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ARHGEF19 (NM_153213) Human Tagged ORF Clone
CAT＃: RC202273
ARHGEF19 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 19 (ARHGEF19)
ORF Plasmid: tGFP
"NM_153213" in other vectors (6)
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CNY 5,872.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | WGEF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202273 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTGTGGGCCACCTGCTACCCTCCAGCCCCACCTGACTGGGCCACCTGGCACTGCCCACCACCCTG TAGCAGTGTGCCAGCAGGAGAGTCTGTCCTTTGCAGAGCTGCCCGCCCTGAAGCCCCCGAGCCCAGTGTG TCTGGACCTTTTCCCTGTTGCCCCAGAGGAGCTTCGGGCTCCTGGCAGCCGCTGGTCCCTGGGGACCCCT GCCCCTCTCCAAGGGTTGCTATGGCCATTATCCCCAGGAGGCTCAGATACAGAGATCACCAGCGGGGGGA TGCGGCCCAGCAGGGCTGGCAGCTGGCCACACTGTCCTGGTGCCCAGCCCCCAGCTCTGGAGGGACCCTG GAGTCCCCGACACACACAGCCACAGCGCCGGGCCAGCCACGGCTCGGAGAAGAAGTCTGCCTGGCGCAAG ATGCGGGTGTACCAGCGTGAAGAGGTCCCCGGCTGCCCCGAGGCCCACGCTGTCTTCCTAGAGCCTGGCC AGGTAGTGCAAGAGCAGGCCCTGAGCACAGAGGAGCCCAGGGTGGAGTTGTCTGGGTCCACCCGAGTGAG CCTCGAAGGTCCTGAGCGGAGGCGCTTCTCGGCATCGGAGCTGATGACCCGGCTGCACTCTTCTCTGCGC CTGGGGCGGAATTCAGCAGCCCGGGCACTCATCTCTGGGTCAGGCACCGGAGCAGCCCGGGAAGGGAAAG CATCTGGAATGGAGGCTCGAAGTGTAGAGATGAGCGGGGACCGGGTGTCGCGGCCAGCCCCTGGTGACTC ACGAGAGGGCGATTGGTCCGAGCCCAGGCTAGACACACAGGAAGAGCCGCCTTTGGGGTCCAGGAGCACC AACGAGCGGCGCCAGTCTCGATTCCTCCTTAACTCCGTCCTCTATCAGGAATACAGCGACGTGGCCAGCG CCCGCGAACTGCGGCGGCAGCAGCGCGAGGAGGAGGGCCCGGGGGACGAGGCCGAGGGCGCAGAGGAGGG GCCGGGGCCGCCGCGGGCCAACCTCTCCCCCAGCAGCTCCTTCCGGGCGCAGCGCTCGGCGCGAGGCTCC ACCTTCTCGCTGTGGCAGGATATCCCCGACGTACGCGGCAGCGGCGTCCTGGCCACGCTGAGCCTGCGGG ACTGCAAGCTGCAGGAGGCCAAGTTTGAGCTGATCACCTCCGAGGCCTCCTACATCCACAGCCTGTCGGT GGCTGTGGGCCACTTCTTAGGCTCTGCCGAGCTGAGCGAGTGTCTGGGGGCGCAGGACAAGCAGTGGCTG TTTTCCAAACTGCCCGAGGTCAAGAGCACCAGCGAGAGGTTCCTGCAGGACCTGGAGCAGCGGCTGGAGG CAGATGTGCTGCGCTTCAGCGTGTGCGACGTGGTGCTGGACCACTGCCCGGCCTTCCGCAGAGTCTACCT GCCCTATGTCACCAACCAGGCCTACCAGGAGCGCACCTACCAGCGCCTGCTCCTGGAGAACCCCAGGTTC CCTGGCATCCTGGCTCGCCTGGAGGAGTCTCCTGTGTGCCAGCGTCTGCCCCTTACCTCCTTCCTTATCC TGCCCTTCCAGAGGATCACCCGCCTCAAGATGTTGGTGGAGAACATCCTGAAGCGGACAGCACAGGGCTC TGAAGACGAAGACATGGCCACCAAGGCCTTCAATGCGCTCAAGGAGCTGGTGCAGGAGTGCAATGCTAGT GTACAGTCCATGAAGAGGACAGAGGAACTCATCCACCTGAGCAAGAAGATCCACTTTGAGGGCAAGATTT TCCCGCTGATCTCTCAGGCCCGCTGGCTGGTTCGGCATGGAGAGTTGGTAGAGCTGGCACCACTGCCTGC AGCACCCCCTGCCAAGCTGAAGCTGTCCAGCAAGGCAGTCTACCTCCACCTCTTCAATGACTGCTTGCTG CTCTCTCGGCGGAAGGAGCTAGGGAAGTTTGCCGTTTTCGTCCATGCCAAGATGGCTGAGCTGCAGGTGC GGGACCTGAGCCTGAAGCTGCAGGGCATCCCCGGCCACGTGTTCCTCCTCCAGCTCCTCCACGGGCAGCA CATGAAGCACCAGTTCCTGCTGCGGGCCCGGACGGAAAGTGAGAAGCAGCGATGGATCTCAGCCTTGTGC CCCTCCAGCCCCCAGGAGGACAAGGAGGTCATCAGTGAGGGGGAAGATTGCCCCCAGGTTCAGTGTGTTA GGACATACAAGGCACTGCACCCAGATGAGCTGACCTTGGAGAAGACTGACATCCTGTCAGTGAGGACCTG GACCAGTGACGGCTGGCTGGAAGGGGTCCGCCTGGCAGATGGTGAGAAGGGGTGGGTGCCCCAGGCCTAT GTGGAAGAGATCAGCAGCCTCAGCGCCCGCCTCCGAAACCTCCGGGAGAATAAGCGAGTCACAAGTGCCA CCAGCAAACTGGGGGAGGCTCCTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202273 protein sequence
Red=Cloning site Green=Tags(s) MDCGPPATLQPHLTGPPGTAHHPVAVCQQESLSFAELPALKPPSPVCLDLFPVAPEELRAPGSRWSLGTP APLQGLLWPLSPGGSDTEITSGGMRPSRAGSWPHCPGAQPPALEGPWSPRHTQPQRRASHGSEKKSAWRK MRVYQREEVPGCPEAHAVFLEPGQVVQEQALSTEEPRVELSGSTRVSLEGPERRRFSASELMTRLHSSLR LGRNSAARALISGSGTGAAREGKASGMEARSVEMSGDRVSRPAPGDSREGDWSEPRLDTQEEPPLGSRST NERRQSRFLLNSVLYQEYSDVASARELRRQQREEEGPGDEAEGAEEGPGPPRANLSPSSSFRAQRSARGS TFSLWQDIPDVRGSGVLATLSLRDCKLQEAKFELITSEASYIHSLSVAVGHFLGSAELSECLGAQDKQWL FSKLPEVKSTSERFLQDLEQRLEADVLRFSVCDVVLDHCPAFRRVYLPYVTNQAYQERTYQRLLLENPRF PGILARLEESPVCQRLPLTSFLILPFQRITRLKMLVENILKRTAQGSEDEDMATKAFNALKELVQECNAS VQSMKRTEELIHLSKKIHFEGKIFPLISQARWLVRHGELVELAPLPAAPPAKLKLSSKAVYLHLFNDCLL LSRRKELGKFAVFVHAKMAELQVRDLSLKLQGIPGHVFLLQLLHGQHMKHQFLLRARTESEKQRWISALC PSSPQEDKEVISEGEDCPQVQCVRTYKALHPDELTLEKTDILSVRTWTSDGWLEGVRLADGEKGWVPQAY VEEISSLSARLRNLRENKRVTSATSKLGEAPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153213 |
| ORF Size | 2406 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153213.5 |
| RefSeq Size | 3044 bp |
| RefSeq ORF | 2409 bp |
| Locus ID | 128272 |
| UniProt ID | Q8IW93 |
| MW | 89.2 kDa |
| Gene Summary | Guanine nucleotide exchange factors (GEFs) such as ARHGEF19 accelerate the GTPase activity of Rho GTPases (see RHOA, MIM 165390).[supplied by OMIM, Dec 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
miR-503 regulates metastatic function through Rho guanine nucleotide exchanger factor 19 in hepatocellular carcinoma.
,null,
The Journal of surgical research
,PubMed ID 24405610
[ARHGEF19]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202273L1 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 19 (ARHGEF19), Myc-DDK-tagged |
CNY 8,272.00 |
|
| RC202273L2 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 19 (ARHGEF19), mGFP tagged |
CNY 7,700.00 |
|
| RC202273L3 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 19 (ARHGEF19), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC202273L4 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 19 (ARHGEF19), mGFP tagged |
CNY 7,700.00 |
|
| RG202273 | ARHGEF19 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 19 (ARHGEF19) |
CNY 6,370.00 |
|
| SC306560 | ARHGEF19 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 19 (ARHGEF19) |
CNY 7,220.00 |
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