CIRBP (NM_001280) Human Tagged ORF Clone
CAT#: RC201639
CIRBP (Myc-DDK-tagged)-Human cold inducible RNA binding protein (CIRBP), transcript variant 1
ORF Plasmid: tGFP
"NM_001280" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CIRP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201639 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCAGATGAAGGCAAACTTTTTGTTGGAGGGCTGAGTTTTGACACCAATGAGCAGTCGCTGGAGC AGGTCTTCTCAAAGTACGGACAGATCTCTGAAGTGGTGGTTGTGAAAGACAGGGAGACCCAGAGATCTCG GGGATTTGGGTTTGTCACCTTTGAGAACATTGACGACGCTAAGGATGCCATGATGGCCATGAATGGGAAG TCTGTAGATGGACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCCCGTGGGTACC GTGGTGGCTCTGCCGGGGGCCGGGGCTTCTTCCGTGGGGGCCGAGGACGGGGCCGTGGGTTCTCTAGAGG AGGAGGGGACCGAGGCTATGGGGGGAACCGGTTCGAGTCCAGGAGTGGGGGCTACGGAGGCTCCAGAGAC TACTATAGCAGCCGGAGTCAGAGTGGTGGCTACAGTGACCGGAGCTCGGGCGGGTCCTACAGAGACAGTT ACGACAGTTACGCTACACACAACGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201639 protein sequence
Red=Cloning site Green=Tags(s) MASDEGKLFVGGLSFDTNEQSLEQVFSKYGQISEVVVVKDRETQRSRGFGFVTFENIDDAKDAMMAMNGK SVDGRQIRVDQAGKSSDNRSRGYRGGSAGGRGFFRGGRGRGRGFSRGGGDRGYGGNRFESRSGGYGGSRD YYSSRSQSGGYSDRSSGGSYRDSYDSYATHNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001280 |
ORF Size | 516 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001280.1, NP_001271.1 |
RefSeq Size | 1397 bp |
RefSeq ORF | 519 bp |
Locus ID | 1153 |
UniProt ID | Q14011 |
Domains | RRM, RRM_1 |
MW | 18.6 kDa |
Gene Summary | Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3' UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Extracellular CIRP Activates the IL-6Rα/STAT3/Cdk5 Pathway in Neurons
,Sharma, A;Brenner, M;Jacob, A;Marambaud, P;Wang, P;,
Research Square
[CIRBP]
|
Extracellular CIRP induces macrophage endotoxin tolerance through IL-6R–mediated STAT3 activation
,null,
JCI Insight
,PubMed ID 32027619
[CIRBP]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201639L1 | Lenti ORF clone of Human cold inducible RNA binding protein (CIRBP), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC201639L2 | Lenti ORF clone of Human cold inducible RNA binding protein (CIRBP), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC201639L3 | Lenti ORF clone of Human cold inducible RNA binding protein (CIRBP), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201639L4 | Lenti ORF clone of Human cold inducible RNA binding protein (CIRBP), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
RG201639 | CIRBP (tGFP-tagged) - Human cold inducible RNA binding protein (CIRBP), transcript variant 1 |
CNY 4,000.00 |
|
SC119359 | CIRBP (untagged)-Human cold inducible RNA binding protein (CIRBP), transcript variant 1 |
CNY 2,400.00 |