Importin 13 (IPO13) (NM_014652) Human Tagged ORF Clone

CAT#: RC201577

IPO13 (Myc-DDK-tagged)-Human importin 13 (IPO13)

ORF Plasmid: DDK tGFP



  "NM_014652" in other vectors (7)

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CNY 10,576.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal antibody to Importin 13 (importin 13)
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms IMP13; KAP13; LGL2; RANBP13
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201577 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGGCGGGAGGAGCAGCCGGGGGCTGCAGGGGCTGGAGCAGCACCAGCCTTGGACTTCACTGTGG
AGAACGTGGAGAAGGCGCTGCACCAGCTCTACTATGATCCCAACATTGAGAATAAGAACCTGGCTCAGAA
GTGGCTGATGCAGGCCCAGGTCTCCCCACAGGCCTGGCACTTCAGCTGGCAGCTACTGCAGCCCGACAAG
GTACCAGAGATCCAGTACTTTGGGGCCAGTGCTCTTCACATCAAGATCTCTCGCTACTGGAGTGACATCC
CCACTGACCAGTATGAAAGCCTAAAGGCACAGCTCTTCACCCAGATCACCCGCTTTGCCAGTGGCTCCAA
GATTGTACTGACTCGGCTGTGCGTGGCACTGGCCTCACTGGCTCTCAGCATGATGCCTGATGCTTGGCCA
TGTGCTGTGGCAGATATGGTACGACTCTTCCAGGCTGAGGACTCACCAGTGGATGGGCAGGGCCGCTGCC
TAGCCCTGTTAGAGCTGCTGACAGTGCTGCCTGAGGAGTTCCAGACCAGTCGCCTACCCCAGTACCGCAA
AGGCCTGGTGCGGACCAGCCTGGCGGTGGAATGTGGGGCTGTCTTCCCGCTGCTGGAGCAGCTGCTACAG
CAGCCCAGCTCACCCAGCTGTGTGCGTCAGAAGGTGCTCAAGTGTTTCTCCAGCTGGGTGCAGCTGGAGG
TGCCGCTGCAGGACTGTGAGGCGCTCATTCAGGCTGCCTTTGCTGCTCTGCAGGACTCGGAGCTCTTCGA
CAGCAGTGTGGAGGCCATTGTGAATGCCATCTCACAGCCTGATGCCCAGAGGTACGTGAACACACTCCTG
AAACTCATCCCGCTGGTGCTGGGTCTGCAGGAACAACTGCGGCAGGCAGTGCAGAATGGGGACATGGAGA
CCTCCCATGGCATCTGTCGCATCGCTGTGGCCCTGGGCGAGAACCACTCCCGGGCCTTGCTGGACCAAGT
AGAGCACTGGCAGAGTTTCCTGGCACTCGTCAACATGATTATGTTCTGCACAGGCATCCCTGGCCACTAT
CCTGTCAATGAGACCACCAGCTCCCTAACCCTCACCTTCTGGTACACACTGCAGGATGATATTCTATCCT
TTGAGGCAGAGAAGCAGGCTGTATACCAGCAGGTGTACCGGCCAGTCTACTTCCAGCTGGTGGATGTGCT
TCTGCACAAGGCCCAGTTCCCTTCTGATGAGGAATATGGATTCTGGTCCTCAGACGAGAAGGAGCAGTTC
CGAATTTACAGGGTGGACATCTCAGACACGCTCATGTATGTCTATGAGATGTTGGGGGCCGAGCTGCTCA
GCAACCTCTATGACAAGCTGGGTCGTTTGCTCACCAGCTCAGAGGAGCCCTACTCCTGGCAGCACACAGA
GGCCCTCCTCTACGGCTTCCAATCCATCGCAGAGACCATTGACGTCAACTATTCTGATGTGGTGCCTGGG
CTCATTGGCCTCATCCCACGGATCAGCATCAGCAACGTGCAGCTGGCAGACACTGTCATGTTCACCATTG
GAGCTCTGTCTGAATGGCTGGCTGACCACCCCGTCATGATCAACAGTGTTCTGCCCTTGGTACTGCATGC
CCTAGGCAATCCTGAGCTGTCTGTCTCTTCTGTGTCCACCCTCAAGAAGATCTGCCGAGAGTGCAAGTAT
GACCTGCCTCCCTATGCTGCCAACATTGTGGCTGTGTCCCAGGATGTGCTGATGAAACAGATCCACAAGA
CAAGCCAGTGCATGTGGCTGATGCAGGCGCTGGGCTTCCTGCTGTCAGCTCTTCAAGTGGAGGAGATCCT
TAAGAACCTGCACTCGCTTATCTCACCCTATATCCAGCAACTGGAGAAGCTGGCAGAGGAGATACCCAAT
CCCTCCAACAAGCTGGCCATTGTTCACATCTTGGGGCTTCTCTCCAACCTCTTCACCACACTGGACATCA
GTCATCATGAGGATGATCATGAAGGCCCTGAGCTTCGGAAGCTGCCAGTGCCACAGGGACCCAACCCCGT
GGTGGTGGTGCTGCAGCAGGTCTTCCAGCTTATCCAGAAGGTGCTGAGCAAATGGTTGAATGATGCCCAG
GTTGTGGAGGCGGTGTGCGCTATCTTTGAGAAGTCTGTTAAGACGCTGCTGGATGACTTTGCCCCCATGG
TGCCACAGCTGTGTGAGATGCTGGGTCGGATGTACAGCACCATCCCCCAGGCCTCTGCTCTTGACCTCAC
TCGACAGCTGGTCCACATCTTTGCTCATGAGCCTGCCCACTTTCCCCCAATTGAGGCCCTCTTCCTGCTC
GTCACCTCCGTCACACTCACTCTCTTCCAGCAAGGGCCCAGGGATCATCCTGATATTGTTGATTCATTTA
TGCAACTCCTGGCACAGGCTCTGAAGCGGAAGCCAGATTTGTTCCTGTGTGAACGATTGGATGTCAAATC
TGTGTTCCAGTGTGCTGTGCTGGCCCTCAAGTTCCCTGAGGCACCTACTGTCAAGGCCTCCTGTGGCTTC
TTTACAGAGCTGCTGCCTCGGTGTGGGGAAGTAGAGTCTGTGGGAAAGGTGGTACAGGAAGACGGTCGTA
TGCTGCTCATAGCAGTGCTGGAGGCCATTGGGGGCCAGGCCTCCCGCAGCCTCATGGACTGCTTTGCCGA
TATCCTGTTCGCCCTGAACAAGCACTGCTTCAGCCTCCTGAGCATGTGGATCAAGGAGGCCCTGCAGCCA
CCTGGTTTCCCCTCTGCCCGCCTCAGCCCTGAACAGAAGGATACCTTCAGCCAGCAGATCCTTCGCGAGC
GAGTGAACAAGAGGCGGGTGAAGGAGATGGTGAAGGAGTTCACACTGCTGTGCCGGGGTCTCCATGGCAC
AGATTACACAGCTGACTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201577 protein sequence
Red=Cloning site Green=Tags(s)

MERREEQPGAAGAGAAPALDFTVENVEKALHQLYYDPNIENKNLAQKWLMQAQVSPQAWHFSWQLLQPDK
VPEIQYFGASALHIKISRYWSDIPTDQYESLKAQLFTQITRFASGSKIVLTRLCVALASLALSMMPDAWP
CAVADMVRLFQAEDSPVDGQGRCLALLELLTVLPEEFQTSRLPQYRKGLVRTSLAVECGAVFPLLEQLLQ
QPSSPSCVRQKVLKCFSSWVQLEVPLQDCEALIQAAFAALQDSELFDSSVEAIVNAISQPDAQRYVNTLL
KLIPLVLGLQEQLRQAVQNGDMETSHGICRIAVALGENHSRALLDQVEHWQSFLALVNMIMFCTGIPGHY
PVNETTSSLTLTFWYTLQDDILSFEAEKQAVYQQVYRPVYFQLVDVLLHKAQFPSDEEYGFWSSDEKEQF
RIYRVDISDTLMYVYEMLGAELLSNLYDKLGRLLTSSEEPYSWQHTEALLYGFQSIAETIDVNYSDVVPG
LIGLIPRISISNVQLADTVMFTIGALSEWLADHPVMINSVLPLVLHALGNPELSVSSVSTLKKICRECKY
DLPPYAANIVAVSQDVLMKQIHKTSQCMWLMQALGFLLSALQVEEILKNLHSLISPYIQQLEKLAEEIPN
PSNKLAIVHILGLLSNLFTTLDISHHEDDHEGPELRKLPVPQGPNPVVVVLQQVFQLIQKVLSKWLNDAQ
VVEAVCAIFEKSVKTLLDDFAPMVPQLCEMLGRMYSTIPQASALDLTRQLVHIFAHEPAHFPPIEALFLL
VTSVTLTLFQQGPRDHPDIVDSFMQLLAQALKRKPDLFLCERLDVKSVFQCAVLALKFPEAPTVKASCGF
FTELLPRCGEVESVGKVVQEDGRMLLIAVLEAIGGQASRSLMDCFADILFALNKHCFSLLSMWIKEALQP
PGFPSARLSPEQKDTFSQQILRERVNKRRVKEMVKEFTLLCRGLHGTDYTADY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014652
ORF Size 2889 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014652.2, NP_055467.2
RefSeq Size 4041 bp
RefSeq ORF 2892 bp
Locus ID 9670
UniProt ID O94829
Domains IBN_NT
MW 108.2 kDa
Gene Summary This gene encodes a member of the importin-beta family of nuclear transport proteins. The encoded protein mediates the import of specific cargo proteins from the cytoplasm to the nucleus and is dependent on the Ras-related nuclear protein-GTPase system. The encoded protein is also involved in nuclear export of the eukaryotic translation initiation factor 1A.[provided by RefSeq, Mar 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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