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MAGP2 (MFAP5) (NM_003480) Human Tagged ORF Clone
CAT＃: RC203242
MFAP5 (Myc-DDK-tagged)-Human microfibrillar associated protein 5 (MFAP5)
ORF Plasmid: tGFP
"NM_003480" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AAT9; MAGP-2; MAGP2; MFAP-5; MP25 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203242 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTCTTGGGACCCAAGGTGCTGCTGTTTCTTGCTGCATTCATCATCACCTCTGACTGGATACCCC TGGGGGTCAATAGTCAACGAGGAGACGATGTGACTCAAGCGACTCCAGAAACATTCACAGAAGATCCTAA TCTGGTGAATGATCCCGCTACAGATGAAACAGTTTTGGCTGTTTTGGCTGATATTGCACCTTCCACAGAT GACTTGGCCTCCCTCAGTGAAAAAAATACCACTGCAGAGTGCTGGGATGAGAAATTTACCTGCACAAGGC TCTACTCTGTGCATCGGCCGGTTAAACAATGCATTCATCAGTTATGCTTCACCAGTTTACGACGTATGTA CATCGTCAACAAGGAGATCTGCTCTCGTCTTGTCTGTAAGGAACACGAAGCTATGAAAGATGAGCTTTGC CGTCAGATGGCTGGTCTGCCCCCTAGGAGACTCCGTCGCTCCAATTACTTCCGACTTCCTCCCTGTGAAA ATGTGGATTTGCAGAGACCCAATGGTCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203242 protein sequence
Red=Cloning site Green=Tags(s) MSLLGPKVLLFLAAFIITSDWIPLGVNSQRGDDVTQATPETFTEDPNLVNDPATDETVLAVLADIAPSTD DLASLSEKNTTAECWDEKFTCTRLYSVHRPVKQCIHQLCFTSLRRMYIVNKEICSRLVCKEHEAMKDELC RQMAGLPPRRLRRSNYFRLPPCENVDLQRPNGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003480 |
| ORF Size | 519 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003480.4 |
| RefSeq Size | 2949 bp |
| RefSeq ORF | 522 bp |
| Locus ID | 8076 |
| UniProt ID | Q13361 |
| Protein Families | Secreted Protein |
| MW | 19.6 kDa |
| Gene Summary | This gene encodes a 25-kD microfibril-associated glycoprotein which is a component of microfibrils of the extracellular matrix. The encoded protein promotes attachment of cells to microfibrils via alpha-V-beta-3 integrin. Deficiency of this gene in mice results in neutropenia. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203242L1 | Lenti ORF clone of Human microfibrillar associated protein 5 (MFAP5), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203242L2 | Lenti ORF clone of Human microfibrillar associated protein 5 (MFAP5), mGFP tagged |
CNY 5,890.00 |
|
| RC203242L3 | Lenti ORF clone of Human microfibrillar associated protein 5 (MFAP5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203242L4 | Lenti ORF clone of Human microfibrillar associated protein 5 (MFAP5), mGFP tagged |
CNY 5,890.00 |
|
| RG203242 | MFAP5 (tGFP-tagged) - Human microfibrillar associated protein 5 (MFAP5) |
CNY 4,000.00 |
|
| SC127462 | MFAP5 (untagged)-Human microfibrillar associated protein 5 (MFAP5) |
CNY 2,400.00 |
