PPAPDC3 (PLPP7) (NM_032728) Human Tagged ORF Clone

CAT#: RC202953

PPAPDC3 (Myc-DDK-tagged)-Human phosphatidic acid phosphatase type 2 domain containing 3 (PPAPDC3)

ORF Plasmid: DDK tGFP



  "NM_032728" in other vectors (4)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal PPAPDC3 Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms C9orf67; NET39; PPAPDC3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202953 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGCTTCCCAGAGCCGGGCCCGTGCCCGGGACCGCAACAACGTCCTCAACCGGGCTGAGTTCCTGT
CCCTGAACCAGCCCCCCAAGGGGGGCCCGGAGCCCCGCAGCTCGGGCAGAAAGGCCTCGGGCCCATCAGC
ACAGCCCCCACCTGCTGGTGACGGGGCCAGAGAGCGACGCCAGTCACAGCAGCTGCCAGAGGAGGACTGC
ATGCAGCTGAACCCCTCCTTCAAGGGCATCGCCTTCAACTCCCTGCTGGCCATCGATATCTGTATGTCCA
AGCGGCTGGGGGTGTGCGCTGGCCGGGCGGCGTCCTGGGCCAGTGCCCGCTCCATGGTCAAGCTCATCGG
CATCACGGGCCACGGCATCCCCTGGATCGGAGGCACCATCCTCTGCCTGGTGAAGAGCAGCACACTGGCC
GGCCAGGAGGTGCTCATGAATCTGCTCCTGGCCCTGCTCCTGGACATCATGACGGTGGCCGGCGTGCAGA
AGCTCATCAAGCGGCGCGGCCCGTACGAGATGAGCCCCAGCCTCCTGGACTACCTCACCATGGACATCTA
CGCCTTCCCGGCCGGGCACGCCAGCCGCGCCGCCATGGTGTCCAAGTTCTTCCTCAGCCACCTGGTGCTG
GCGGTGCCCCTGCGCGTGCTGCTGGTGCTCTGGGCCCTCTGCGTGGGCCTGTCCCGCGTGATGATCGGCC
GCCACCACGTCACGGACGTCCTCTCCGGCTTTGTCATCGGCTACCTCCAGTTCCGTCTGGTGGAGCTGGT
CTGGATGCCCTCCAGCACCTGCCAGATGCTCATCTCTGCCTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202953 protein sequence
Red=Cloning site Green=Tags(s)

MPASQSRARARDRNNVLNRAEFLSLNQPPKGGPEPRSSGRKASGPSAQPPPAGDGARERRQSQQLPEEDC
MQLNPSFKGIAFNSLLAIDICMSKRLGVCAGRAASWASARSMVKLIGITGHGIPWIGGTILCLVKSSTLA
GQEVLMNLLLALLLDIMTVAGVQKLIKRRGPYEMSPSLLDYLTMDIYAFPAGHASRAAMVSKFFLSHLVL
AVPLRVLLVLWALCVGLSRVMIGRHHVTDVLSGFVIGYLQFRLVELVWMPSSTCQMLISAW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032728
ORF Size 813 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_032728.2, NP_116117.2
RefSeq Size 2095 bp
RefSeq ORF 816 bp
Locus ID 84814
UniProt ID Q8NBV4
Domains acidPPc
Protein Families Transmembrane
MW 29.5 kDa
Gene Summary Plays a role as negative regulator of myoblast differentiation, in part through effects on MTOR signaling. Has no detectable enzymatic activity (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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