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PTEN (NM_000314) Human Tagged ORF Clone
CAT＃: RC202627
PTEN (Myc-DDK-tagged)-Human phosphatase and tensin homolog (PTEN)
ORF Plasmid: tGFP
"NM_000314" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 14 publications. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; PTENbeta; TEP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202627 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCCATCATCAAAGAGATCGTTAGCAGAAACAAAAGGAGATATCAAGAGGATGGATTCGACTTAG ACTTGACCTATATTTATCCAAACATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAAGGCGTATACAG GAACAATATTGATGATGTAGTAAGGTTTTTGGATTCAAAGCATAAAAACCATTACAAGATATACAATCTT TGTGCTGAAAGACATTATGACACCGCCAAATTTAATTGCAGAGTTGCACAATATCCTTTTGAAGACCATA ACCCACCACAGCTAGAACTTATCAAACCCTTTTGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAA TCATGTTGCAGCAATTCACTGTAAAGCTGGAAAGGGACGAACTGGTGTAATGATATGTGCATATTTATTA CATCGGGGCAAATTTTTAAAGGCACAAGAGGCCCTAGATTTCTATGGGGAAGTAAGGACCAGAGACAAAA AGGGAGTAACTATTCCCAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATCTGGA TTATAGACCAGTGGCACTGTTGTTTCACAAGATGATGTTTGAAACTATTCCAATGTTCAGTGGCGGAACT TGCAATCCTCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCACACGAC GGGAAGACAAGTTCATGTACTTTGAGTTCCCTCAGCCGTTACCTGTGTGTGGTGATATCAAAGTAGAGTT CTTCCACAAACAGAACAAGATGCTAAAAAAGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATA CCAGGACCAGAGGAAACCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGCATTT GCAGTATAGAGCGTGCAGATAATGACAAGGAATATCTAGTACTTACTTTAACAAAAAATGATCTTGACAA AGCAAATAAAGACAAAGCCAACCGATACTTTTCTCCAAATTTTAAGGTGAAGCTGTACTTCACAAAAACA GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACCAGATGTTAGTGACAATGAAC CTGATCATTATAGATATTCTGACACCACTGACTCTGATCCAGAGAATGAACCTTTTGATGAAGATCAGCA TACACAAATTACAAAAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202627 protein sequence
Red=Cloning site Green=Tags(s) MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNL CAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLL HRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGT CNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFI PGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKT VEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000314 |
| ORF Size | 1209 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000314.7 |
| RefSeq Size | 5572 bp |
| RefSeq ORF | 1212 bp |
| Locus ID | 5728 |
| UniProt ID | P60484 |
| Domains | PTPc_motif |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Endometrial cancer, Focal adhesion, Glioma, Inositol phosphate metabolism, Melanoma, p53 signaling pathway, Pathways in cancer, Phosphatidylinositol signaling system, Prostate cancer, Small cell lung cancer, Tight junction |
| MW | 47.2 kDa |
| Gene Summary | This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015] |
Citations (14)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation
,Yang, Z;Shi, J;Xie, J;Wang, Y;Sun, J;Liu, T;Zhao, Y;Zhao, X;Wang, X;Ma, Y;Malkoc, V;Chiang, C;Deng, W;Chen, Y;Fu, Y;Kwak, KJ;Fan, Y;Kang, C;Yin, C;Rhee, J;Bertani, P;Otero, J;Lu, W;Yun, K;Lee, AS;Jiang, W;Teng, L;Kim, BYS;Lee, LJ;,
Nat Biomed Eng
,PubMed ID 31844155
[PTEN]
|
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Downregulation of miR-103a-3p contributes to endothelial progenitor cell dysfunction in deep vein thrombosis through PTEN targeting
,Zhang, P;Zhao, Q;Gong, K;Long, Y;Zhang, J;Li, Y;Guo, X;,
Ann Vasc Surg
,PubMed ID 31639479
[PTEN]
|
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PTEN-induced partial epithelial-mesenchymal transition drives diabetic kidney disease
,Li, Y;Hu, Q;Li, C;Liang, K;Xiang, Y;Hsiao, H;Nguyen, TK;Park, PK;Egranov, SD;Ambati, CR;Putluri, N;Hawke, DH;Han, L;Hung, MC;Danesh, FR;Yang, L;Lin, C;,
J. Clin. Invest.
,PubMed ID 30741721
[PTEN]
|
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Down-regulation of miR-543 expression increases the sensitivity of colorectal cancer cells to 5-Fluorouracil through the PTEN/PI3K/AKT pathway
,Liu, G;Zhou, J;Dong, M;,
Biosci. Rep.
,PubMed ID 30842340
[PTEN]
|
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Long noncoding RNA MEG3 suppresses liver cancer cells growth through inhibiting β-catenin by activating PKM2 and inactivating PTEN
,Zheng, Q;Lin, Z;Xu, J;Lu, Y;Meng, Q;Wang, C;Yang, Y;Xin, X;Li, X;Pu, H;Gui, X;Li, T;Xiong, W;Lu, D;,
Cell Death Dis
,PubMed ID 29449541
[PTEN]
|
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Rapid Loss of RNA Detection by In Situ Hybridization in Stored Tissue Blocks and Preservation by Cold Storage of Unstained Slides
,Baena-Del Valle, JA;Zheng, Q;Hicks, JL;Fedor, H;Trock, BJ;Morrissey, C;Corey, E;Cornish, TC;Sfanos, KS;De Marzo, AM;,
Am. J. Clin. Pathol.
,PubMed ID 29106457
[PTEN]
|
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Hypoxic Lung-Cancer-Derived Extracellular Vesicle MicroRNA-103a Increases the Oncogenic Effects of Macrophages by Targeting PTEN
,Hsu, YL;Hung, JY;Chang, WA;Jian, SF;Lin, YS;Pan, YC;Wu, CY;Kuo, PL;,
Mol. Ther.
,PubMed ID 29292163
[PTEN]
|
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Phosphatidylinositol-3 kinase-dependent translational regulation of Id1 involves the PPM1G phosphatase
,Xu, K;Wang, L;Feng, W;Feng, Y;Shu, HK;,
Oncogene
,PubMed ID 27065332
[PTEN]
|
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Neuronal RARß Signaling Modulates PTEN Activity Directly in Neurons and via Exosome Transfer in Astrocytes to Prevent Glial Scar Formation and Induce Spinal Cord Regeneration
,Goncalves, MB;Malmqvist, T;Clarke, E;Hubens, CJ;Grist, J;Hobbs, C;Trigo, D;Risling, M;Angeria, M;Damberg, P;Carlstedt, TP;Corcoran, JP;,
J. Neurosci.
,PubMed ID 26609164
[PTEN]
|
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Effects of PTEN on the longevity of cultured human umbilical vein endothelial cells: The role of antioxidants
,Tait, IS;Li, Y;Lu, J;,
Int. J. Mol. Med.
,PubMed ID 25395086
[PTEN]
|
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Die Rolle des Tumorsuppressors PTEN bei der molekularen Pathogenese diffuser großzelliger B-Zell-Lymphome
,Pfeifer, M;,
Thesis
[PTEN]
|
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The effects of Phosphatase and Tensin Homolog (PTEN) overexpression on longevity of cultured Human Umbilical Vein Endothelial Cells (HUVEC)
,Tait, IS;,
Thesis
[PTEN]
|
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HER2 overcomes PTEN (loss)-induced senescence to cause aggressive prostate cancer
,Imran Ahmad, Rachana Patel, Lukram Babloo Singh, Colin Nixon, Morag Seywright, Robert J. Barnetson, Valerie G. Brunton, William J. Muller, Joanne Edwards, Owen J. Sansom, and Hing Y. Leung,
PNAS, Sep 2011; 108: 16392 - 16397.
[PTEN]
|
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PTEN Protein Loss by Immunostaining: Analytic Validation and Prognostic Indicator for a High Risk Surgical Cohort of Prostate Cancer Patients
,Tamara L. Lotan, Bora Gurel, Siobhan Sutcliffe, David Esopi, Wennuan Liu, Jianfeng Xu, Jessica L. Hicks, Ben H. Park, Elizabeth Humphreys, Alan W. Partin, Misop Han, George J. Netto, William B. Isaacs, and Angelo M. De Marzo,
Clin. Cancer Res., Oct 2011; 17: 6563 - 6573.
[PTEN]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202627L1 | Lenti ORF clone of Human phosphatase and tensin homolog (PTEN), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC202627L2 | Lenti ORF clone of Human phosphatase and tensin homolog (PTEN), mGFP tagged |
CNY 6,056.00 |
|
| RC202627L3 | Lenti ORF clone of Human phosphatase and tensin homolog (PTEN), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC202627L4 | Lenti ORF clone of Human phosphatase and tensin homolog (PTEN), mGFP tagged |
CNY 6,056.00 |
|
| RG202627 | PTEN (tGFP-tagged) - Human phosphatase and tensin homolog (PTEN) |
CNY 5,256.00 |
|
| SC119965 | PTEN (untagged)-Human phosphatase and tensin homolog (PTEN) |
CNY 5,488.00 |
