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ABHD5 (NM_016006) Human Tagged ORF Clone
CAT＃: RC201869
ABHD5 (Myc-DDK-tagged)-Human abhydrolase domain containing 5 (ABHD5)
ORF Plasmid: tGFP
"NM_016006" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGI58; IECN2; NCIE2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201869 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAGGAGGAGGAGGTGGACTCTGCCGACACCGGAGAGAGGTCAGGATGGCTAACTGGTTGGC TCCCCACATGGTGCCCTACGTCTATATCACACCTTAAAGAAGCTGAAGAGAAGATGTTAAAATGTGTGCC TTGCACATACAAAAAAGAACCTGTTCGTATATCTAATGGAAATAAAATATGGACACTGAAGTTCTCTCAT AATATTTCAAATAAGACTCCACTTGTCCTTCTCCATGGTTTTGGAGGAGGTCTTGGGCTCTGGGCACTGA ATTTTGGAGATCTTTGCACCAACAGACCTGTCTATGCTTTTGACCTATTGGGTTTTGGACGAAGTAGTAG ACCCAGGTTTGACAGTGATGCAGAAGAAGTGGAGAATCAGTTTGTGGAATCCATTGAAGAGTGGAGATGT GCCCTAGGATTGGACAAAATGATCTTGCTTGGGCACAACCTAGGTGGATTCTTGGCTGCTGCTTACTCGC TGAAGTACCCATCAAGGGTTAATCATCTCATTTTAGTGGAGCCTTGGGGTTTCCCTGAACGACCAGACCT TGCTGATCAAGACAGACCAATTCCAGTTTGGATCAGAGCCTTGGGAGCAGCATTGACTCCCTTTAACCCT TTAGCTGGCCTAAGGATTGCAGGACCCTTTGGTTTAAGTCTAGTGCAGCGTTTAAGGCCTGATTTCAAAC GAAAGTATTCTTCAATGTTCGAAGACGATACTGTGACAGAATACATCTACCACTGTAATGTGCAGACTCC AAGTGGTGAGACAGCTTTCAAGAATATGACTATTCCTTATGGATGGGCAAAAAGGCCAATGCTCCAGCGA ATTGGTAAAATGCACCCTGACATTCCAGTTTCAGTGATCTTTGGCGCCCGATCCTGCATAGATGGCAATT CTGGCACCAGCATCCAGTCCTTACGACCACATTCATATGTGAAGACAATAGCTATTCTTGGGGCAGGACA TTATGTATATGCAGATCAACCAGAAGAATTCAACCAGAAAGTAAAGGAGATCTGCGACACTGTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201869 protein sequence
Red=Cloning site Green=Tags(s) MAAEEEEVDSADTGERSGWLTGWLPTWCPTSISHLKEAEEKMLKCVPCTYKKEPVRISNGNKIWTLKFSH NISNKTPLVLLHGFGGGLGLWALNFGDLCTNRPVYAFDLLGFGRSSRPRFDSDAEEVENQFVESIEEWRC ALGLDKMILLGHNLGGFLAAAYSLKYPSRVNHLILVEPWGFPERPDLADQDRPIPVWIRALGAALTPFNP LAGLRIAGPFGLSLVQRLRPDFKRKYSSMFEDDTVTEYIYHCNVQTPSGETAFKNMTIPYGWAKRPMLQR IGKMHPDIPVSVIFGARSCIDGNSGTSIQSLRPHSYVKTIAILGAGHYVYADQPEEFNQKVKEICDTVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016006 |
| ORF Size | 1047 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016006.6 |
| RefSeq Size | 5370 bp |
| RefSeq ORF | 1050 bp |
| Locus ID | 51099 |
| UniProt ID | Q8WTS1 |
| Domains | abhydrolase |
| Protein Families | Protease |
| MW | 39.1 kDa |
| Gene Summary | The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Microsomal triglyceride transfer protein regulates intracellular lipolysis in adipocytes independent of its lipid transfer activity.
,null,
Metabolism: clinical and experimental
,PubMed ID 36228741
[ABHD5]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201869L1 | Lenti ORF clone of Human abhydrolase domain containing 5 (ABHD5), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201869L2 | Lenti ORF clone of Human abhydrolase domain containing 5 (ABHD5), mGFP tagged |
CNY 5,890.00 |
|
| RC201869L3 | Lenti ORF clone of Human abhydrolase domain containing 5 (ABHD5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201869L4 | Lenti ORF clone of Human abhydrolase domain containing 5 (ABHD5), mGFP tagged |
CNY 5,890.00 |
|
| RG201869 | ABHD5 (tGFP-tagged) - Human abhydrolase domain containing 5 (ABHD5) |
CNY 5,256.00 |
|
| SC114490 | ABHD5 (untagged)-Human abhydrolase domain containing 5 (ABHD5) |
CNY 3,656.00 |
|
| SC322381 | ABHD5 (untagged)-Human abhydrolase domain containing 5 (ABHD5) |
CNY 3,656.00 |
