SLUG (SNAI2) (NM_003068) Human Tagged ORF Clone

Name: SLUG (SNAI2) (NM_003068) Human Tagged ORF Clone
SKU: RC202365
Price: 2400.00

CAT＃: RC202365

SNAI2 (Myc-DDK-tagged)-Human snail homolog 2 (Drosophila) (SNAI2)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_003068" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00

货期*

现货

规格

Cited in 6 publications.

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

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CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA800167

SNAI2 (SLUG) mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SLUG; SLUGH; SLUGH1; SNAIL2; WS2D
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC202365 representing NM_003068 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCGCTCCTTCCTGGTCAAGAAGCATTTCAACGCCTCCAAAAAGCCAAACTACAGCGAACTGGACA CACATACAGTGATTATTTCCCCGTATCTCTATGAGAGTTACTCCATGCCTGTCATACCACAACCAGAGAT CCTCAGCTCAGGAGCATACAGCCCCATCACTGTGTGGACTACCGCTGCTCCATTCCACGCCCAGCTACCC AATGGCCTCTCTCCTCTTTCCGGATACTCCTCATCTTTGGGGCGAGTGAGTCCCCCTCCTCCATCTGACA CCTCCTCCAAGGACCACAGTGGCTCAGAAAGCCCCATTAGTGATGAAGAGGAAAGACTACAGTCCAAGCT TTCAGACCCCCATGCCATTGAAGCTGAAAAGTTTCAGTGCAATTTATGCAATAAGACCTATTCAACTTTT TCTGGGCTGGCCAAACATAAGCAGCTGCACTGCGATGCCCAGTCTAGAAAATCTTTCAGCTGTAAATACT GTGACAAGGAATATGTGAGCCTGGGCGCCCTGAAGATGCATATTCGGACCCACACATTACCTTGTGTTTG CAAGATCTGCGGCAAGGCGTTTTCCAGACCCTGGTTGCTTCAAGGACACATTAGAACTCACACGGGGGAG AAGCCTTTTTCTTGCCCTCACTGCAACAGAGCATTTGCAGACAGGTCAAATCTGAGGGCTCATCTGCAGA CCCATTCTGATGTAAAGAAATACCAGTGCAAAAACTGCTCCAAAACCTTCTCCAGAATGTCTCTCCTGCA CAAACATGAGGAATCTGGCTGCTGTGTAGCACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC202365 representing NM_003068 Red=Cloning site Green=Tags(s) MPRSFLVKKHFNASKKPNYSELDTHTVIISPYLYESYSMPVIPQPEILSSGAYSPITVWTTAAPFHAQLP NGLSPLSGYSSSLGRVSPPPPSDTSSKDHSGSESPISDEEERLQSKLSDPHAIEAEKFQCNLCNKTYSTF SGLAKHKQLHCDAQSRKSFSCKYCDKEYVSLGALKMHIRTHTLPCVCKICGKAFSRPWLLQGHIRTHTGE KPFSCPHCNRAFADRSNLRAHLQTHSDVKKYQCKNCSKTFSRMSLLHKHEESGCCVAH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_003068
ORF Size	804 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_003068.5
RefSeq Size	2101 bp
RefSeq ORF	807 bp
Locus ID	6591
UniProt ID	O43623
Domains	zf-C2H2
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Adherens junction
MW	29.8 kDa
Gene Summary	This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (6)

The use of this cDNA Clones has been cited in the following citations:
PARP1-SNAI2 transcription axis drives resistance to PARP inhibitor, Talazoparib ,null, Scientific Reports ,PubMed ID 35864202 [SLUG]
YAP promotes epithelial mesenchymal transition by upregulating Slug expression in human colorectal cancer cells ,Cheng, D;Jin, L;Chen, Y;Xi, X;Guo, Y;, Int J Clin Exp Pathol ,PubMed ID 32355518 [SLUG]
FSCN1 is upregulated by SNAI2 and promotes epithelial to mesenchymal transition in head and necksquamous cell carcinoma ,Wang, L;Jia, Y;Jiang, Z;Gao, W;Wang, B;, Cell Biol. Int. ,PubMed ID 28488774 [SLUG]
MicroRNA-124 suppresses Slug-mediated lung cancer metastasis ,Cui, Z;Hu, Y;, Eur Rev Med Pharmacol Sci ,PubMed ID 27735038 [Slug]
Slug contributes to cancer progression by direct regulation of ERα signaling pathway ,null, International Journal of Oncology ,PubMed ID 25652255 [SLUG]
Nuclear factor I-C (NFIC) Regulates Dentin Sialophosphoprotein (DSPP) and E-cadherin via Control of Kruppel-like factor 4 (KLF4) during Dentinogenesis ,Lee, HK;Lee, DS;Park, SJ;Cho, KH;Bae, HS;Park, JC;, J. Biol. Chem. ,PubMed ID 25138274 [SLUG]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC202365L1	Lenti ORF clone of Human snail homolog 2 (Drosophila) (SNAI2), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RC202365L2	Lenti ORF clone of Human snail homolog 2 (Drosophila) (SNAI2), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RC202365L3	Lenti ORF clone of Human snail homolog 2 (Drosophila) (SNAI2), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RC202365L4	Lenti ORF clone of Human snail homolog 2 (Drosophila) (SNAI2), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RG202365	SNAI2 (tGFP-tagged) - Human snail homolog 2 (Drosophila) (SNAI2)	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,000.00 CNY 4,370.00
SC118205	SNAI2 (untagged)-Human snail homolog 2 (Drosophila) (SNAI2)	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,600.00