FBXO8 (NM_012180) Human Tagged ORF Clone
CAT#: RC202154
FBXO8 (Myc-DDK-tagged)-Human F-box protein 8 (FBXO8)
ORF Plasmid: tGFP
"NM_012180" in other vectors (7)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DC10; FBS; FBX8 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202154 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCAAGGGTTGTGGAGAGTGGTCAGAAACCAGCAGCTGCAACAAGAAGGCTACAGTGAGCAAGGCT ACCTCACCAGAGAGCAGAGCAGGAGAATGGCTGCGAGCAACATTTCTAACACCAATCATCGTAAACAAGT CCAAGGAGGCATTGACATATATCATCTTTTGAAGGCAAGGAAATCGAAAGAACAGGAAGGATTCATTAAT TTGGAAATGTTGCCTCCTGAGCTAAGCTTTACCATCTTGTCCTACCTGAATGCAACTGACCTTTGCTTGG CTTCATGTGTTTGGCAGGACCTTGCGAATGATGAACTTCTCTGGCAAGGGTTGTGCAAATCCACTTGGGG TCACTGTTCCATATACAATAAGAACCCACCTTTAGGATTTTCTTTTAGAAAATTGTATATGCAGCTGGAT GAAGGCAGCCTCACCTTTAATGCCAACCCAGATGAGGGAGTGAACTACTTTATGTCCAAGGGTATCCTGG ATGATTCGCCAAAGGAAATAGCAAAGTTTATCTTCTGTACAAGAACACTAAATTGGAAAAAACTGAGAAT CTATCTTGATGAAAGGAGAGATGTCTTGGATGACCTTGTAACATTGCATAATTTTAGAAATCAGTTCTTG CCAAATGCACTGAGAGAATTTTTTCGTCATATCCATGCCCCTGAAGAGCGTGGAGAGTATCTTGAAACTC TTATAACAAAGTTCTCACATAGATTCTGTGCTTGCAACCCTGATTTAATGCGAGAACTTGGCCTTAGTCC TGATGCTGTCTATGTACTGTGCTACTCTTTGATTCTACTTTCCATTGACCTCACTAGCCCTCATGTGAAG AATAAAATGTCAAAAAGGGAATTTATTCGAAATACCCGTCGCGCTGCTCAAAATATTAGTGAAGATTTTG TAGGGCATCTTTATGACAATATCTACCTTATTGGCCATGTGGCTGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202154 protein sequence
Red=Cloning site Green=Tags(s) MGQGLWRVVRNQQLQQEGYSEQGYLTREQSRRMAASNISNTNHRKQVQGGIDIYHLLKARKSKEQEGFIN LEMLPPELSFTILSYLNATDLCLASCVWQDLANDELLWQGLCKSTWGHCSIYNKNPPLGFSFRKLYMQLD EGSLTFNANPDEGVNYFMSKGILDDSPKEIAKFIFCTRTLNWKKLRIYLDERRDVLDDLVTLHNFRNQFL PNALREFFRHIHAPEERGEYLETLITKFSHRFCACNPDLMRELGLSPDAVYVLCYSLILLSIDLTSPHVK NKMSKREFIRNTRRAAQNISEDFVGHLYDNIYLIGHVAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012180 |
ORF Size | 957 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012180.3 |
RefSeq Size | 2564 bp |
RefSeq ORF | 960 bp |
Locus ID | 26269 |
UniProt ID | Q9NRD0 |
Domains | Sec7, F-box |
Protein Families | Druggable Genome |
MW | 37.1 kDa |
Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It contains a C-terminal amino acid sequence that bears a significant similarity with a portion of yeast Sec7p, a critical regulator of vesicular protein transport. This human protein may interact with ADP-ribosylation factor(s)(ARFs) and exhibit ARF-GEF (guanine nucleotide exchange factor) activity. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202154L1 | Lenti ORF clone of Human F-box protein 8 (FBXO8), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC202154L2 | Lenti ORF clone of Human F-box protein 8 (FBXO8), mGFP tagged |
CNY 5,890.00 |
|
RC202154L3 | Lenti ORF clone of Human F-box protein 8 (FBXO8), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202154L4 | Lenti ORF clone of Human F-box protein 8 (FBXO8), mGFP tagged |
CNY 5,890.00 |
|
RG202154 | FBXO8 (tGFP-tagged) - Human F-box protein 8 (FBXO8) |
CNY 4,000.00 |
|
SC101002 | FBXO8 (untagged)-Human F-box protein 8 (FBXO8) |
CNY 2,400.00 |
|
SC322334 | FBXO8 (untagged)-Human F-box protein 8 (FBXO8) |
CNY 2,950.00 |