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SPINT2 (NM_021102) Human Tagged ORF Clone
CAT＃: RC202044
SPINT2 (Myc-DDK-tagged)-Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a
ORF Plasmid: tGFP
"NM_021102" in other vectors (7)
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CNY 2,400.00
CNY 3,705.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DIAR3; HAI-2; HAI2; Kop; PB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202044 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGCTGTGCGGGCTGAGGCGGAGCCGGGCGTTTCTCGCCCTGCTGGGATCGCTGCTCCTCTCTG GGGTCCTGGCGGCCGACCGAGAACGCAGCATCCACGACTTCTGCCTGGTGTCGAAGGTGGTGGGCAGATG CCGGGCCTCCATGCCTAGGTGGTGGTACAATGTCACTGACGGATCCTGCCAGCTGTTTGTGTATGGGGGC TGTGACGGAAACAGCAATAATTACCTGACCAAGGAGGAGTGCCTCAAGAAATGTGCCACTGTCACAGAGA ATGCCACGGGTGACCTGGCCACCAGCAGGAATGCAGCGGATTCCTCTGTCCCAAGTGCTCCCAGAAGGCA GGATTCTGAAGACCACTCCAGCGATATGTTCAACTATGAAGAATACTGCACCGCCAACGCAGTCACTGGG CCTTGCCGTGCATCCTTCCCACGCTGGTACTTTGACGTGGAGAGGAACTCCTGCAATAACTTCATCTATG GAGGCTGCCGGGGCAATAAGAACAGCTACCGCTCTGAGGAGGCCTGCATGCTCCGCTGCTTCCGCCAGCA GGAGAATCCTCCCCTGCCCCTTGGCTCAAAGGTGGTGCTTCTGGCGGGGCTGTTCGTGATGGTGTTGATC CTCTTCCTGGGAGCCTCCATGGTCTACCTGATCCGGGTGGCACGGAGGAACCAGGAGCGTGCCCTGCGCA CCGTCTGGAGCTCCGGAGATGACAAGGAGCAGCTGGTGAAGAACACATATGTCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202044 protein sequence
Red=Cloning site Green=Tags(s) MAQLCGLRRSRAFLALLGSLLLSGVLAADRERSIHDFCLVSKVVGRCRASMPRWWYNVTDGSCQLFVYGG CDGNSNNYLTKEECLKKCATVTENATGDLATSRNAADSSVPSAPRRQDSEDHSSDMFNYEEYCTANAVTG PCRASFPRWYFDVERNSCNNFIYGGCRGNKNSYRSEEACMLRCFRQQENPPLPLGSKVVLLAGLFVMVLI LFLGASMVYLIRVARRNQERALRTVWSSGDDKEQLVKNTYVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021102 |
| ORF Size | 756 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021102.4 |
| RefSeq Size | 1817 bp |
| RefSeq ORF | 759 bp |
| Locus ID | 10653 |
| UniProt ID | O43291 |
| Domains | KU |
| Protein Families | Transmembrane |
| MW | 28.2 kDa |
| Gene Summary | This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Targeted HAI-2 deletion causes excessive proteolysis with prolonged active prostasin and depletion of HAI-1 monomer in intestinal but not epidermal epithelial cells
,Barndt, RB;Lee, MJ;Huang, N;Lu, DD;Lee, SC;Du, PW;Chang, CC;Tsai, PB;Huang, YK;Chang, HM;Wang, JK;Lai, CH;Johnson, MD;Lin, CY;,
Human molecular genetics
,PubMed ID 34089062
[SPINT2]
|
|
Matriptase-mediated cleavage of EpCAM destabilizes claudins and dysregulates intestinal epithelial homeostasis
,Wu, CJ;Feng, X;Lu, M;Morimura, S;Udey, MC;,
J. Clin. Invest
,PubMed ID 28094766
[SPINT2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202044L1 | Lenti ORF clone of Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC202044L2 | Lenti ORF clone of Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a, mGFP tagged |
CNY 5,890.00 |
|
| RC202044L3 | Lenti ORF clone of Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202044L4 | Lenti ORF clone of Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a, mGFP tagged |
CNY 4,800.00 |
|
| RG202044 | SPINT2 (tGFP-tagged) - Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a |
CNY 4,000.00 |
|
| SC112961 | SPINT2 (untagged)-Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a |
CNY 2,400.00 |
|
| SC322322 | SPINT2 (untagged)-Human serine peptidase inhibitor, Kunitz type, 2 (SPINT2), transcript variant a |
CNY 2,400.00 |
