth factor mediated neuronal differentiation. [provided by RefSeq, Aug 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203420L3 | Lenti ORF clone of Human tuftelin 1 (TUFT1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203420L4 | Lenti ORF clone of Human tuftelin 1 (TUFT1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG203420 | TUFT1 (tGFP-tagged) - Human tuftelin 1 (TUFT1), transcript variant 1 |
CNY 4,370.00 |
|
| SC113182 | TUFT1 (untagged)-Human tuftelin 1 (TUFT1), transcript variant 1 |
CNY 3,656.00 |
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