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RNF166 (NM_178841) Human Tagged ORF Clone
CAT＃: RC203224
RNF166 (Myc-DDK-tagged)-Human ring finger protein 166 (RNF166), transcript variant 1
ORF Plasmid: tGFP
"NM_178841" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203224 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTATGTTCCGCAGCCTGGTGGCCTCGGCTCAGCAGCGGCAGCCGCCGGCCGGGCCGGCGGGCGGCG ACAGCGGCCTGGAGGCGCAGTACACCTGCCCCATCTGCCTGGAGGTCTATCACCGGCCCGTGGCCATCGG CAGCTGCGGCCACACGTTCTGCGGGGAGTGTCTCCAGCCCTGCCTGCAGGTGCCATCCCCGCTGTGCCCA CTGTGCCGCCTGCCCTTCGACCCCAAGAAGGTGGACAAGGCCACCCACGTGGAGAAGCAGCTCTCATCCT ACAAAGCGCCCTGTCGAGGCTGCAACAAAAAGGTGACCCTGGCAAAGATGAGAGTGCACATTTCGTCCTG CCTGAAGGTCCAGGAGCAGATGGCCAACTGCCCCAAGTTCGTCCCCGTGGTGCCCACATCACAGCCTATC CCCAGCAACATCCCCAACAGGTCCACCTTCGCCTGCCCGTACTGTGGTGCCCGCAACCTGGACCAGCAGG AGCTGGTGAAGCACTGTGTGGAAAGCCACCGCAGCGACCCCAACCGCGTGGTGTGCCCCATCTGCTCGGC AATGCCCTGGGGGGACCCCAGCTACAAGAGCGCCAACTTCCTGCAGCACCTGCTTCACCGACACAAGTTC TCCTACGACACCTTTGTGGACTACAGTATTGACGAGGAGGCCGCCTTCCAGGCTGCTCTGGCCCTGTCTC TCTCTGAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203224 protein sequence
Red=Cloning site Green=Tags(s) MAMFRSLVASAQQRQPPAGPAGGDSGLEAQYTCPICLEVYHRPVAIGSCGHTFCGECLQPCLQVPSPLCP LCRLPFDPKKVDKATHVEKQLSSYKAPCRGCNKKVTLAKMRVHISSCLKVQEQMANCPKFVPVVPTSQPI PSNIPNRSTFACPYCGARNLDQQELVKHCVESHRSDPNRVVCPICSAMPWGDPSYKSANFLQHLLHRHKF SYDTFVDYSIDEEAAFQAALALSLSEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178841 |
| ORF Size | 711 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178841.2, NP_849163.1 |
| RefSeq Size | 1888 bp |
| RefSeq ORF | 714 bp |
| Locus ID | 115992 |
| UniProt ID | Q96A37 |
| Protein Families | Druggable Genome |
| MW | 26.1 kDa |
| Gene Summary | E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates (PubMed:27880896). In turn, participates in different biological processes including interferon production or autophagy (PubMed:26456228, PubMed:27880896). Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6 (PubMed:26456228). Plays also a role in the early recruitment of autophagy adapters to bacteria (PubMed:27880896). Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication (PubMed:27880896).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203224L3 | Lenti ORF clone of Human ring finger protein 166 (RNF166), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203224L4 | Lenti ORF clone of Human ring finger protein 166 (RNF166), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG203224 | RNF166 (tGFP-tagged) - Human ring finger protein 166 (RNF166), transcript variant 1 |
CNY 4,000.00 |
|
| SC319295 | RNF166 (untagged)-Human ring finger protein 166 (RNF166), transcript variant 1 |
CNY 2,400.00 |
