BIN3 (NM_018688) Human Tagged ORF Clone

CAT#: RC203378

BIN3 (Myc-DDK-tagged)-Human bridging integrator 3 (BIN3)

ORF Plasmid: DDK tGFP



  "NM_018688" in other vectors (6)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


BIN3 mouse monoclonal antibody, clone OTI8E9 (formerly 8E9)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203378 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTGGATTCCTTTTAAGATTGGGCAGCCCAAGAAACAGATTGTGCCCAAAACAGTGGAGAGAGACT
TTGAAAGGGAGTATGGAAAACTTCAGCAGCTGGAAGAGCAGACCCGGAGGCTGCAGAAAGACATGAAGAA
GAGCACCGACGCAGACCTGGCCATGTCAAAATCTGCCGTGAAGATATCCTTGGACTTACTCTCCAATCCC
CTCTGTGAGCAAGACCAGGACCTTCTGAACATGGTGACGGCCCTGGACACGGCCATGAAGCGGATGGATG
CCTTCAATCAGGAAAAGGTGAACCAGATCCAGAAGACTGTGATCGAGCCCTTAAAAAAGTTCGGCAGTGT
CTTCCCGAGCCTCAACATGGCTGTGAAGAGGCGGGAACAGGCCTTGCAGGACTACAGGAGGCTGCAGGCC
AAGGTGGAGAAGTATGAGGAAAAGGAGAAGACGGGGCCAGTGCTGGCCAAGCTCCACCAGGCACGAGAGG
AGCTGCGGCCTGTGCGGGAGGACTTTGAAGCCAAGAACAGGCAGCTGCTGGAGGAGATGCCGCGCTTCTA
CGGCAGCCGCCTCGACTACTTCCAGCCCAGCTTTGAGTCCCTCATCCGAGCTCAGGTTGTGTACTACTCG
GAAATGCACAAGATCTTTGGAGACCTGTCCCATCAGCTTGACCAGCCAGGCCACTCCGATGAGCAGCGGG
AGCGGGAGAACGAGGCCAAACTCAGTGAGCTCCGGGCCCTCTCCATTGTGGCCGATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203378 protein sequence
Red=Cloning site Green=Tags(s)

MSWIPFKIGQPKKQIVPKTVERDFEREYGKLQQLEEQTRRLQKDMKKSTDADLAMSKSAVKISLDLLSNP
LCEQDQDLLNMVTALDTAMKRMDAFNQEKVNQIQKTVIEPLKKFGSVFPSLNMAVKRREQALQDYRRLQA
KVEKYEEKEKTGPVLAKLHQAREELRPVREDFEAKNRQLLEEMPRFYGSRLDYFQPSFESLIRAQVVYYS
EMHKIFGDLSHQLDQPGHSDEQRERENEAKLSELRALSIVADD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018688
ORF Size 759 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018688.3, NP_061158.1
RefSeq Size 1863 bp
RefSeq ORF 762 bp
Locus ID 55909
UniProt ID Q9NQY0
Domains BAR, BAR
MW 29.7 kDa
Gene Summary The product of this gene is a member of the BAR domain protein family. The encoded protein is comprised solely of a BAR domain which is predicted to form coiled-coil structures and proposed to mediate dimerization, sense and induce membrane curvature, and bind small GTPases. BAR domain proteins have been implicated in endocytosis, intracellular transport, and a diverse set of other processes. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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