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alpha 1 Catenin (CTNNA1) (NM_001903) Human Tagged ORF Clone
CAT＃: RC201766
CTNNA1 (Myc-DDK-tagged)-Human catenin (cadherin-associated protein), alpha 1, 102kDa (CTNNA1)
ORF Plasmid: tGFP
"NM_001903" in other vectors (6)
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CNY 6,632.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CAP102; MDPT2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201766 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGCTGTCCATGCAGGCAACATAAACTTCAAGTGGGATCCTAAAAGTCTAGAGATCAGGACTCTGG CAGTTGAGAGACTGTTGGAGCCTCTTGTTACACAGGTTACAACCCTTGTAAACACCAATAGTAAAGGGCC CTCTAATAAGAAGAGAGGTCGTTCTAAGAAGGCCCATGTTTTGGCTGCATCTGTTGAACAAGCAACTGAG AATTTCTTGGAGAAGGGGGATAAAATTGCGAAGGAGAGCCAGTTTCTCAAGGAGGAGCTTGTGGCTGCTG TAGAAGATGTTCGAAAACAAGGTGATTTGATGAAGGCTGCTGCAGGAGAGTTCGCAGATGATCCCTGCTC TTCTGTGAAGCGAGGCAACATGGTTCGGGCAGCTCGAGCTTTGCTCTCTGCTGTTACCCGGTTGCTGATT TTGGCTGACATGGCAGATGTCTACAAATTACTTGTTCAGCTGAAAGTTGTGGAAGATGGTATCTTGAAGT TGAGGAATGCTGGCAATGAACAAGACTTAGGAATCCAGTATAAAGCCCTAAAACCTGAAGTGGATAAGCT GAACATTATGGCAGCCAAAAGACAACAGGAATTGAAAGATGTTGGCCATCGTGATCAGATGGCTGCAGCT AGAGGAATCCTGCAGAAGAACGTTCCGATCCTCTATACTGCATCCCAGGCATGCCTACAGCACCCTGATG TCGCAGCCTATAAGGCCAACAGGGACCTGATATACAAGCAGCTGCAGCAGGCGGTCACAGGCATTTCCAA TGCAGCCCAGGCCACTGCCTCAGACGATGCCTCACAGCACCAGGGTGGAGGAGGAGGAGAACTGGCATAT GCACTCAATAACTTTGACAAACAAATCATTGTGGACCCCTTGAGCTTCAGCGAGGAGCGCTTTAGGCCTT CCCTGGAGGAGCGTCTGGAAAGCATCATTAGTGGGGCTGCCTTGATGGCCGACTCGTCCTGCACGCGTGA TGACCGTCGTGAGCGAATTGTGGCAGAGTGTAATGCTGTCCGCCAGGCCCTGCAGGACCTGCTTTCGGAG TACATGGGCAATGCTGGACGTAAAGAAAGAAGTGATGCACTCAATTCTGCAATAGATAAAATGACCAAGA AGACCAGGGACTTGCGTAGACAGCTCCGCAAAGCTGTCATGGACCACGTTTCAGATTCTTTCCTGGAAAC CAATGTTCCACTTTTGGTATTGATTGAAGCTGCAAAGAATGGAAATGAGAAAGAAGTTAAGGAGTATGCC CAAGTTTTCCGTGAACATGCCAACAAATTGATTGAGGTTGCCAACTTGGCCTGTTCCATCTCAAATAATG AAGAAGGTGTAAAGCTTGTTCGAATGTCTGCAAGCCAGTTAGAAGCCCTCTGTCCTCAGGTTATTAATGC TGCACTGGCTTTAGCAGCAAAACCACAGAGTAAACTGGCCCAAGAGAACATGGATCTTTTTAAAGAACAA TGGGAAAAACAAGTCCGTGTTCTCACAGATGCTGTCGATGACATTACTTCCATTGATGACTTCTTGGCTG TCTCAGAGAATCACATTTTGGAAGATGTGAACAAATGTGTCATTGCTCTCCAAGAGAAGGATGTGGATGG CCTGGACCGCACAGCTGGTGCAATTCGAGGCCGGGCAGCCCGGGTCATTCACGTAGTCACCTCAGAGATG GACAACTATGAGCCAGGAGTCTACACAGAGAAGGTTCTGGAAGCCACTAAGCTGCTCTCCAACACAGTCA TGCCACGTTTTACTGAGCAAGTAGAAGCAGCCGTGGAAGCCCTCAGCTCGGACCCTGCCCAGCCCATGGA TGAGAATGAGTTTATCGATGCTTCCCGCCTGGTATATGATGGCATCCGGGACATCAGGAAAGCAGTGCTG ATGATAAGGACCCCTGAGGAGTTGGATGACTCTGACTTTGAGACAGAAGATTTTGATGTCAGAAGCAGGA CGAGCGTCCAGACAGAAGACGATCAGCTGATAGCTGGCCAGAGTGCCCGGGCGATCATGGCTCAGCTTCC CCAGGAGCAAAAAGCGAAGATTGCGGAACAGGTGGCCAGCTTCCAGGAAGAAAAGAGCAAGCTGGATGCT GAAGTGTCCAAATGGGACGACAGTGGCAATGACATCATTGTGCTGGCCAAGCAGATGTGCATGATTATGA TGGAGATGACAGACTTTACCCGAGGTAAAGGACCACTCAAAAATACATCGGATGTCATCAGTGCTGCCAA GAAAATTGCTGAGGCAGGATCCAGGATGGACAAGCTTGGCCGCACCATTGCAGACCATTGCCCCGACTCG GCTTGCAAGCAGGACCTGCTGGCCTACCTGCAACGCATCGCCCTCTACTGCCACCAGCTGAACATCTGCA GCAAGGTCAAGGCCGAGGTGCAGAATCTCGGCGGGGAGCTTGTTGTCTCTGGGGTGGACAGCGCCATGTC CCTGATCCAGGCAGCCAAGAACTTGATGAATGCTGTGGTGCAGACAGTGAAGGCATCCTACGTCGCCTCT ACCAAATACCAAAAGTCACAGGGTATGGCTTCCCTCAACCTTCCTGCTGTGTCATGGAAGATGAAGGCAC CAGAGAAAAAGCCATTGGTGAAGAGAGAGAAACAGGATGAGACACAGACCAAGATTAAACGGGCATCTCA GAAGAAGCACGTGAACCCGGTGCAGGCCCTCAGCGAGTTCAAAGCTATGGACAGCATC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC201766 protein sequence
Red=Cloning site Green=Tags(s) MTAVHAGNINFKWDPKSLEIRTLAVERLLEPLVTQVTTLVNTNSKGPSNKKRGRSKKAHVLAASVEQATE NFLEKGDKIAKESQFLKEELVAAVEDVRKQGDLMKAAAGEFADDPCSSVKRGNMVRAARALLSAVTRLLI LADMADVYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKALKPEVDKLNIMAAKRQQELKDVGHRDQMAAA RGILQKNVPILYTASQACLQHPDVAAYKANRDLIYKQLQQAVTGISNAAQATASDDASQHQGGGGGELAY ALNNFDKQIIVDPLSFSEERFRPSLEERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSE YMGNAGRKERSDALNSAIDKMTKKTRDLRRQLRKAVMDHVSDSFLETNVPLLVLIEAAKNGNEKEVKEYA QVFREHANKLIEVANLACSISNNEEGVKLVRMSASQLEALCPQVINAALALAAKPQSKLAQENMDLFKEQ WEKQVRVLTDAVDDITSIDDFLAVSENHILEDVNKCVIALQEKDVDGLDRTAGAIRGRAARVIHVVTSEM DNYEPGVYTEKVLEATKLLSNTVMPRFTEQVEAAVEALSSDPAQPMDENEFIDASRLVYDGIRDIRKAVL MIRTPEELDDSDFETEDFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDA EVSKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDS ACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVAS TKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEFKAMDSI SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001903 |
| ORF Size | 2718 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001903.5 |
| RefSeq Size | 3791 bp |
| RefSeq ORF | 2721 bp |
| Locus ID | 1495 |
| UniProt ID | P35221 |
| Domains | Vinculin |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Endometrial cancer, Leukocyte transendothelial migration, Pathways in cancer, Tight junction |
| MW | 100.1 kDa |
| Gene Summary | This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201766L1 | Lenti ORF clone of Human catenin (cadherin-associated protein), alpha 1, 102kDa (CTNNA1), Myc-DDK-tagged |
CNY 9,032.00 |
|
| RC201766L2 | Lenti ORF clone of Human catenin (cadherin-associated protein), alpha 1, 102kDa (CTNNA1), mGFP tagged |
CNY 5,890.00 |
|
| RC201766L3 | Lenti ORF clone of Human catenin (cadherin-associated protein), alpha 1, 102kDa (CTNNA1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201766L4 | Lenti ORF clone of Human catenin (cadherin-associated protein), alpha 1, 102kDa (CTNNA1), mGFP tagged |
CNY 9,032.00 |
|
| RG201766 | CTNNA1 (tGFP-tagged) - Human catenin (cadherin-associated protein), alpha 1, 102kDa (CTNNA1) |
CNY 8,232.00 |
|
| SC118935 | CTNNA1 (untagged)-Human catenin (cadherin-associated protein), alpha 1, 102kDa (CTNNA1) |
CNY 6,640.00 |
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