FANCG (NM_004629) Human Tagged ORF Clone

CAT#: RC202443

FANCG (Myc-DDK-tagged)-Human Fanconi anemia, complementation group G (FANCG)

ORF Plasmid: DDK tGFP



  "NM_004629" in other vectors (6)

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CNY 4,632.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Goat Polyclonal Antibody against FANCG / XRCC9
    • 100 ug

CNY 5,371.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms FAG; XRCC9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202443 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCGCCAGACCACCTCTGTGGGCTCCAGCTGCCTGGACCTGTGGAGGGAAAAGAATGACCGGCTCG
TTCGACAGGCCAAGGTGGCTCAGAACTCCGGTCTGACTCTGAGGCGACAGCAGTTGGCTCAGGATGCACT
GGAAGGGCTCAGAGGGCTCCTCCATAGTCTGCAAGGGCTCCCTGCAGCTGTTCCTGTTCTTCCCTTGGAG
CTGACTGTCACCTGCAACTTCATTATCCTGAGGGCAAGCTTGGCCCAGGGTTTCACAGAGGATCAGGCCC
AGGATATCCAGCGGAGCCTAGAGAGAGTGCTGGAGACACAGGAGCAGCAGGGGCCCAGGTTGGAACAGGG
GCTCAGGGAGCTGTGGGACTCTGTCCTTCGTGCTTCCTGCCTTCTGCCGGAGCTGCTGTCTGCCCTGCAC
CGCCTGGTTGGCCTGCAGGCTGCCCTCTGGTTGAGTGCTGACCGTCTTGGGGACCTGGCCTTGTTACTAG
AGACCCTGAATGGCAGCCAGAGTGGAGCCTCTAAGGATCTGCTGTTACTTCTGAAAACTTGGAGTCCCCC
AGCTGAGGAATTAGATGCTCCATTGACCCTGCAGGATGCCCAGGGATTGAAGGATGTCCTCCTGACAGCA
TTTGCCTACCGCCAAGGTCTCCAGGAGCTGATCACAGGGAACCCAGACAAGGCACTAAGCAGCCTTCATG
AAGCGGCCTCAGGCCTGTGTCCACGGCCTGTGTTGGTCCAGGTGTACACAGCACTGGGGTCCTGTCACCG
TAAGATGGGAAATCCACAGAGAGCACTGTTGTACTTGGTTGCAGCCCTGAAAGAGGGATCAGCCTGGGGT
CCTCCACTTCTGGAGGCCTCTAGGCTCTATCAGCAACTGGGGGACACAACAGCAGAGCTGGAGAGTCTGG
AGCTGCTAGTTGAGGCCTTGAATGTCCCATGCAGTTCCAAAGCCCCGCAGTTTCTCATTGAGGTAGAATT
ACTACTGCCACCACCTGACCTAGCCTCACCCCTTCATTGTGGCACTCAGAGCCAGACCAAGCACATACTA
GCAAGCAGGTGCCTACAGACGGGGAGGGCAGGAGACGCTGCAGAGCATTACTTGGACCTGCTGGCCCTGT
TGCTGGATAGCTCGGAGCCAAGGTTCTCCCCACCCCCCTCCCCTCCAGGGCCCTGTATGCCTGAGGTGTT
TTTGGAGGCAGCGGTAGCACTGATCCAGGCAGGCAGAGCCCAAGATGCCTTGACTCTATGTGAGGAGTTG
CTCAGCCGCACATCATCTCTGCTACCCAAGATGTCCCGGCTGTGGGAAGATGCCAGAAAAGGAACCAAGG
AACTGCCATACTGCCCACTCTGGGTCTCTGCCACCCACCTGCTTCAGGGCCAGGCCTGGGTTCAACTGGG
TGCCCAAAAAGTGGCAATTAGTGAATTTAGCAGGTGCCTCGAGCTGCTCTTCCGGGCCACACCTGAGGAA
AAAGAACAAGGGGCAGCTTTCAACTGTGAGCAGGGATGTAAGTCAGATGCGGCACTGCAGCAGCTTCGGG
CAGCCGCCCTAATTAGTCGTGGACTGGAATGGGTAGCCAGCGGCCAGGATACCAAAGCCTTACAGGACTT
CCTCCTCAGTGTGCAGATGTGCCCAGGTAATCGAGACACTTACTTTCACCTGCTTCAGACTCTGAAGAGG
CTAGATCGGAGGGATGAGGCCACTGCACTCTGGTGGAGGCTGGAGGCCCAAACTAAGGGGTCACATGAAG
ATGCTCTGTGGTCTCTCCCCCTGTACCTAGAAAGCTATTTGAGCTGGATCCGTCCCTCTGATCGTGACGC
CTTCCTTGAAGAATTTCGGACATCTCTGCCAAAGTCTTGTGACCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202443 protein sequence
Red=Cloning site Green=Tags(s)

MSRQTTSVGSSCLDLWREKNDRLVRQAKVAQNSGLTLRRQQLAQDALEGLRGLLHSLQGLPAAVPVLPLE
LTVTCNFIILRASLAQGFTEDQAQDIQRSLERVLETQEQQGPRLEQGLRELWDSVLRASCLLPELLSALH
RLVGLQAALWLSADRLGDLALLLETLNGSQSGASKDLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTA
FAYRQGLQELITGNPDKALSSLHEAASGLCPRPVLVQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWG
PPLLEASRLYQQLGDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKHIL
ASRCLQTGRAGDAAEHYLDLLALLLDSSEPRFSPPPSPPGPCMPEVFLEAAVALIQAGRAQDALTLCEEL
LSRTSSLLPKMSRLWEDARKGTKELPYCPLWVSATHLLQGQAWVQLGAQKVAISEFSRCLELLFRATPEE
KEQGAAFNCEQGCKSDAALQQLRAAALISRGLEWVASGQDTKALQDFLLSVQMCPGNRDTYFHLLQTLKR
LDRRDEATALWWRLEAQTKGSHEDALWSLPLYLESYLSWIRPSDRDAFLEEFRTSLPKSCDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004629
ORF Size 1866 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004629.2
RefSeq Size 2649 bp
RefSeq ORF 1869 bp
Locus ID 2189
UniProt ID O15287
Domains TPR
Protein Families Druggable Genome
MW 68.6 kDa
Gene Summary The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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