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SIRT6 (NM_016539) Human Tagged ORF Clone
CAT＃: RC202833
SIRT6 (Myc-DDK-tagged)-Human sirtuin 6 (SIRT6), transcript variant 1
ORF Plasmid: tGFP
"NM_016539" in other vectors (6)
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CNY 5,488.00
| Cited in 6 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SIR2L6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202833 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTGAATTACGCGGCGGGGCTGTCGCCGTACGCGGACAAGGGCAAGTGCGGCCTCCCGGAGATCT TCGACCCCCCGGAGGAGCTGGAGCGGAAGGTGTGGGAACTGGCGAGGCTGGTCTGGCAGTCTTCCAGTGT GGTGTTCCACACGGGTGCCGGCATCAGCACTGCCTCTGGCATCCCCGACTTCAGGGGTCCCCACGGAGTC TGGACCATGGAGGAGCGAGGTCTGGCCCCCAAGTTCGACACCACCTTTGAGAGCGCGCGGCCCACGCAGA CCCACATGGCGCTGGTGCAGCTGGAGCGCGTGGGCCTCCTCCGCTTCCTGGTCAGCCAGAACGTGGACGG GCTCCATGTGCGCTCAGGCTTCCCCAGGGACAAACTGGCAGAGCTCCACGGGAACATGTTTGTGGAAGAA TGTGCCAAGTGTAAGACGCAGTACGTCCGAGACACAGTCGTGGGCACCATGGGCCTGAAGGCCACGGGCC GGCTCTGCACCGTGGCTAAGGCAAGGGGGCTGCGAGCCTGCAGGGGAGAGCTGAGGGACACCATCCTAGA CTGGGAGGACTCCCTGCCCGACCGGGACCTGGCACTCGCCGATGAGGCCAGCAGGAACGCCGACCTGTCC ATCACGCTGGGTACATCGCTGCAGATCCGGCCCAGCGGGAACCTGCCGCTGGCTACCAAGCGCCGGGGAG GCCGCCTGGTCATCGTCAACCTGCAGCCCACCAAGCACGACCGCCATGCTGACCTCCGCATCCATGGCTA CGTTGACGAGGTCATGACCCGGCTCATGAAGCACCTGGGGCTGGAGATCCCCGCCTGGGACGGCCCCCGT GTGCTGGAGAGGGCGCTGCCACCCCTGCCCCGCCCGCCCACCCCCAAGCTGGAGCCCAAGGAGGAATCTC CCACCCGGATCAACGGCTCTATCCCCGCCGGCCCCAAGCAGGAGCCCTGCGCCCAGCACAACGGCTCAGA GCCCGCCAGCCCCAAACGGGAGCGGCCCACCAGCCCTGCCCCCCACAGACCCCCCAAAAGGGTGAAGGCC AAGGCGGTCCCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202833 protein sequence
Red=Cloning site Green=Tags(s) MSVNYAAGLSPYADKGKCGLPEIFDPPEELERKVWELARLVWQSSSVVFHTGAGISTASGIPDFRGPHGV WTMEERGLAPKFDTTFESARPTQTHMALVQLERVGLLRFLVSQNVDGLHVRSGFPRDKLAELHGNMFVEE CAKCKTQYVRDTVVGTMGLKATGRLCTVAKARGLRACRGELRDTILDWEDSLPDRDLALADEASRNADLS ITLGTSLQIRPSGNLPLATKRRGGRLVIVNLQPTKHDRHADLRIHGYVDEVMTRLMKHLGLEIPAWDGPR VLERALPPLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSEPASPKRERPTSPAPHRPPKRVKA KAVPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016539 |
| ORF Size | 1065 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016539.4 |
| RefSeq Size | 1657 bp |
| RefSeq ORF | 1068 bp |
| Locus ID | 51548 |
| UniProt ID | Q8N6T7 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 39.1 kDa |
| Gene Summary | This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016] |
Citations (6)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The SIRT6-Autophagy-Warburg Effect Axis in Papillary Thyroid Cancer
,null,
Frontiers in Oncology
,PubMed ID 32983963
[SIRT6]
|
|
NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma
,Zhou, HZ;Zeng, HQ;Yuan, D;Ren, JH;Cheng, ST;Yu, HB;Ren, F;Wang, Q;Qin, YP;Huang, AL;Chen, J;,
Cell Commun. Signal
,PubMed ID 31842909
[SIRT6]
|
|
microRNA-4532 inhibition protects human lens epithelial cells from ultra-violet-induced oxidative injury via activating SIRT6-Nrf2 signaling
,Sun, GL;Huang, D;Li, KR;Jiang, Q;,
Biochem. Biophys. Res. Commun.
,PubMed ID 31079921
[SIRT6]
|
|
SIRT6 promotes the Warburg effect of papillary thyroid cancer cell BCPAP through reactive oxygen species
,Yu, W;Yang, Z;Huang, R;Min, Z;Ye, M;,
Onco Targets Ther
,PubMed ID 31114231
[SIRT6]
|
|
SIRT6/HIF-1α axis promotes papillary thyroid cancer progression by inducing epithelial–mesenchymal transition
,null,
Cancer Cell International
,PubMed ID 30675128
[SIRT6]
|
|
SIRT6 Depletion Sensitizes Human Hepatoma Cells to Chemotherapeutics by Downregulating MDR1 Expression
,Xia, YQ;Hua, RJ;Juan, C;Zhong, ZH;Tao, CS;Fang, R;Lin, H;Rui, G;Yong, C;,
Front Pharmacol
,PubMed ID 29563873
[SIRT6]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202833L1 | Lenti ORF clone of Human sirtuin 6 (SIRT6), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC202833L2 | Lenti ORF clone of Human sirtuin 6 (SIRT6), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RC202833L3 | Lenti ORF clone of Human sirtuin 6 (SIRT6), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202833L4 | Lenti ORF clone of Human sirtuin 6 (SIRT6), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG202833 | SIRT6 (tGFP-tagged) - Human sirtuin 6 (SIRT6), transcript variant 1 |
CNY 7,088.00 |
|
| SC123758 | SIRT6 (untagged)-Human sirtuin 6 (SIRT6), transcript variant 1 |
CNY 3,656.00 |
