DPP3 (NM_130443) Human Tagged ORF Clone

CAT#: RC203406

DPP3 (Myc-DDK-tagged)-Human dipeptidyl-peptidase 3 (DPP3), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_130443" in other vectors (5)

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CNY 5,400.00

CNY 5,800.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


DPP3 mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms DPPIII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203406 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACACCCAGTACATCCTGCCCAATGACATCGGCGTGTCTAGCCTGGACTGCCGTGAGGCCTTCC
GCCTGCTGTCACCCACAGAGCGCCTCTATGCCTACCACCTGTCCCGTGCCGCCTGGTACGGAGGCCTGGC
TGTGCTGCTTCAGACCTCCCCTGAGGCCCCCTACATCTATGCTCTGCTCAGCCGCCTCTTCCGCGCCCAG
GACCCCGACCAGCTGCGCCAACATGCCCTGGCTGAAGGCCTTACCGAGGAGGAGTATCAGGCGTTCCTGG
TCTATGCCGCGGGTGTTTACTCCAACATGGGCAACTACAAGTCCTTTGGTGACACCAAGTTTGTTCCCAA
CTTGCCCAAGGAAAAGCTGGAACGGGTGATCCTAGGGAGTGAGGCTGCTCAGCAGCACCCAGAAGAAGTC
AGGGGCCTCTGGCAGACCTGCGGGGAGCTTATGTTCTCTCTGGAGCCAAGGCTTCGACACCTCGGACTGG
GGAAGGAGGGAATCACCACCTATTTCTCTGGGAATTGTACCATGGAAGATGCCAAATTGGCCCAGGACTT
TCTGGACTCACAGAACCTCAGTGCCTACAACACCCGGCTCTTCAAAGAGGTCGATGGAGAAGGGAAGCCC
TACTACGAGGTGCGGCTGGCTTCTGTGCTTGGCTCAGAGCCTTCCCTGGACTCTGAGGTGACTTCCAAGC
TGAAGAGCTATGAATTCCGGGGAAGCCCTTTCCAGGTGACCCGGGGGGACTACGCGCCCATCCTCCAGAA
GGTGGTGGAGCAGCTGGAGAAAGCCAAGGCCTATGCAGCCAACAGCCACCAGGGGCAGATGCTGGCCCAG
TATATAGAGAGCTTCACCCAGGGCTCCATCGAGGCCCACAAGAGGGGCTCCCGCTTCTGGATCCAGGACA
AAGGCCCCATCGTGGAGAGTTACATCGGGTTCATCGAGAGCTACCGCGACCCCTTTGGTTCCCGAGGAGA
ATTTGAAGGTTTCGTAGCTGTGGTGAACAAGGCCATGAGTGCCAAGTTTGAGCGGCTGGTGGCGAGCGCA
GAGCAGCTGCTGAAGGAGCTGCCCTGGCCCCCAACCTTTGAGAAGGACAAGTTCCTCACCCCTGACTTCA
CCTCCCTGGATGTTCTCACCTTCGCTGGCTCCGGCATCCCTGCCGGCATCAACATCCCCAACTACGATGA
TCTGAGGCAGACGGAAGGCTTTAAGAACGTGTCGCTGGGGAATGTGCTGGCTGTGGCCTACGCCACGCAG
CGGGAGAAGCTTACCTTTCTGGAGGAGGATGACAAGGACCTGTACATCCTCTGGAAGGGGCCCTCCTTCG
ATGTGCAGGTGGGCCTGCACGAGCTGCTGGGCCATGGCAGTGGCAAGCTCTTCGTACAGGACGAAAAAGG
AGCATTCAACTTTGACCAGGAAACAGTGATCAACCCAGAGACGGGCGAGCAGATTCAGAGCTGGTATCGG
AGCGGGGAGACCTGGGATAGCAAGTTCAGCACCATCGCCTCCAGCTACGAAGAGTGCCGGGCTGAGAGCG
TGGGTCTCTACCTCTGTCTCCACCCGCAAGTGCTGGAGATCTTTGGCTTTGAGGGGGCTGATGCGGAGGA
CGTGATCTACGTGAACTGGCTCAACATGGTTCGGGCCGGGCTGCTCGCTCTGGAGTTCTACACACCTGAG
GCCTTCAACTGGCGACAGGCCCATATGCAGGCCCGGTTTGTGATCCTGAGAGTCTTGCTGGAGGCTGGCG
AGGGACTCGTTACCATCACTCCCACCACAGGCTCCGATGGGCGCCCAGATGCCCGGGTCCGCCTCGACCG
CAGCAAGATCCGGTCTGTGGGCAAGCCTGCTCTAGAGCGCTTCCTGCGGAGACTTCAGGTGCTGAAGTCC
ACAGGGGATGTGGCCGGAGGGCGGGCCCTGTACGAGGGGTATGCAACGGTCACTGATGCGCCCCCCGAGT
GCTTCCTCACCCTCAGGGACACGGTGCTGCTGCGTAAGGAATCTCGGAAGCTCATTGTTCAGCCCAACAC
TCGCCTTGAAGGCTCAGACGTGCAGCTTCTGGAATACGAGGCGTCAGCTGCTGGCCTCATCCGATCCTTC
TCTGAGCGTTTCCCAGAGGATGGACCCGAGTTGGAGGAGATCCTCACACAGCTGGCCACAGCCGATGCCC
GATTCTGGAAGGGCCCCAGTGAGGCCCCATCTGGCCAAGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203406 protein sequence
Red=Cloning site Green=Tags(s)

MADTQYILPNDIGVSSLDCREAFRLLSPTERLYAYHLSRAAWYGGLAVLLQTSPEAPYIYALLSRLFRAQ
DPDQLRQHALAEGLTEEEYQAFLVYAAGVYSNMGNYKSFGDTKFVPNLPKEKLERVILGSEAAQQHPEEV
RGLWQTCGELMFSLEPRLRHLGLGKEGITTYFSGNCTMEDAKLAQDFLDSQNLSAYNTRLFKEVDGEGKP
YYEVRLASVLGSEPSLDSEVTSKLKSYEFRGSPFQVTRGDYAPILQKVVEQLEKAKAYAANSHQGQMLAQ
YIESFTQGSIEAHKRGSRFWIQDKGPIVESYIGFIESYRDPFGSRGEFEGFVAVVNKAMSAKFERLVASA
EQLLKELPWPPTFEKDKFLTPDFTSLDVLTFAGSGIPAGINIPNYDDLRQTEGFKNVSLGNVLAVAYATQ
REKLTFLEEDDKDLYILWKGPSFDVQVGLHELLGHGSGKLFVQDEKGAFNFDQETVINPETGEQIQSWYR
SGETWDSKFSTIASSYEECRAESVGLYLCLHPQVLEIFGFEGADAEDVIYVNWLNMVRAGLLALEFYTPE
AFNWRQAHMQARFVILRVLLEAGEGLVTITPTTGSDGRPDARVRLDRSKIRSVGKPALERFLRRLQVLKS
TGDVAGGRALYEGYATVTDAPPECFLTLRDTVLLRKESRKLIVQPNTRLEGSDVQLLEYEASAAGLIRSF
SERFPEDGPELEEILTQLATADARFWKGPSEAPSGQA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_130443
ORF Size 2211 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_130443.4
RefSeq Size 3101 bp
RefSeq ORF 2214 bp
Locus ID 10072
UniProt ID Q9NY33
Domains Peptidase_M49
Protein Families Druggable Genome, Protease
MW 82.6 kDa
Gene Summary This gene encodes a protein that is a member of the M49 family of metallopeptidases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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