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GAPDH (NM_002046) Human Tagged ORF Clone
CAT＃: RC202309
GAPDH (Myc-DDK-tagged)-Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
ORF Plasmid: tGFP
"NM_002046" in other vectors (6)
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CNY 5,488.00
| Cited in 7 publications. |
Product images
CNY 300.00
CNY 600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | G3PD; GAPD; HEL-S-162eP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202309 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTCACCAGGGCTGCTTTTA ACTCTGGTAAAGTGGATATTGTTGCCATCAATGACCCCTTCATTGACCTCAACTACATGGTTTACATGTT CCAATATGATTCCACCCATGGCAAATTCCATGGCACCGTCAAGGCTGAGAACGGGAAGCTTGTCATCAAT GGAAATCCCATCACCATCTTCCAGGAGCGAGATCCCTCCAAAATCAAGTGGGGCGATGCTGGCGCTGAGT ACGTCGTGGAGTCCACTGGCGTCTTCACCACCATGGAGAAGGCTGGGGCTCATTTGCAGGGGGGAGCCAA AAGGGTCATCATCTCTGCCCCCTCTGCTGATGCCCCCATGTTCGTCATGGGTGTGAACCATGAGAAGTAT GACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCACCACCAACTGCTTAGCACCCCTGGCCAAGGTCA TCCATGACAACTTTGGTATCGTGGAAGGACTCATGACCACAGTCCATGCCATCACTGCCACCCAGAAGAC TGTGGATGGCCCCTCCGGGAAACTGTGGCGTGATGGCCGCGGGGCTCTCCAGAACATCATCCCTGCCTCT ACTGGCGCTGCCAAGGCTGTGGGCAAGGTCATCCCTGAGCTGAACGGGAAGCTCACTGGCATGGCCTTCC GTGTCCCCACTGCCAACGTGTCAGTGGTGGACCTGACCTGCCGTCTAGAAAAACCTGCCAAATATGATGA CATCAAGAAGGTGGTGAAGCAGGCGTCGGAGGGCCCCCTCAAGGGCATCCTGGGCTACACTGAGCACCAG GTGGTCTCCTCTGACTTCAACAGCGACACCCACTCCTCCACCTTTGACGCTGGGGCTGGCATTGCCCTCA ACGACCACTTTGTCAAGCTCATTTCCTGGTATGACAACGAATTTGGCTACAGCAACAGGGTGGTGGACCT CATGGCCCACATGGCCTCCAAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202309 protein sequence
Red=Cloning site Green=Tags(s) MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVIN GNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKY DNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPAS TGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQ VVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002046 |
| ORF Size | 1005 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002046.7 |
| RefSeq Size | 1421 bp |
| RefSeq ORF | 1008 bp |
| Locus ID | 2597 |
| UniProt ID | P04406 |
| Domains | gpdh |
| Protein Families | ES Cell Differentiation/IPS |
| Protein Pathways | Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways |
| MW | 36.1 kDa |
| Gene Summary | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
Citations (7)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Antitumor Activity of Asperphenin B by Induction of Apoptosis and Regulation of Glyceraldehyde-3-phosphate Dehydrogenase in Human Colorectal Cancer Cells
,null,
Journal of Natural Products
,PubMed ID 33398999
[GAPDH]
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GAPDH controls extracellular vesicle biogenesis and enhances therapeutic potential of EVs in silencing the Huntingtin gene in mice via siRNA delivery
,Dar, GH;Mendes, CC;Kuan, WL;Conceicao, M;,
bioRxiv
[GAPDH]
|
|
Malonylation of GAPDH is an inflammatory signal in macrophages
,Galván-Peña, S;Carroll, RG;Newman, C;Hinchy, EC;Palsson-McDermott, E;Robinson, EK;Covarrubias, S;Nadin, A;James, AM;Haneklaus, M;Carpenter, S;Kelly, VP;Murphy, MP;Modis, LK;O'Neill, LA;,
Nat Commun
,PubMed ID 30659183
[GAPDH]
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GAPDH with NAD+-binding site mutation competitively inhibits the wild-type and affects glucose metabolism in cancer
,Kunjithapatham, R;Ganapathy-Kanniappan, S;,
Biochim. Biophys. Acta
,PubMed ID 30077773
[GAPDH]
|
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Protein CoAlation: A Redox-Regulated Protein Modification by Coenzyme Ain Mammalian Cells
,Tsuchiya, Y;Peak-Chew, S;Newell, C;Miller-Aidoo, S;Mangal, S;Zhyvoloup, A;Baković, J;Malanchuk, O;Pereira, G;Kotiadis, V;Szabadkai, G;Duchen, M;Campbell, M;Rodriguez Cuenca, S;Vidal-Puig, A;James, A;Murphy, MP;Filonenko, V;Skehel, M;Gout, I;,
Biochem. J
,PubMed ID 28341808
[GAPDH]
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Overexpression of glyceraldehyde 3-phosphate dehydrogenase prevents neurovascular degeneration after retinal injury
,Cai, R;Xue, W;Liu, S;Petersen, RB;Huang, K;Zheng, L;,
FASEB J.
,PubMed ID 25805836
[GAPDH]
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Glyceraldehyde-3-phosphate dehydrogenase acts as a mitochondrial trans-s-nitrosylase in the heart
,Kohr, MJ;Murphy, E;Steenbergen, C;,
PLoS ONE
,PubMed ID 25347796
[GAPDH]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202309L1 | Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC202309L2 | Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mGFP tagged |
CNY 7,888.00 |
|
| RC202309L3 | Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC202309L4 | Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mGFP tagged |
CNY 7,888.00 |
|
| RG202309 | GAPDH (tGFP-tagged) - Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
CNY 7,088.00 |
|
| SC118869 | GAPDH (untagged)-Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
CNY 3,656.00 |
