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PFKL (NM_001002021) Human Tagged ORF Clone
CAT＃: RC202888
PFKL (Myc-DDK-tagged)-Human phosphofructokinase, liver (PFKL), transcript variant 1
ORF Plasmid: tGFP
"NM_001002021" in other vectors (4)
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CNY 6,056.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATP-PFK; PFK-B; PFK-L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202888 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTAACCAGGGTAGAGGTCGAGAGTCCTCTCGTGGGGGTCTCCATGTTCAAGGGAGCTGCCGAGGCT TGAGCAGGAGCCCCCAGCAGGAAACTGGCTTTGCCAAGGCCCCCGCTGGGACAGACTGTTTCTTTCACTG CAGTCCTGGGAGCCGAGGGCAAGGGGACAGGAAAGAGGAAGTGACCTCAGAGCCTGGTGGCACCAGCATC ATGTCCAGGCTGGGGGGCATGAACGCTGCTGTCCGGGCTGTGACGCGCATGGGCATTTATGTGGGTGCCA AAGTCTTCCTCATCTACGAGGGCTATGAGGGCCTCGTGGAGGGAGGTGAGAACATCAAGCAGGCCAACTG GCTGAGCGTCTCCAACATCATCCAGCTGGGCGGCACTATCATTGGCAGCGCTCGCTGCAAGGCCTTTACC ACCAGGGAGGGGCGCCGGGCAGCGGCCTACAACCTGGTCCAGCACGGCATCACCAACCTGTGCGTCATCG GCGGGGATGGCAGCCTTACAGGTGCCAACATCTTCCGCAGCGAGTGGGGCAGCCTGCTGGAGGAGCTGGT GGCGGAAGGTAAGATCTCAGAGACTACAGCCCGGACCTACTCGCACCTGAACATCGCGGGCCTAGTGGGC TCCATCGATAACGACTTCTGCGGCACCGACATGACCATCGGCACGGACTCGGCCCTCCACCGCATCATGG AGGTCATCGATGCCATCACCACCACTGCCCAGAGCCACCAGAGGACCTTCGTGCTGGAAGTGATGGGCCG GCACTGCGGGTACCTGGCGCTGGTATCTGCACTGGCCTCAGGGGCCGACTGGCTGTTCATCCCCGAGGCT CCACCCGAGGACGGCTGGGAGAACTTCATGTGTGAGAGGCTGGGTGAGACTCGGAGCCGTGGGTCCCGAC TGAACATCATCATCATCGCTGAGGGTGCCATTGACCGCAACGGGAAGCCCATCTCGTCCAGCTACGTGAA GGACCTGGTGGTTCAGAGGCTGGGCTTCGACACCCGTGTAACTGTGCTGGGCCACGTGCAGCGGGGAGGG ACGCCCTCTGCCTTCGACCGGATCCTGAGCAGCAAGATGGGCATGGAGGCGGTGATGGCGCTGCTGGAAG CCACGCCTGACACGCCGGCCTGCGTGGTCACCCTCTCGGGGAACCAGTCAGTGCGGCTGCCCCTCATGGA GTGCGTGCAGATGACCAAGGAAGTGCAGAAAGCCATGGATGACAAGAGGTTTGACGAGGCCACCCAGCTC CGTGGTGGGAGCTTCGAGAACAACTGGAACATTTACAAGCTCCTCGCCCACCAGAAGCCCCCCAAGGAGA AGTCTAACTTCTCCCTGGCCATCCTGAATGTGGGGGCCCCGGCGGCTGGCATGAATGCGGCCGTGCGCTC GGCGGTGCGGACCGGCATCTCCCATGGACACACAGTATACGTGGTGCACGATGGCTTCGAAGGCCTAGCC AAGGGTCAGGTGCAAGAAGTAGGCTGGCACGACGTGGCCGGCTGGTTGGGGCGTGGTGGCTCCATGCTGG GGACCAAGAGGACCCTGCCCAAGGGCCAGCTGGAGTCCATTGTGGAGAACATCCGCATCTATGGTATTCA CGCCCTGCTGGTGGTCGGTGGGTTTGAGGCCTATGAAGGGGTGCTGCAGCTGGTGGAGGCTCGCGGGCGC TACGAGGAGCTCTGCATCGTCATGTGTGTCATCCCAGCCACCATCAGCAACAACGTCCCTGGCACCGACT TCAGCCTGGGCTCCGACACTGCTGTAAATGCCGCCATGGAGAGCTGTGACCGCATCAAACAGTCTGCCTC GGGGACCAAGCGCCGTGTGTTCATCGTGGAGACCATGGGGGGTTACTGTGGCTACCTGGCCACCGTGACT GGCATTGCTGTGGGGGCCGACGCCGCCTACGTCTTCGAGGACCCTTTCAACATCCACGACTTAAAGGTCA ACGTGGAGCACATGACGGAGAAGATGAAGACAGACATTCAGAGGGGCCTGGTGCTGCGGAACGAGAAGTG CCATGACTACTACACCACGGAGTTCCTGTACAACCTGTACTCATCAGAGGGCAAGGGCGTCTTCGACTGC AGGACCAATGTCCTGGGCCACCTGCAGCAGGGTGGCGCTCCAACCCCCTTTGACCGGAACTATGGGACCA AGCTGGGGGTGAAGGCCATGCTGTGGTTGTCGGAGAAGCTGCGCGAGGTTTACCGCAAGGGACGGGTGTT CGCCAATGCCCCAGACTCGGCCTGCGTGATCGGCCTGAAGAAGAAGGCGGTGGCCTTCAGCCCCGTCACT GAGCTCAAGAAAGACACTGATTTCGAGCACCGCATGCCACGGGAGCAGTGGTGGCTGAGCCTGCGGCTCA TGCTGAAGATGCTGGCACAATACCGCATCAGTATGGCCGCCTACGTGTCAGGGGAGCTGGAGCACGTGAC CCGCCGCACCCTGAGCATGGACAAGGGCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202888 protein sequence
Red=Cloning site Green=Tags(s) MCNQGRGRESSRGGLHVQGSCRGLSRSPQQETGFAKAPAGTDCFFHCSPGSRGQGDRKEEVTSEPGGTSI MSRLGGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKQANWLSVSNIIQLGGTIIGSARCKAFT TREGRRAAAYNLVQHGITNLCVIGGDGSLTGANIFRSEWGSLLEELVAEGKISETTARTYSHLNIAGLVG SIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEA PPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRNGKPISSSYVKDLVVQRLGFDTRVTVLGHVQRGG TPSAFDRILSSKMGMEAVMALLEATPDTPACVVTLSGNQSVRLPLMECVQMTKEVQKAMDDKRFDEATQL RGGSFENNWNIYKLLAHQKPPKEKSNFSLAILNVGAPAAGMNAAVRSAVRTGISHGHTVYVVHDGFEGLA KGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKGQLESIVENIRIYGIHALLVVGGFEAYEGVLQLVEARGR YEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVT GIAVGADAAYVFEDPFNIHDLKVNVEHMTEKMKTDIQRGLVLRNEKCHDYYTTEFLYNLYSSEGKGVFDC RTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWLSEKLREVYRKGRVFANAPDSACVIGLKKKAVAFSPVT ELKKDTDFEHRMPREQWWLSLRLMLKMLAQYRISMAAYVSGELEHVTRRTLSMDKGF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001002021 |
| ORF Size | 2481 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001002021.3 |
| RefSeq Size | 3412 bp |
| RefSeq ORF | 2493 bp |
| Locus ID | 5211 |
| UniProt ID | P17858 |
| Protein Families | Druggable Genome |
| Protein Pathways | Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway |
| MW | 90.2 kDa |
| Gene Summary | This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202888L3 | Lenti ORF clone of Human phosphofructokinase, liver (PFKL), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC202888L4 | Lenti ORF clone of Human phosphofructokinase, liver (PFKL), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG202888 | PFKL (tGFP-tagged) - Human phosphofructokinase, liver (PFKL), transcript variant 1 |
CNY 7,656.00 |
|
| SC125729 | PFKL (untagged)-Human phosphofructokinase, liver (PFKL), transcript variant 1 |
CNY 6,064.00 |
