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MPP8 (MPHOSPH8) (NM_017520) Human Tagged ORF Clone
CAT＃: RC202562
MPHOSPH8 (Myc-DDK-tagged)-Human M-phase phosphoprotein 8 (MPHOSPH8)
ORF Plasmid: tGFP
"NM_017520" in other vectors (4)
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CNY 6,296.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HSMPP8; mpp8; TWA3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202562 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGGTTGCGGAGGGAGCAAGGGTGACCGCAGTCCCTGTGTCAGCTGCCGACAGCACTGAGGAGT TGGCCGAAGTCGAAGAAGGAGTTGGAGTAGTGGGCGAAGATAATGACGCAGCCGCGAGAGGAGCGGAGGC CTTTGGCGACAGTGAGGAGGACGGAGAGGATGTGTTCGAGGTGGAGAAGATCCTGGACATGAAGACCGAG GGGGGTAAAGTTCTTTACAAAGTTCGCTGGAAAGGCTATACATCGGATGATGATACCTGGGAGCCCGAGA TTCACCTGGAGGACTGTAAAGAAGTGCTTCTTGAATTTAGGAAGAAAATTGCAGAGAACAAAGCCAAAGC AGTCAGGAAGGATATTCAGAGACTATCCTTAAATAACGACATATTTGAGGCGAACTCTGATAGCGATCAG CAAAGTGAGACAAAAGAAGATACTTCCCCAAAGAAGAAAAAGAAAAAATTGAGGCAGAGAGAAGAGAAAA GCCCAGATGATCTGAAAAAGAAAAAAGCAAAGGCCGGGAAGCTAAAAGACAAGTCCAAACCAGACCTGGA GAGCTCCTTGGAAAGTTTAGTTTTTGATTTAAGGACAAAGAAAAGAATTTCTGAAGCCAAAGAAGAACTA AAGGAGTCCAAAAAGCCCAAAAAAGATGAAGTAAAAGAAACAAAAGAATTAAAGAAAGTTAAAAAGGGTG AAATAAGAGATTTAAAGACGAAAACAAGAGAAGATCCCAAAGAAAATAGAAAAACAAAAAAAGAAAAATT TGTCGAATCCCAGGTGGAATCTGAATCAAGTGTACTTAATGATTCTCCCTTTCCAGAGGATGACAGTGAA GGGCTACATTCCGACAGCAGAGAAGAGAAACAAAACACTAAAAGTGCAAGAGAGAGAGCAGGGCAGGACA TGGGGCTGGAGCATGGCTTTGAGAAGCCCCTAGACAGTGCCATGAGTGCTGAGGAGGATACCGATGTCAG AGGCAGGAGGAAAAAGAAGACCCCGAGAAAGGCTGAGGACACTAGAGAGAACAGGAAGCTAGAGAACAAG AACGCTTTCTTAGAGAAGAAAACTGTGCCTAAAAAGCAGAGGAATCAAGACAGAAGCAAAAGTGCTGCAG AGTTAGAGAAGCTGATGCCTGTATCTGCCCAAACGCCAAAGGGCCGGAGGTTGAGCGGGGAAGAGAGAGG CCTCTGGTCCACGGACTCAGCCGAGGAGGACAAAGAAACCAAAAGAAATGAATCCAAAGAAAAATATCAG AAAAGGCATGATTCTGACAAGGAAGAAAAAGGCAGAAAAGAGCCAAAAGGATTAAAGACACTTAAGGAAA TCAGAAATGCATTTGATTTATTTAAATTAACTCCAGAAGAAAAAAATGATGTTTCTGAGAATAATCGGAA AAGGGAAGAAATACCACTGGATTTTAAAACCATAGACGATCACAAAACCAAGGAAAACAAACAGTCACTT AAAGAAAGGAGAAACACCAGAGACGAAACGGATACTTGGGCATACATTGCTGCAGAAGGTGATCAGGAGG TTTTAGACAGCGTGTGCCAAGCAGATGAGAATTCAGATGGCAGGCAGCAGATTCTGAGTTTGGGCATGGA CCTGCAGTTGGAATGGATGAAGTTGGAAGATTTCCAAAAGCACCTTGATGGGAAAGATGAGAATTTTGCT GCAACAGATGCAATTCCAAGTAATGTGTTAAGGGATGCTGTGAAAAATGGGGATTATATTACTGTAAAAG TTGCACTTAATTCAAATGAAGAATATAACCTGGACCAAGAGGATTCCAGTGGAATGACACTGGTGATGCT TGCCGCCGCCGGAGGGCAGGACGACCTCCTGCGACTCCTCATCACAAAAGGCGCGAAAGTGAACGGTCGG CAGAAGAACGGGACCACCGCCCTCATTCATGCTGCAGAGAAGAACTTTTTAACAACAGTGGCTATTCTTT TGGAAGCAGGAGCTTTTGTAAATGTCCAGCAAAGCAATGGTGAGACTGCACTGATGAAGGCCTGTAAAAG AGGAAATTCAGACATCGTACGACTCGTAATTGAATGTGGAGCTGACTGCAATATTTTGTCAAAGCACCAG AATAGTGCCCTGCACTTTGCGAAGCAGTCTAACAATGTGCTTGTGTACGACTTGCTGAAGAACCATTTAG AGACACTTTCAAGAGTAGCAGAAGAGACAATAAAGGATTACTTTGAAGCTCGCCTTGCTCTGCTAGAACC AGTTTTTCCAATCGCATGTCATCGACTCTGTGAGGGTCCAGATTTTTCAACAGATTTCAATTACAAACCC CCACAGAACATACCAGAAGGCTCTGGCATCCTGCTGTTTATCTTCCATGCAAACTTTTTGGGTAAAGAAG TTATTGCTCGGCTCTGTGGACCGTGTAGTGTACAAGCTGTAGTTCTGAATGATAAATTTCAGCTTCCTGT TTTTCTGGACAGTCATTTTGTTTACTCATTCAGCCCTGTTGCAGGTCCCAATAAACTCTTCATAAGGTTG ACAGAAGCACCCTCTGCCAAGGTTAAGTTGCTAATAGGTGCATACAGAGTGCAGCTGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202562 protein sequence
Red=Cloning site Green=Tags(s) MEQVAEGARVTAVPVSAADSTEELAEVEEGVGVVGEDNDAAARGAEAFGDSEEDGEDVFEVEKILDMKTE GGKVLYKVRWKGYTSDDDTWEPEIHLEDCKEVLLEFRKKIAENKAKAVRKDIQRLSLNNDIFEANSDSDQ QSETKEDTSPKKKKKKLRQREEKSPDDLKKKKAKAGKLKDKSKPDLESSLESLVFDLRTKKRISEAKEEL KESKKPKKDEVKETKELKKVKKGEIRDLKTKTREDPKENRKTKKEKFVESQVESESSVLNDSPFPEDDSE GLHSDSREEKQNTKSARERAGQDMGLEHGFEKPLDSAMSAEEDTDVRGRRKKKTPRKAEDTRENRKLENK NAFLEKKTVPKKQRNQDRSKSAAELEKLMPVSAQTPKGRRLSGEERGLWSTDSAEEDKETKRNESKEKYQ KRHDSDKEEKGRKEPKGLKTLKEIRNAFDLFKLTPEEKNDVSENNRKREEIPLDFKTIDDHKTKENKQSL KERRNTRDETDTWAYIAAEGDQEVLDSVCQADENSDGRQQILSLGMDLQLEWMKLEDFQKHLDGKDENFA ATDAIPSNVLRDAVKNGDYITVKVALNSNEEYNLDQEDSSGMTLVMLAAAGGQDDLLRLLITKGAKVNGR QKNGTTALIHAAEKNFLTTVAILLEAGAFVNVQQSNGETALMKACKRGNSDIVRLVIECGADCNILSKHQ NSALHFAKQSNNVLVYDLLKNHLETLSRVAEETIKDYFEARLALLEPVFPIACHRLCEGPDFSTDFNYKP PQNIPEGSGILLFIFHANFLGKEVIARLCGPCSVQAVVLNDKFQLPVFLDSHFVYSFSPVAGPNKLFIRL TEAPSAKVKLLIGAYRVQLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017520 |
| ORF Size | 2580 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017520.4 |
| RefSeq Size | 4281 bp |
| RefSeq ORF | 2583 bp |
| Locus ID | 54737 |
| UniProt ID | Q99549 |
| MW | 97.2 kDa |
| Gene Summary | Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed:26022416, PubMed:28581500). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202562L3 | Lenti ORF clone of Human M-phase phosphoprotein 8 (MPHOSPH8), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC202562L4 | Lenti ORF clone of Human M-phase phosphoprotein 8 (MPHOSPH8), mGFP tagged |
CNY 8,696.00 |
|
| RG202562 | MPHOSPH8 (tGFP-tagged) - Human M-phase phosphoprotein 8 (MPHOSPH8) |
CNY 7,896.00 |
|
| SC304472 | MPHOSPH8 (untagged)-Human M-phase phosphoprotein 8 (MPHOSPH8) |
CNY 16,630.00 |
