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GARS1 (NM_002047) Human Tagged ORF Clone
CAT＃: RC202852
GARS (Myc-DDK-tagged)-Human glycyl-tRNA synthetase (GARS)
ORF Plasmid: tGFP
"NM_002047" in other vectors (7)
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CNY 5,800.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CMT2D; DSMAV; GARS; GlyRS; HMN5; HMN5A; SMAD1; SMAJI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202852 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTCTCCGCGTCCAGTGCTGCTTAGAGGTGCTCGCGCCGCTCTGCTGCTGCTGCTGCCGCCCCGGC TCTTAGCCCGACCCTCGCTCCTGCTCCGCCGGTCCCTCAGCGCGGCCTCCTGCGCCCCGATCTCCTTGCC CGCCGCCGCCTCCCGGAGCAGCATGGACGGCGCGGGGGCTGAGGAGGTGCTGGCACCTCTGAGGCTAGCA GTGCGCCAGCAGGGAGATCTTGTGCGAAAACTCAAAGAAGATAAAGCACCCCAAGTAGACGTAGACAAAG CAGTGGCTGAGCTCAAAGCCCGCAAGAGGGTTCTGGAAGCAAAGGAGCTGGCGTTACAGCCCAAAGATGA TATTGTAGACCGAGCAAAAATGGAAGATACCCTGAAGAGGAGGTTTTTCTATGATCAAGCTTTTGCTATT TATGGAGGTGTTAGTGGTCTGTATGACTTTGGGCCAGTTGGCTGTGCTTTGAAGAACAATATTATTCAGA CCTGGAGGCAGCACTTTATCCAAGAGGAACAGATCCTGGAGATCGATTGCACCATGCTCACCCCTGAGCC AGTTTTAAAGACCTCTGGCCATGTAGACAAATTTGCTGACTTCATGGTGAAAGACGTAAAAAATGGAGAA TGTTTTCGTGCTGACCATCTATTAAAAGCTCATTTACAGAAATTGATGTCTGATAAGAAGTGTTCTGTCG AAAAGAAATCAGAAATGGAAAGTGTTTTGGCCCAGCTTGATAACTATGGACAGCAAGAACTTGCGGATCT TTTTGTGAACTATAATGTAAAATCTCCCATTACTGGAAATGATCTATCCCCTCCAGTGTCTTTTAACTTA ATGTTCAAGACTTTCATTGGGCCTGGAGGAAACATGCCTGGGTACTTGAGACCAGAAACTGCACAGGGGA TTTTCTTGAATTTCAAACGACTTTTGGAGTTCAACCAAGGAAAGTTGCCTTTTGCTGCTGCCCAGATTGG AAATTCTTTTAGAAATGAGATCTCCCCTCGATCTGGACTGATCAGAGTCAGAGAATTCACAATGGCAGAA ATTGAGCACTTTGTAGATCCCAGTGAGAAAGACCACCCCAAGTTCCAGAATGTGGCAGACCTTCACCTTT ATTTGTATTCAGCAAAAGCCCAGGTCAGCGGACAGTCCGCTCGGAAAATGCGCCTGGGAGATGCTGTTGA ACAGGGTGTGATTAATAACACAGTATTAGGCTATTTCATTGGCCGCATCTACCTCTACCTCACGAAGGTT GGAATATCTCCAGATAAACTCCGCTTCCGGCAGCACATGGAGAATGAGATGGCCCATTATGCCTGTGACT GTTGGGATGCAGAATCCAAAACATCCTACGGTTGGATTGAGATTGTTGGATGTGCTGATCGTTCCTGTTA TGACCTCTCCTGTCATGCACGAGCCACCAAAGTCCCACTTGTAGCTGAGAAACCTCTGAAAGAACCCAAA ACAGTCAATGTTGTTCAGTTTGAACCCAGTAAGGGAGCAATTGGTAAGGCATATAAGAAGGATGCAAAAC TGGTGATGGAGTATCTTGCCATTTGTGATGAGTGCTACATTACAGAAATGGAGATGCTGCTGAATGAGAA AGGGGAATTCACAATTGAAACTGAAGGGAAAACATTTCAGTTAACAAAAGACATGATCAATGTGAAGAGA TTCCAGAAAACACTATATGTGGAAGAAGTTGTTCCGAATGTAATTGAACCTTCCTTCGGCCTGGGTAGGA TCATGTATACGGTATTTGAACATACATTCCATGTACGAGAAGGAGATGAACAGAGAACATTCTTCAGTTT CCCTGCTGTAGTTGCTCCATTCAAATGTTCCGTCCTCCCACTGAGCCAAAACCAGGAGTTCATGCCATTT GTCAAGGAATTATCGGAAGCCCTGACCAGGCATGGAGTATCTCACAAAGTAGACGATTCCTCTGGGTCAA TCGGAAGGCGCTATGCCAGGACTGATGAGATTGGCGTGGCTTTTGGTGTCACCATTGACTTTGACACAGT GAACAAGACCCCCCACACTGCAACTCTGAGGGACCGTGACTCAATGCGGCAGATAAGAGCAGAGATCTCT GAGCTGCCCAGCATAGTCCAAGACCTAGCCAATGGCAACATCACATGGGCTGATGTGGAGGCCAGGTATC CTCTGTTTGAAGGGCAAGAGACTGGTAAAAAAGAGACAATCGAGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202852 protein sequence
Red=Cloning site Green=Tags(s) MPSPRPVLLRGARAALLLLLPPRLLARPSLLLRRSLSAASCAPISLPAAASRSSMDGAGAEEVLAPLRLA VRQQGDLVRKLKEDKAPQVDVDKAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAI YGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGE CFRADHLLKAHLQKLMSDKKCSVEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNL MFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAE IEHFVDPSEKDHPKFQNVADLHLYLYSAKAQVSGQSARKMRLGDAVEQGVINNTVLGYFIGRIYLYLTKV GISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPK TVNVVQFEPSKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKGEFTIETEGKTFQLTKDMINVKR FQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPF VKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEIS ELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002047 |
| ORF Size | 2217 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002047.4 |
| RefSeq Size | 2759 bp |
| RefSeq ORF | 2220 bp |
| Locus ID | 2617 |
| UniProt ID | P41250 |
| Domains | WHEP-TRS, tRNA-synt_2b, HGTP_anticodon |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
| MW | 83.1 kDa |
| Gene Summary | This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases
,Niehues, S;Bussmann, J;Steffes, G;Erdmann, I;Köhrer, C;Sun, L;Wagner, M;Schäfer, K;Wang, G;Koerdt, SN;Stum, M;RajBhandary, UL;Thomas, U;Aberle, H;Burgess, RW;Yang, XL;Dieterich, D;Storkebaum, E;,
Nat Commun
,PubMed ID 26138142
[GARS1]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202852L1 | Lenti ORF clone of Human glycyl-tRNA synthetase (GARS), Myc-DDK-tagged |
CNY 7,816.00 |
|
| RC202852L2 | Lenti ORF clone of Human glycyl-tRNA synthetase (GARS), mGFP tagged |
CNY 7,700.00 |
|
| RC202852L3 | Lenti ORF clone of Human glycyl-tRNA synthetase (GARS), Myc-DDK-tagged |
CNY 7,816.00 |
|
| RC202852L4 | Lenti ORF clone of Human glycyl-tRNA synthetase (GARS), mGFP tagged |
CNY 7,816.00 |
|
| RG202852 | GARS (tGFP-tagged) - Human glycyl-tRNA synthetase (GARS) |
CNY 7,016.00 |
|
| SC318220 | GARS (untagged)-Human glycyl-tRNA synthetase (GARS) |
CNY 7,220.00 |
|
| SC320151 | GARS (untagged)-Human glycyl-tRNA synthetase (GARS) |
CNY 5,424.00 |
