SERPINB1 (NM_030666) Human Tagged ORF Clone

CAT#: RC202138

SERPINB1 (Myc-DDK-tagged)-Human serpin peptidase inhibitor, clade B (ovalbumin), member 1 (SERPINB1)

ORF Plasmid: DDK tGFP



  "NM_030666" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


SERPINB1 mouse monoclonal antibody, clone OTI3B4 (formerly 3B4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms EI; ELANH2; HEL-S-27; HEL57; LEI; M/NEI; MNEI; PI-2; PI2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202138 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCAGCTGAGCTCAGCAAACACCCGCTTCGCCTTGGACCTGTTCCTGGCGTTGAGTGAGAACAATC
CGGCTGGAAACATCTTCATCTCTCCCTTCAGCATTTCATCTGCTATGGCCATGGTTTTTCTGGGGACCAG
AGGTAACACGGCAGCACAGCTGTCCAAGACTTTCCATTTCAACACGGTTGAAGAGGTTCATTCAAGATTC
CAGAGTCTGAATGCTGATATCAACAAACGTGGAGCGTCTTATATTCTGAAACTTGCTAATAGATTATATG
GAGAGAAAACTTACAATTTCCTTCCTGAGTTCTTGGTTTCGACTCAGAAAACATATGGTGCTGACCTGGC
CAGTGTGGATTTTCAGCATGCCTCTGAAGATGCAAGGAAGACCATAAACCAGTGGGTCAAAGGACAGACA
GAAGGAAAAATTCCGGAACTGTTGGCTTCGGGCATGGTTGATAACATGACCAAACTTGTGCTAGTAAATG
CCATCTATTTCAAGGGAAACTGGAAGGATAAATTCATGAAAGAAGCCACGACGAATGCACCATTCAGATT
GAATAAGAAAGACAGAAAAACTGTGAAAATGATGTATCAGAAGAAAAAATTTGCATATGGCTACATCGAG
GACCTTAAGTGCCGTGTGCTGGAACTGCCTTACCAAGGCGAGGAGCTCAGCATGGTCATCCTGCTGCCGG
ATGACATTGAGGACGAGTCCACGGGCCTGAAGAAGATTGAGGAACAGTTGACTTTGGAAAAGTTGCATGA
GTGGACTAAACCTGAGAATCTCGATTTCATTGAAGTTAATGTCAGCTTGCCCAGGTTCAAACTGGAAGAG
AGTTACACTCTCAACTCCGACCTCGCCCGCCTAGGTGTGCAGGATCTCTTTAACAGTAGCAAGGCTGATC
TGTCTGGCATGTCAGGAGCCAGAGATATTTTTATATCAAAAATTGTCCACAAGTCATTTGTGGAAGTGAA
TGAAGAGGGAACAGAGGCGGCAGCTGCCACAGCAGGCATCGCAACTTTCTGCATGTTGATGCCCGAAGAA
AATTTCACTGCCGACCATCCATTCCTTTTCTTTATTCGGCATAATTCCTCAGGTAGCATCCTATTCTTGG
GGAGATTTTCTTCCCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202138 protein sequence
Red=Cloning site Green=Tags(s)

MEQLSSANTRFALDLFLALSENNPAGNIFISPFSISSAMAMVFLGTRGNTAAQLSKTFHFNTVEEVHSRF
QSLNADINKRGASYILKLANRLYGEKTYNFLPEFLVSTQKTYGADLASVDFQHASEDARKTINQWVKGQT
EGKIPELLASGMVDNMTKLVLVNAIYFKGNWKDKFMKEATTNAPFRLNKKDRKTVKMMYQKKKFAYGYIE
DLKCRVLELPYQGEELSMVILLPDDIEDESTGLKKIEEQLTLEKLHEWTKPENLDFIEVNVSLPRFKLEE
SYTLNSDLARLGVQDLFNSSKADLSGMSGARDIFISKIVHKSFVEVNEEGTEAAAATAGIATFCMLMPEE
NFTADHPFLFFIRHNSSGSILFLGRFSSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_030666
ORF Size 1137 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_030666.4
RefSeq Size 2678 bp
RefSeq ORF 1140 bp
Locus ID 1992
UniProt ID P30740
Domains SERPIN
Protein Families Druggable Genome
MW 42.7 kDa
Gene Summary The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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