SSH3 (NM_017857) Human Tagged ORF Clone
CAT#: RC202399
SSH3 (Myc-DDK-tagged)-Human slingshot homolog 3 (Drosophila) (SSH3)
ORF Plasmid: tGFP
"NM_017857" in other vectors (6)
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CNY 4,904.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SSH3L |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202399 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGGTCACAGTGAGCCGTTCGCCCCCGGGCAGCGGCGCCTCCACGCCCGTGGGGCCCTGGGACC AGGCGGTCCAGCGAAGGAGTCGACTCCAGCGAAGGCAGAGCTTTGCGGTGCTCCGTGGGGCTGTCCTGGG ACTGCAGGATGGAGGGGACAATGATGATGCAGCAGAGGCCAGTTCTGAGCCAACAGAGAAGGCCCCGAGT GAGGAGGAGCTCCACGGGGACCAGACAGACTTCGGGCAAGGATCCCAGAGTCCCCAGAAGCAGGAGGAGC AGAGGCAGCACCTGCACCTCATGGTACAGCTGCTGAGGCCGCAGGATGACATCCGCCTGGCAGCCCAGCT GGAGGCACCCCGGCCTCCCCGGCTCCGCTACCTGCTGGTAGTTTCTACACGAGAAGGAGAAGGTCTGAGC CAGGATGAGACGGTCCTCCTGGGCGTGGATTTCCCTGACAGCAGCTCCCCCAGCTGCACCCTGGGCCTGG TCTTGCCCCTCTGGAGTGACACCCAGGTGTACTTAGATGGAGACGGGGGCTTCAGCGTGACGTCTGGTGG GCAAAGCCGGATCTTCAAGCCCATCTCCATCCAGACCATGTGGGCCACACTCCAGGTATTGCACCAAGCA TGTGAGGCAGCTCTAGGCAGCGGCCTTGTACCGGGTGGCAGTGCCCTCACCTGGGCCAGCCACTACCAGG AGAGACTGAACTCCGAACAGAGCTGCCTCAATGAGTGGACGGCTATGGCCGACCTGGAGTCTCTGCGGCC TCCCAGCGCCGAGCCTGGCGGGTCCTCAGAACAGGAGCAGATGGAGCAGGCGATCCGTGCTGAGCTGTGG AAAGTGTTGGATGTCAGTGACCTGGAGAGTGTCACTTCCAAAGAGATCCGCCAGGCTCTGGAGCTGCGCC TGGGGCTCCCCCTCCAGCAGTACCGTGACTTCATCGACAACCAGATGCTGCTGCTGGTGGCACAGCGGGA CCGAGCCTCCCGCATCTTCCCCCACCTCTACCTGGGCTCAGAGTGGAACGCAGCAAACCTGGAGGAGCTG CAGAGGAACAGGGTCACCCACATCTTGAACATGGCCCGGGAGATTGACAACTTCTACCCTGAGCGCTTCA CCTACCACAATGTGCGCCTCTGGGATGAGGAGTCGGCCCAGCTGCTGCCGCACTGGAAGGAGACGCACCG CTTCATTGAGGCTGCAAGAGCACAGGGCACCCACGTGCTGGTCCACTGCAAGATGGGCGTCAGCCGCTCA GCGGCCACAGTGCTGGCCTATGCCATGAAGCAGTACGAATGCAGCCTGGAGCAGGCCCTGCGCCACGTGC AGGAGCTCCGGCCCATCGCCCGCCCCAACCCTGGCTTCCTGCGCCAGCTGCAGATCTACCAGGGCATCCT GACGGCCAGCCGCCAGAGCCATGTCTGGGAGCAGAAAGTGGGTGGGGTCTCCCCAGAGGAGCACCCAGCC CCTGAAGTCTCTACACCATTCCCACCTCTTCCGCCAGAACCTGAGGGTGGTGGGGAGGAGAAGGTTGTAG GCATGGAAGAGAGCCAGGCAGCCCCGAAAGAAGAGCCTGGGCCACGGCCACGTATAAACCTCCGAGGGGT CATGAGGTCCATCAGTCTTCTGGAGCCCTCCTTGGAGCTGGAGAGCACCTCAGAGACCAGTGACATGCCA GAGGTCTTCTCTTCCCACGAGTCTTCACATGAAGAGCCTCTGCAGCCCTTCCCACAGCTTGCAAGGACCA AGGGAGGCCAGCAGGTGGACAGGGGGCCTCAGCCTGCCCTGAAGTCCCGCCAGTCAGTGGTTACCCTCCA GGGCAGTGCCGTGGTGGCCAACCGGACCCAGGCCTTCCAGGAGCAGGAGCAGGGGCAGGGGCAGGGGCAG GGAGAGCCCTGCATTTCCTCTACGCCCAGGTTCCGGAAGGTGGTGAGACAGGCCAGCGTGCATGACAGTG GAGAGGAGGGCGAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202399 protein sequence
Red=Cloning site Green=Tags(s) MALVTVSRSPPGSGASTPVGPWDQAVQRRSRLQRRQSFAVLRGAVLGLQDGGDNDDAAEASSEPTEKAPS EEELHGDQTDFGQGSQSPQKQEEQRQHLHLMVQLLRPQDDIRLAAQLEAPRPPRLRYLLVVSTREGEGLS QDETVLLGVDFPDSSSPSCTLGLVLPLWSDTQVYLDGDGGFSVTSGGQSRIFKPISIQTMWATLQVLHQA CEAALGSGLVPGGSALTWASHYQERLNSEQSCLNEWTAMADLESLRPPSAEPGGSSEQEQMEQAIRAELW KVLDVSDLESVTSKEIRQALELRLGLPLQQYRDFIDNQMLLLVAQRDRASRIFPHLYLGSEWNAANLEEL QRNRVTHILNMAREIDNFYPERFTYHNVRLWDEESAQLLPHWKETHRFIEAARAQGTHVLVHCKMGVSRS AATVLAYAMKQYECSLEQALRHVQELRPIARPNPGFLRQLQIYQGILTASRQSHVWEQKVGGVSPEEHPA PEVSTPFPPLPPEPEGGGEEKVVGMEESQAAPKEEPGPRPRINLRGVMRSISLLEPSLELESTSETSDMP EVFSSHESSHEEPLQPFPQLARTKGGQQVDRGPQPALKSRQSVVTLQGSAVVANRTQAFQEQEQGQGQGQ GEPCISSTPRFRKVVRQASVHDSGEEGEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017857 |
ORF Size | 1977 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017857.4 |
RefSeq Size | 2894 bp |
RefSeq ORF | 1980 bp |
Locus ID | 54961 |
UniProt ID | Q8TE77 |
Domains | DSPc |
Protein Families | Druggable Genome, Phosphatase |
Protein Pathways | Regulation of actin cytoskeleton |
MW | 73 kDa |
Gene Summary | The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202399L1 | Lenti ORF clone of Human slingshot homolog 3 (Drosophila) (SSH3), Myc-DDK-tagged |
CNY 7,304.00 |
|
RC202399L2 | Lenti ORF clone of Human slingshot homolog 3 (Drosophila) (SSH3), mGFP tagged |
CNY 6,460.00 |
|
RC202399L3 | Lenti ORF clone of Human slingshot homolog 3 (Drosophila) (SSH3), Myc-DDK-tagged |
CNY 6,460.00 |
|
RC202399L4 | Lenti ORF clone of Human slingshot homolog 3 (Drosophila) (SSH3), mGFP tagged |
CNY 6,460.00 |
|
RG202399 | SSH3 (tGFP-tagged) - Human slingshot homolog 3 (Drosophila) (SSH3) |
CNY 5,040.00 |
|
SC111312 | SSH3 (untagged)-Human slingshot homolog 3 (Drosophila) (SSH3) |
CNY 7,220.00 |