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Chromogranin B (CHGB) (NM_001819) Human Tagged ORF Clone
CAT＃: RC201744
- TrueORF®
CHGB (Myc-DDK-tagged)-Human chromogranin B (secretogranin 1) (CHGB)
ORF Plasmid: tGFP
"NM_001819" in other vectors (6)
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CNY 7,440.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SCG1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201744 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCAACGCTGCTTCTCAGCCTCCTGGGAGCCGTGGGGCTGGCGGCTGTCAATTCCATGCCAGTGG ATAACAGGAACCACAATGAAGGAATGGTGACTCGCTGCATCATTGAGGTCCTCTCAAATGCCTTGTCGAA GTCCAGCGCTCCACCCATCACCCCTGAGTGCCGCCAAGTCCTGAAGACGAGTAGAAAAGACGTCAAAGAC AAAGAGACAACTGAAAATGAAAACACAAAGTTTGAAGTAAGATTGTTAAGAGACCCAGCTGATGCCTCGG AAGCCCACGAGTCCTCCAGCAGGGGAGAGGCAGGAGCCCCAGGGGAGGAGGACATCCAAGGCCCAACAAA GGCAGACACAGAGAAATGGGCAGAGGGAGGCGGGCACAGCCGAGAGCGAGCGGATGAGCCCCAGTGGAGC CTCTATCCCTCCGACAGCCAAGTCTCTGAAGAAGTGAAGACACGCCATTCTGAGAAGAGCCAGAGAGAGG ATGAGGAGGAGGAGGAGGGAGAGAACTATCAAAAAGGGGAGCGAGGGGAAGATAGCAGTGAAGAGAAACA CCTTGAAGAGCCAGGAGAGACACAAAACGCTTTTCTCAATGAAAGAAAGCAGGCTTCAGCTATAAAAAAA GAGGAGTTAGTGGCCAGATCGGAAACACATGCTGCCGGGCATTCTCAGGAGAAGACACATAGCCGAGAGA AGAGTAGCCAGGAGAGTGGAGAGGAGGCAGGGAGCCAGGAGAATCACCCCCAGGAGTCTAAAGGCCAACC CCGAAGCCAGGAAGAATCTGAGGAAGGTGAGGAAGATGCCACCTCTGAGGTGGACAAACGACGCACGAGG CCCAGACACCACCACGGGAGGAGCAGGCCCGACAGGTCCTCTCAAGGAGGGAGTCTTCCCTCTGAGGAAA AGGGACACCCCCAGGAGGAATCTGAGGAGTCAAACGTCAGCATGGCCAGTTTAGGGGAAAAGAGGGACCA CCATTCAACCCACTACAGGGCTTCAGAGGAAGAACCTGAATATGGAGAAGAAATAAAGGGTTATCCAGGC GTCCAGGCCCCTGAGGACCTGGAGTGGGAGCGCTATAGGGGCAGAGGAAGTGAAGAATACAGGGCTCCAA GACCTCAGAGTGAGGAGAGTTGGGATGAGGAGGACAAGAGAAACTACCCCAGCTTAGAGCTTGATAAGAT GGCACATGGATATGGTGAAGAAAGTGAGGAAGAGAGGGGCCTTGAGCCGGGAAAGGGACGCCATCACAGA GGCAGGGGAGGGGAGCCACGTGCCTATTTCATGTCTGACACCAGAGAAGAGAAAAGGTTCTTGGGTGAAG GACACCACCGTGTCCAAGAAAACCAGATGGACAAGGCAAGGAGGCATCCACAAGGTGCGTGGAAAGAGCT GGACAGAAATTATCTCAACTACGGTGAGGAAGGAGCCCCAGGGAAGTGGCAGCAGCAGGGAGACCTGCAG GACACTAAAGAAAACAGGGAGGAAGCTAGGTTTCAAGATAAACAATATAGCTCCCATCACACAGCTGAAA AGAGGAAGAGATTAGGGGAACTGTTCAACCCATACTACGACCCTCTCCAGTGGAAGAGCAGCCATTTTGA AAGAAGAGACAACATGAATGACAATTTTCTCGAGGGTGAGGAGGAAAATGAGCTGACCTTGAACGAGAAG AATTTCTTCCCAGAATACAACTATGACTGGTGGGAGAAAAAGCCCTTCTCTGAGGATGTGAACTGGGGGT ATGAGAAGAGAAACCTCGCCAGGGTCCCCAAGCTGGACCTGAAAAGGCAATATGACAGGGTGGCCCAACT GGACCAGCTCCTTCACTACAGGAAGAAGTCAGCTGAGTTTCCAGACTTCTATGATTCTGAGGAGCCGGTG AGCACCCACCAGGAGGCAGAAAATGAAAAGGACAGGGCTGACCAGACAGTCCTGACAGAGGACGAGAAAA AAGAACTCGAAAACTTGGCTGCAATGGATTTGGAACTACAGAAGATAGCTGAGAAATTCAGCCAAAGGGG C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201744 protein sequence
Red=Cloning site Green=Tags(s) MQPTLLLSLLGAVGLAAVNSMPVDNRNHNEGMVTRCIIEVLSNALSKSSAPPITPECRQVLKTSRKDVKD KETTENENTKFEVRLLRDPADASEAHESSSRGEAGAPGEEDIQGPTKADTEKWAEGGGHSRERADEPQWS LYPSDSQVSEEVKTRHSEKSQREDEEEEEGENYQKGERGEDSSEEKHLEEPGETQNAFLNERKQASAIKK EELVARSETHAAGHSQEKTHSREKSSQESGEEAGSQENHPQESKGQPRSQEESEEGEEDATSEVDKRRTR PRHHHGRSRPDRSSQGGSLPSEEKGHPQEESEESNVSMASLGEKRDHHSTHYRASEEEPEYGEEIKGYPG VQAPEDLEWERYRGRGSEEYRAPRPQSEESWDEEDKRNYPSLELDKMAHGYGEESEEERGLEPGKGRHHR GRGGEPRAYFMSDTREEKRFLGEGHHRVQENQMDKARRHPQGAWKELDRNYLNYGEEGAPGKWQQQGDLQ DTKENREEARFQDKQYSSHHTAEKRKRLGELFNPYYDPLQWKSSHFERRDNMNDNFLEGEEENELTLNEK NFFPEYNYDWWEKKPFSEDVNWGYEKRNLARVPKLDLKRQYDRVAQLDQLLHYRKKSAEFPDFYDSEEPV STHQEAENEKDRADQTVLTEDEKKELENLAAMDLELQKIAEKFSQRG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001819 |
| ORF Size | 2031 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001819.1, NP_001810.1 |
| RefSeq Size | 2666 bp |
| RefSeq ORF | 2034 bp |
| Locus ID | 1114 |
| UniProt ID | P05060 |
| Domains | Granin |
| Protein Families | Secreted Protein |
| MW | 78.2 kDa |
| Gene Summary | This gene encodes a tyrosine-sulfated secretory protein abundant in peptidergic endocrine cells and neurons. This protein may serve as a precursor for regulatory peptides. [provided by RefSeq, Jan 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201744L1 | Lenti ORF clone of Human chromogranin B (secretogranin 1) (CHGB), Myc-DDK-tagged |
CNY 9,840.00 |
|
| RC201744L2 | Lenti ORF clone of Human chromogranin B (secretogranin 1) (CHGB), mGFP tagged |
CNY 5,890.00 |
|
| RC201744L3 | Lenti ORF clone of Human chromogranin B (secretogranin 1) (CHGB), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201744L4 | Lenti ORF clone of Human chromogranin B (secretogranin 1) (CHGB), mGFP tagged |
CNY 5,890.00 |
|
| RG201744 | CHGB (tGFP-tagged) - Human chromogranin B (secretogranin 1) (CHGB) |
CNY 9,040.00 |
|
| SC119004 | CHGB (untagged)-Human chromogranin B (secretogranin 1) (CHGB) |
CNY 7,448.00 |
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