United States
Germany
Japan
United Kingdom
China
SIAH Interacting Protein (CACYBP) (NM_014412) Human Tagged ORF Clone
CAT＃: RC203302
CACYBP (Myc-DDK-tagged)-Human calcyclin binding protein (CACYBP), transcript variant 1
ORF Plasmid: tGFP
"NM_014412" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GIG5; PNAS-107; S100A6BP; SIP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203302 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCAGAAGAGCTACAGAAAGATCTAGAAGAGGTAAAGGTGTTGCTGGAAAAGGCTACTAGGAAAA GAGTACGTGATGCCCTTACAGCTGAAAAATCCAAGATTGAGACAGAAATCAAGAACAAGATGCAACAGAA ATCACAGAAGAAAGCAGAACTTCTTGATAATGAAAAACCAGCTGCTGTGGTTGCTCCCATTACAACGGGC TATACGGTGAAAATCAGTAATTATGGATGGGATCAGTCAGATAAGTTTGTGAAAATCTACATTACCTTAA CTGGAGTTCATCAAGTTCCCACTGAGAATGTGCAGGTGCATTTCACAGAGAGGTCATTTGATCTTTTGGT AAAGAATCTAAATGGGAAGAGTTACTCCATGATTGTGAACAATCTCTTGAAACCCATCTCTGTGGAAGGC AGTTCAAAAAAAGTCAAGACTGATACAGTTCTTATATTGTGTAGAAAGAAAGTGGAAAACACAAGGTGGG ATTACCTGACCCAGGTTGAAAAGGAGTGCAAAGAAAAAGAGAAGCCCTCCTATGACACTGAAACAGATCC TAGTGAGGGATTGATGAATGTTCTAAAGAAAATTTATGAAGATGGAGACGATGATATGAAGCGAACCATT AATAAAGCCTGGGTGGAATCAAGAGAGAAGCAAGCCAAAGGAGACACGGAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203302 protein sequence
Red=Cloning site Green=Tags(s) MASEELQKDLEEVKVLLEKATRKRVRDALTAEKSKIETEIKNKMQQKSQKKAELLDNEKPAAVVAPITTG YTVKISNYGWDQSDKFVKIYITLTGVHQVPTENVQVHFTERSFDLLVKNLNGKSYSMIVNNLLKPISVEG SSKKVKTDTVLILCRKKVENTRWDYLTQVEKECKEKEKPSYDTETDPSEGLMNVLKKIYEDGDDDMKRTI NKAWVESREKQAKGDTEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014412 |
| ORF Size | 684 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014412.3 |
| RefSeq Size | 3060 bp |
| RefSeq ORF | 687 bp |
| Locus ID | 27101 |
| UniProt ID | Q9HB71 |
| Domains | SGS |
| Protein Pathways | Wnt signaling pathway |
| MW | 26.2 kDa |
| Gene Summary | The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203302L3 | Lenti ORF clone of Human calcyclin binding protein (CACYBP), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203302L4 | Lenti ORF clone of Human calcyclin binding protein (CACYBP), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG203302 | CACYBP (tGFP-tagged) - Human calcyclin binding protein (CACYBP), transcript variant 1 |
CNY 4,000.00 |
|
| SC115012 | CACYBP (untagged)-Human calcyclin binding protein (CACYBP), transcript variant 1 |
CNY 2,400.00 |
其它SIAH Interacting Protein产品
