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GPSN2 (TECR) (NM_138501) Human Tagged ORF Clone
CAT＃: RC202503
TECR (Myc-DDK-tagged)-Human trans-2,3-enoyl-CoA reductase (TECR), transcript variant 1
ORF Plasmid: tGFP
"NM_138501" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GPSN2; MRT14; SC2; TER |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202503 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCATTACGAGGTGGAGATTCTGGACGCAAAGACAAGGGAGAAGCTGTGTTTCTTGGACAAGGTGG AGCCCCACGCCACCATTGCGGAGATCAAGAACCTCTTCACTAAGACCCATCCGCAGTGGTACCCCGCCCG CCAGTCCCTCCGCCTGGACCCCAAGGGCAAGTCCCTGAAGGATGAGGATGTTCTGCAGAAGCTGCCCGTG GGCACCACGGCCACACTGTACTTCCGGGACCTGGGGGCCCAGATCAGCTGGGTGACGGTCTTCCTAACAG AGTACGCGGGGCCCCTTTTCATCTACCTGCTCTTCTACTTCCGAGTGCCCTTCATCTATGGCCACAAATA TGACTTTACGTCCAGTCGGCATACAGTGGTGCACCTCGCCTGCATCTGTCACTCATTCCACTACATCAAG CGCCTGCTGGAGACGCTCTTCGTGCACCGCTTCTCCCATGGCACTATGCCTTTGCGCAACATCTTCAAGA ACTGCACCTACTACTGGGGCTTCGCCGCGTGGATGGCCTATTACATCAATCACCCTCTCTACACTCCCCC TACCTACGGAGCTCAGCAGGTGAAACTGGCGCTCGCCATCTTTGTGATCTGCCAGCTCGGCAACTTCTCC ATCCACATGGCCCTGCGGGACCTGCGGCCCGCTGGGTCCAAGACGCGGAAGATCCCATACCCCACCAAGA ACCCCTTCACGTGGCTCTTCCTGCTGGTGTCCTGCCCCAACTACACCTACGAGGTGGGGTCCTGGATCGG TTTCGCCATCATGACGCAGTGTCTCCCAGTGGCCCTGTTCTCCCTGGTGGGCTTCACCCAGATGACCATC TGGGCCAAGGGCAAGCACCGCAGCTACCTGAAGGAGTTCCGGGACTACCCGCCCCTGCGCATGCCCATCA TCCCCTTCCTGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202503 protein sequence
Red=Cloning site Green=Tags(s) MKHYEVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGHKYDFTSSRHTVVHLACICHSFHYIK RLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFS IHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTI WAKGKHRSYLKEFRDYPPLRMPIIPFLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138501 |
| ORF Size | 924 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138501.6 |
| RefSeq Size | 1180 bp |
| RefSeq ORF | 927 bp |
| Locus ID | 9524 |
| UniProt ID | Q9NZ01 |
| Domains | Steroid_dh |
| Protein Families | Transmembrane |
| Protein Pathways | Biosynthesis of unsaturated fatty acids |
| MW | 36 kDa |
| Gene Summary | This gene encodes a multi-pass membrane protein that resides in the endoplasmic reticulum, and belongs to the steroid 5-alpha reductase family. The elongation of microsomal long and very long chain fatty acid consists of 4 sequential reactions. This protein catalyzes the final step, reducing trans-2,3-enoyl-CoA to saturated acyl-CoA. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Apr 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202503L3 | Lenti ORF clone of Human trans-2,3-enoyl-CoA reductase (TECR), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202503L4 | Lenti ORF clone of Human trans-2,3-enoyl-CoA reductase (TECR), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG202503 | TECR (tGFP-tagged) - Human trans-2,3-enoyl-CoA reductase (TECR), transcript variant 1 |
CNY 4,000.00 |
|
| SC319230 | TECR (untagged)-Human trans-2,3-enoyl-CoA reductase (TECR), transcript variant 1 |
CNY 2,400.00 |
