PITX3 (NM_005029) Human Tagged ORF Clone

CAT#: RC202902

PITX3 (Myc-DDK-tagged)-Human paired-like homeodomain 3 (PITX3)

ORF Plasmid: DDK tGFP



  "NM_005029" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


PITX3 rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ASGD1; ASMD; ASOD; CTPP4; CTRCT11; PTX3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202902 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTTCGGCCTGCTCAGCGAGGCAGAGGCCCGGAGCCCTGCCCTGTCGCTGTCAGACGCTGGCACTC
CGCACCCCCAGCTCCCAGAGCACGGCTGCAAGGGCCAGGAGCACAGCGACTCAGAAAAGGCCTCGGCTTC
GCTGCCCGGCGGCTCCCCAGAGGACGGTTCGCTGAAAAAGAAGCAGCGGCGGCAGCGCACGCACTTCACC
AGCCAGCAGCTACAGGAGCTAGAGGCGACCTTCCAGAGGAACCGCTACCCCGACATGAGCACGCGCGAGG
AGATTGCCGTGTGGACCAACCTCACCGAGGCCCGCGTGCGGGTGTGGTTCAAGAACCGGCGCGCCAAATG
GCGGAAGCGCGAGCGCAGCCAGCAGGCCGAGCTATGCAAAGGCAGCTTCGCGGCGCCGCTCGGGGGGCTG
GTGCCGCCCTACGAGGAGGTGTACCCCGGCTACTCGTACGGCAACTGGCCGCCCAAGGCTCTTGCCCCGC
CGCTCGCCGCCAAGACCTTTCCATTCGCCTTCAACTCGGTCAACGTGGGGCCTCTGGCTTCGCAGCCCGT
CTTCTCGCCACCCAGCTCCATCGCCGCCTCCATGGTGCCCTCCGCCGCGGCTGCCCCGGGCACCGTGCCA
GGGCCTGGGGCCCTGCAGGGCCTGGGCGGGGGCCCCCCCGGGCTGGCTCCGGCCGCCGTGTCCTCCGGGG
CCGTGTCCTGCCCTTATGCCTCGGCCGCCGCCGCCGCCGCGGCTGCCGCCTCTTCCCCCTACGTCTATCG
GGACCCGTGTAACTCGAGCCTGGCCAGCCTGCGGCTCAAAGCCAAACAGCACGCCTCCTTCAGCTACCCC
GCTGTGCACGGGCCGCCCCCGGCAGCCAACCTTAGTCCGTGCCAGTACGCCGTGGAAAGGCCCGTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202902 protein sequence
Red=Cloning site Green=Tags(s)

MEFGLLSEAEARSPALSLSDAGTPHPQLPEHGCKGQEHSDSEKASASLPGGSPEDGSLKKKQRRQRTHFT
SQQLQELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWFKNRRAKWRKRERSQQAELCKGSFAAPLGGL
VPPYEEVYPGYSYGNWPPKALAPPLAAKTFPFAFNSVNVGPLASQPVFSPPSSIAASMVPSAAAAPGTVP
GPGALQGLGGGPPGLAPAAVSSGAVSCPYASAAAAAAAAASSPYVYRDPCNSSLASLRLKAKQHASFSYP
AVHGPPPAANLSPCQYAVERPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005029
ORF Size 906 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005029.2
RefSeq Size 1407 bp
RefSeq ORF 909 bp
Locus ID 5309
UniProt ID O75364
Protein Families Druggable Genome, Transcription Factors
MW 31.8 kDa
Gene Summary This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family act as transcription factors. This protein is involved in lens formation during eye development. Mutations of this gene have been associated with anterior segment mesenchymal dysgenesis and congenital cataracts. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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