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GM2A (NM_000405) Human Tagged ORF Clone
CAT＃: RC202452
GM2A (Myc-DDK-tagged)-Human GM2 ganglioside activator (GM2A), transcript variant 1
ORF Plasmid: tGFP
"NM_000405" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GM2-AP; SAP-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202452 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTCCCTGATGCAGGCTCCCCTCCTGATCGCCCTGGGCTTGCTTCTCGCGGCCCCTGCGCAAGCCC ACCTGAAAAAGCCATCCCAGCTCAGTAGCTTTTCCTGGGATAACTGTGATGAAGGGAAGGACCCTGCGGT GATCAGAAGCCTGACTCTGGAGCCTGACCCCATCGTCGTTCCTGGAAATGTGACCCTCAGTGTCGTGGGC AGCACCAGTGTCCCCCTGAGTTCTCCTCTGAAGGTGGATTTAGTTTTGGAGAAGGAGGTGGCTGGCCTCT GGATCAAGATCCCATGCACAGACTACATTGGCAGCTGTACCTTTGAACACTTCTGTGATGTGCTTGACAT GTTAATTCCTACTGGGGAGCCCTGCCCAGAGCCCCTGCGTACCTATGGGCTTCCTTGCCACTGTCCCTTC AAAGAAGGAACCTACTCACTGCCCAAGAGCGAATTCGTTGTGCCTGACCTGGAGCTGCCCAGTTGGCTCA CCACCGGGAACTACCGCATAGAGAGCGTCCTGAGCAGCAGTGGGAAGCGTCTGGGCTGCATCAAGATCGC TGCCTCTCTAAAGGGCATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202452 protein sequence
Red=Cloning site Green=Tags(s) MQSLMQAPLLIALGLLLAAPAQAHLKKPSQLSSFSWDNCDEGKDPAVIRSLTLEPDPIVVPGNVTLSVVG STSVPLSSPLKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPF KEGTYSLPKSEFVVPDLELPSWLTTGNYRIESVLSSSGKRLGCIKIAASLKGI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000405 |
| ORF Size | 579 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000405.5 |
| RefSeq Size | 3690 bp |
| RefSeq ORF | 582 bp |
| Locus ID | 2760 |
| UniProt ID | P17900 |
| Domains | ML |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysosome |
| MW | 20.8 kDa |
| Gene Summary | This gene encodes a small glycolipid transport protein which acts as a substrate specific co-factor for the lysosomal enzyme beta-hexosaminidase A. Beta-hexosaminidase A, together with GM2 ganglioside activator, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene result in GM2-gangliosidosis type AB or the AB variant of Tay-Sachs disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Membrane lipids regulate ganglioside GM2 catabolism and GM2 activator protein activity
,Anheuser, S;Breiden, B;Schwarzmann, G;Sandhoff, K;,
J. Lipid Res.
,PubMed ID 26175473
[GM2A]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202452L1 | Lenti ORF clone of Human GM2 ganglioside activator (GM2A), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC202452L2 | Lenti ORF clone of Human GM2 ganglioside activator (GM2A), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RC202452L3 | Lenti ORF clone of Human GM2 ganglioside activator (GM2A), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202452L4 | Lenti ORF clone of Human GM2 ganglioside activator (GM2A), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG202452 | GM2A (tGFP-tagged) - Human GM2 ganglioside activator (GM2A), transcript variant 1 |
CNY 4,000.00 |
|
| SC126904 | GM2A (untagged)-Human GM2 ganglioside activator (GM2A), transcript variant 1 |
CNY 2,400.00 |
