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Malectin (MLEC) (NM_014730) Human Tagged ORF Clone
CAT＃: RC202607
MLEC (Myc-DDK-tagged)-Human malectin (MLEC)
ORF Plasmid: tGFP
"NM_014730" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KIAA0152 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202607 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGAGCCTGGGCGGTTGAGGGAACCGCTGTGGCGCTCCTGCGACTGCTGCTGCTGCTGCTGCCGC CGGCGATCCGGGGACCCGGGCTCGGCGTGGCCGGCGTGGCCGGCGCGGCGGGGGCCGGGCTGCCCGAGAG CGTCATTTGGGCGGTCAACGCGGGTGGAGAGGCGCATGTGGACGTGCACGGGATCCACTTCCGCAAGGAC CCTTTGGAAGGCCGGGTGGGCCGAGCCTCAGACTATGGCATGAAACTGCCAATCCTGCGTTCCAACCCTG AGGACCAGATCCTGTATCAAACTGAGCGGTACAATGAGGAGACCTTTGGCTACGAAGTGCCCATCAAAGA GGAGGGGGACTACGTGCTGGTCTTGAAATTTGCAGAGGTCTACTTTGCACAGTCCCAGCAAAAGGTATTT GATGTACGATTGAATGGCCACGTCGTGGTGAAGGACTTGGATATCTTTGATCGTGTTGGGCATAGCACAG CTCACGATGAAATTATACCTATGAGCATCAGAAAGGGGAAGCTGAGTGTCCAGGGGGAGGTGTCCACCTT CACAGGGAAACTCTACATTGAGTTTGTCAAGGGGTACTATGACAATCCCAAGGTCTGTGCACTCTACATC ATGGCTGGGACAGTGGATGATGTACCAAAGCTTCAGCCTCATCCGGGATTGGAGAAGAAAGAAGAGGAAG AAGAAGAAGAAGAATATGATGAAGGGTCTAATCTCAAAAAACAGACCAATAAGAACCGGGTGCAGTCAGG CCCCCGCACACCCAACCCCTATGCCTCGGACAACAGCAGCCTCATGTTTCCCATCCTGGTGGCCTTCGGA GTCTTCATTCCAACCCTCTTCTGCCTCTGCCGGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202607 protein sequence
Red=Cloning site Green=Tags(s) MLGAWAVEGTAVALLRLLLLLLPPAIRGPGLGVAGVAGAAGAGLPESVIWAVNAGGEAHVDVHGIHFRKD PLEGRVGRASDYGMKLPILRSNPEDQILYQTERYNEETFGYEVPIKEEGDYVLVLKFAEVYFAQSQQKVF DVRLNGHVVVKDLDIFDRVGHSTAHDEIIPMSIRKGKLSVQGEVSTFTGKLYIEFVKGYYDNPKVCALYI MAGTVDDVPKLQPHPGLEKKEEEEEEEEYDEGSNLKKQTNKNRVQSGPRTPNPYASDNSSLMFPILVAFG VFIPTLFCLCRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014730 |
| ORF Size | 876 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014730.4 |
| RefSeq Size | 6367 bp |
| RefSeq ORF | 879 bp |
| Locus ID | 9761 |
| UniProt ID | Q14165 |
| Protein Families | Transmembrane |
| MW | 32.2 kDa |
| Gene Summary | This gene encodes the carbohydrate-binding protein malectin which is a Type I membrane-anchored endoplasmic reticulum protein. This protein has an affinity for Glc2Man9GlcNAc2 (G2M9) N-glycans and is involved in regulating glycosylation in the endoplasmic reticulum. This protein has also been shown to interact with ribophorin I and may be involved in the directing the degradation of misfolded proteins. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202607L3 | Lenti ORF clone of Human malectin (MLEC), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202607L4 | Lenti ORF clone of Human malectin (MLEC), mGFP tagged |
CNY 4,800.00 |
|
| RG202607 | MLEC (tGFP-tagged) - Human malectin (MLEC) |
CNY 4,370.00 |
|
| SC319235 | MLEC (untagged)-Human malectin (MLEC) |
CNY 2,400.00 |
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