DREF (ZBED1) (NM_004729) Human Tagged ORF Clone
CAT#: RC202011
ZBED1 (Myc-DDK-tagged)-Human zinc finger, BED-type containing 1 (ZBED1), transcript variant 2
ORF Plasmid: tGFP
"NM_004729" in other vectors (4)
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CNY 5,088.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ALTE; DREF; hDREF; TRAMP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202011 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAATAAAAGCCTGGAGAGCTCCCAGACAGACCTGAAGCTGGTGGCCCACCCCCGCGCCAAGAGCA AGGTGTGGAAGTATTTCGGCTTCGACACCAACGCCGAGGGATGCATCCTGCAGTGGAAGAAAATCTACTG CCGCATCTGCATGGCCCAGATCGCCTACTCCGGAAACACCTCCAACCTGTCCTACCACCTGGAGAAGAAC CACCCCGAGGAATTCTGCGAGTTCGTCAAGAGCAACACGGAGCAGATGCGTGAAGCCTTCGCCACCGCCT TCTCCAAGCTGAAGCCCGAGTCGTCCCAGCAGCCCGGGCAGGATGCGCTGGCCGTCAAGGCCGGCCACGG CTACGACAGCAAGAAGCAGCAGGAGCTGACGGCCGCCGTGCTGGGCCTCATCTGCGAGGGGCTGTACCCA GCCTCCATCGTGGACGAGCCCACCTTCAAGGTGCTGCTGAAGACGGCCGACCCCCGGTATGAGCTGCCCA GCCGGAAGTACATCTCTACCAAGGCCATCCCTGAGAAGTACGGGGCCGTCCGGGAGGTGATCCTGAAGGA GCTGGCCGAGGCCACCTGGTGTGGCATCTCCACCGACATGTGGAGGAGTGAGAATCAGAACCGCGCCTAC GTCACGCTGGCCGCCCACTTCCTGGGCCTGGGCGCCCCCAACTGCCTGTCCATGGGCTCCCGCTGCCTGA AGACCTTCGAGGTGCCCGAAGAGAACACGGCGGAGACCATCACGCGAGTGCTCTATGAGGTCTTCATCGA GTGGGGCATCAGCGCCAAGGTCTTCGGGGCCACCACCAACTATGGCAAGGACATCGTGAAGGCGTGCTCC CTGCTGGACGTCGCAGTGCACATGCCCTGCCTGGGCCACACCCTCAATGCCGGCATCCAGCAGGCCTTCC AGCTCCCGAAGCTGGGGGCGCTGCTGTCGCGCTGCCGCAAACTGGTGGAGTACTTCCAGCAGTCTGCCGT GGCCATGTACATGCTCTATGAGAAGCAGAAGCAGCAGAACGTGGCCCACTGCATGCTGGTGAGCAACCGC GTCTCCTGGTGGGGGAGCACGCTGGCCATGCTGCAGCGCCTCAAGGAGCAGCAGTTCGTCATCGCCGGGG TCTTGGTGGAGGACAGCAACAACCACCACCTCATGCTGGAGGCCAGCGAGTGGGCCACCATCGAGGGGCT GGTGGAGCTCCTGCAGCCCTTCAAGCAGGTGGCCGAGATGCTGTCGGCCTCCAGGTACCCCACCATCAGC ATGGTGAAGCCGCTGCTGCACATGCTCCTGAACACCACGCTCAACATCAAGGAGACCGACTCCAAGGAGC TCAGCATGGCCAAGGAGGTCATCGCCAAGGAGCTTTCCAAGACCTACCAGGAGACGCCCGAGATCGACAT GTTTCTCAACGTGGCCACCTTCCTGGACCCCCGCTACAAGAGGCTGCCCTTCCTCTCCGCCTTCGAGCGG CAGCAGGTGGAGAATCGCGTGGTGGAAGAGGCCAAGGGCCTGCTGGACAAGGTCAAAGACGGCGGCTACC GGCCGGCTGAGGACAAGATCTTCCCGGTGCCCGAGGAGCCTCCCGTCAAGAAGCTCATGCGGACATCCAC GCCGCCGCCCGCCAGCGTCATCAACAACATGCTGGCCGAGATCTTCTGCCAGACAGGCGGCGTGGAGGAC CAGGAAGAGTGGCATGCCCAGGTGGTGGAGGAGCTGAGCAACTTCAAGTCCCAGAAGGTGCTTGGCCTCA ACGAAGACCCCCTCAAGTGGTGGTCAGACCGCCTGGCCCTCTTCCCCCTGCTGCCCAAGGTGCTGCAGAA GTACTGGTGCGTGACGGCCACGCGCGTCGCCCCTGAGCGTCTCTTCGGATCCGCCGCCAACGTGGTCAGC GCCAAGAGGAACCGGCTGGCTCCCGCGCACGTGGACGAGCAGGTGTTTCTGTATGAGAACGCCCGGAGTG GGGCAGAGGCGGAACCCGAGGACCAGGACGAGGGGGAGTGGGGCCTGGACCAGGAGCAGGTGTTCTCCTT GGGGGATGGCGTCAGCGGCGGTTTCTTTGGCATTAGGGACAGCAGCTTCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202011 protein sequence
Red=Cloning site Green=Tags(s) MENKSLESSQTDLKLVAHPRAKSKVWKYFGFDTNAEGCILQWKKIYCRICMAQIAYSGNTSNLSYHLEKN HPEEFCEFVKSNTEQMREAFATAFSKLKPESSQQPGQDALAVKAGHGYDSKKQQELTAAVLGLICEGLYP ASIVDEPTFKVLLKTADPRYELPSRKYISTKAIPEKYGAVREVILKELAEATWCGISTDMWRSENQNRAY VTLAAHFLGLGAPNCLSMGSRCLKTFEVPEENTAETITRVLYEVFIEWGISAKVFGATTNYGKDIVKACS LLDVAVHMPCLGHTLNAGIQQAFQLPKLGALLSRCRKLVEYFQQSAVAMYMLYEKQKQQNVAHCMLVSNR VSWWGSTLAMLQRLKEQQFVIAGVLVEDSNNHHLMLEASEWATIEGLVELLQPFKQVAEMLSASRYPTIS MVKPLLHMLLNTTLNIKETDSKELSMAKEVIAKELSKTYQETPEIDMFLNVATFLDPRYKRLPFLSAFER QQVENRVVEEAKGLLDKVKDGGYRPAEDKIFPVPEEPPVKKLMRTSTPPPASVINNMLAEIFCQTGGVED QEEWHAQVVEELSNFKSQKVLGLNEDPLKWWSDRLALFPLLPKVLQKYWCVTATRVAPERLFGSAANVVS AKRNRLAPAHVDEQVFLYENARSGAEAEPEDQDEGEWGLDQEQVFSLGDGVSGGFFGIRDSSFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_004729 |
ORF Size | 2082 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_004729.4 |
RefSeq Size | 4525 bp |
RefSeq ORF | 2085 bp |
Locus ID | 9189 |
UniProt ID | O96006 |
Domains | zf-BED |
Protein Families | Druggable Genome |
MW | 78.1 kDa |
Gene Summary | This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene. [provided by RefSeq, Jan 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202011L3 | Lenti ORF clone of Human zinc finger, BED-type containing 1 (ZBED1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202011L4 | Lenti ORF clone of Human zinc finger, BED-type containing 1 (ZBED1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG202011 | ZBED1 (tGFP-tagged) - Human zinc finger, BED-type containing 1 (ZBED1), transcript variant 2 |
CNY 4,370.00 |
|
SC117180 | ZBED1 (untagged)-Human zinc finger, BED-type containing 1 (ZBED1), transcript variant 2 |
CNY 5,088.00 |