United States
Germany
Japan
United Kingdom
China
ADFP (PLIN2) (NM_001122) Human Tagged ORF Clone
CAT＃: RC202500
PLIN2 (Myc-DDK-tagged)-Human perilipin 2 (PLIN2), transcript variant 1
ORF Plasmid: tGFP
"NM_001122" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADFP; ADRP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202500 representing NM_001122
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCCGTTGCAGTTGATCCACAACCGAGTGTGGTGACTCGGGTGGTCAACCTGCCCTTGGTGAGCT CCACGTATGACCTCATGTCCTCAGCCTATCTCAGTACAAAGGACCAGTATCCCTACCTGAAGTCTGTGTG TGAGATGGCAGAGAACGGTGTGAAGACCATCACCTCCGTGGCCATGACCAGTGCTCTGCCCATCATCCAG AAGCTAGAGCCGCAAATTGCAGTTGCCAATACCTATGCCTGTAAGGGGCTAGACAGGATTGAGGAGAGAC TGCCTATTCTGAATCAGCCATCAACTCAGATTGTTGCCAATGCCAAAGGCGCTGTGACTGGGGCAAAAGA TGCTGTGACGACTACTGTGACTGGGGCCAAGGATTCTGTGGCCAGCACGATCACAGGGGTGATGGACAAG ACCAAAGGGGCAGTGACTGGCAGTGTGGAGAAGACCAAGTCTGTGGTCAGTGGCAGCATTAACACAGTCT TGGGGAGTCGGATGATGCAGCTCGTGAGCAGTGGCGTAGAAAATGCACTCACCAAATCAGAGCTGTTGGT AGAACAGTACCTCCCTCTCACTGAGGAAGAACTAGAAAAAGAAGCAAAAAAAGTTGAAGGATTTGATCTG GTTCAGAAGCCAAGTTATTATGTTAGACTGGGATCCCTGTCTACCAAGCTTCACTCCCGTGCCTACCAGC AGGCTCTCAGCAGGGTTAAAGAAGCTAAGCAAAAAAGCCAACAGACCATTTCTCAGCTCCATTCTACTGT TCACCTGATTGAATTTGCCAGGAAGAATGTGTATAGTGCCAATCAGAAAATTCAGGATGCTCAGGATAAG CTCTACCTCTCATGGGTAGAGTGGAAAAGGAGCATTGGATATGATGATACTGATGAGTCCCACTGTGCTG AGCACATTGAGTCACGTACTCTTGCAATTGCCCGCAACCTGACTCAGCAGCTCCAGACCACGTGCCACAC CCTCCTGTCCAACATCCAAGGTGTACCACAGAACATCCAAGATCAAGCCAAGCACATGGGGGTGATGGCA GGCGACATCTACTCAGTGTTCCGCAATGCTGCCTCCTTTAAAGAAGTGTCTGACAGCCTCCTCACTTCTA GCAAGGGGCAGCTGCAGAAAATGAAGGAATCTTTAGATGACGTGATGGATTATCTTGTTAACAACACGCC CCTCAACTGGCTGGTAGGTCCCTTTTATCCTCAGCTGACTGAGTCTCAGAATGCTCAGGACCAAGGTGCA GAGATGGACAAGAGCAGCCAGGAGACCCAGCGATCTGAGCATAAAACTCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202500 representing NM_001122
Red=Cloning site Green=Tags(s) MASVAVDPQPSVVTRVVNLPLVSSTYDLMSSAYLSTKDQYPYLKSVCEMAENGVKTITSVAMTSALPIIQ KLEPQIAVANTYACKGLDRIEERLPILNQPSTQIVANAKGAVTGAKDAVTTTVTGAKDSVASTITGVMDK TKGAVTGSVEKTKSVVSGSINTVLGSRMMQLVSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDL VQKPSYYVRLGSLSTKLHSRAYQQALSRVKEAKQKSQQTISQLHSTVHLIEFARKNVYSANQKIQDAQDK LYLSWVEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGVPQNIQDQAKHMGVMA GDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESLDDVMDYLVNNTPLNWLVGPFYPQLTESQNAQDQGA EMDKSSQETQRSEHKTH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001122 |
| ORF Size | 1311 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001122.4 |
| RefSeq Size | 2010 bp |
| RefSeq ORF | 1314 bp |
| Locus ID | 123 |
| UniProt ID | Q99541 |
| Domains | perilipin |
| Protein Families | Druggable Genome |
| MW | 47.9 kDa |
| Gene Summary | The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202500L1 | Lenti ORF clone of Human perilipin 2 (PLIN2), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC202500L2 | Lenti ORF clone of Human perilipin 2 (PLIN2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC202500L3 | Lenti ORF clone of Human perilipin 2 (PLIN2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202500L4 | Lenti ORF clone of Human perilipin 2 (PLIN2), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG202500 | PLIN2 (tGFP-tagged) - Human perilipin 2 (PLIN2), transcript variant 1 |
CNY 5,256.00 |
|
| SC127716 | PLIN2 (untagged)-Human perilipin 2 (PLIN2), transcript variant 1 |
CNY 3,656.00 |
