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Ribophorin I (RPN1) (NM_002950) Human Tagged ORF Clone
CAT＃: RC201554
RPN1 (Myc-DDK-tagged)-Human ribophorin I (RPN1)
ORF Plasmid: tGFP
"NM_002950" in other vectors (5)
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CNY 4,520.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | OST1; RBPH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201554 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGCCAGCCGCCGGCTTGTTTCTGCTCCTGTTGCTTGGGACTTGGGCCCCGGCGCCGGGCAGCG CCTCCTCCGAGGCACCGCCGCTGATCAATGAGGACGTGAAGCGCACAGTGGACCTAAGCAGCCACCTGGC TAAGGTGACGGCCGAGGTGGTCCTGGCGCACCTGGGCGGCGGCTCCACGTCCCGAGCTACCTCTTTCCTG CTGGCTTTGGAGCCTGAGCTCGAGGCCCGGCTGGCGCACCTGGGCGTGCAGGTAAAGGGAGAAGATGAGG AAGAGAACAATTTGGAAGTACGTGAAACCAAAATTAAGGGTAAAAGTGGGAGATTCTTCACAGTCAAGCT CCCAGTTGCTCTTGATCCTGGGGCCAAGATTTCAGTCATTGTGGAAACAGTCTACACCCATGTGCTTCAT CCATATCCAACCCAGATCACCCAGTCAGAGAAACAGTTTGTGGTGTTTGAGGGGAACCATTATTTCTACT CTCCCTATCCAACGAAGACACAAACCATGCGTGTGAAGCTTGCCTCTCGAAATGTGGAGAGCTACACCAA GCTGGGGAACCCCACGCGCTCTGAGGACCTACTGGATTATGGGCCTTTCAGAGATGTGCCTGCCTATAGT CAGGATACTTTTAAAGTACATTATGAGAACAACAGCCCTTTCCTGACCATCACCAGCATGACCCGAGTCA TTGAAGTCTCTCACTGGGGTAATATTGCTGTGGAAGAAAATGTGGACTTAAAGCACACAGGAGCTGTGCT TAAGGGGCCTTTCTCACGCTATGATTACCAGAGACAGCCAGATAGTGGAATATCCTCCATCCGTTCTTTT AAGACCATCCTTCCTGCTGCTGCCCAGGATGTTTATTACCGGGATGAGATTGGCAATGTTTCTACCAGCC ACCTCCTTATTTTGGATGACTCTGTAGAGATGGAAATCCGGCCTCGCTTCCCTCTCTTTGGCGGGTGGAA GACCCATTACATCGTTGGCTACAACCTCCCAAGCTATGAGTACCTCTATAATTTGGGTGACCAGTATGCA CTGAAGATGAGGTTTGTGGACCATGTGTTTGATGAACAAGTGATAGATTCTCTGACTGTGAAGATCATCC TGCCTGAAGGAGCCAAGAACATTGAAATTGATAGTCCCTATGAAATCAGCCGTGCCCCAGATGAGCTGCA CTACACCTATCTGGATACATTTGGCCGCCCTGTGATTGTTGCCTACAAGAAAAATCTGGTAGAACAGCAC ATTCAGGACATTGTGGTCCACTACACGTTCAACAAGGTGCTCATGCTGCAGGAGCCCCTGCTGGTGGTGG CGGCCTTCTACATCCTGTTCTTCACCGTTATCATCTATGTTCGGCTGGACTTCTCCATCACCAAGGATCC AGCCGCAGAAGCCAGGATGAAGGTAGCCTGCATCACAGAGCAGGTCTTGACCCTGGTCAACAAGAGAATA GGCCTTTACCGTCACTTTGACGAGACCGTCAATAGGTACAAGCAATCCCGGGACATCTCCACCCTCAACA GTGGCAAGAAGAGCCTGGAGACTGAACACAAGGCCTTGACCAGTGAGATTGCACTGCTGCAGTCCAGGCT GAAGACAGAGGGCTCTGATCTGTGCGACAGAGTGAGCGAAATGCAGAAGCTGGATGCACAGGTCAAGGAG CTGGTGCTGAAGTCGGCGGTGGAGGCTGAGCGCCTGGTGGCTGGCAAGCTCAAGAAAGACACGTACATTG AGAATGAGAAGCTCATCTCAGGAAAGCGCCAGGAGCTGGTCACCAAGATCGACCACATCCTGGATGCCCT G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201554 protein sequence
Red=Cloning site Green=Tags(s) MEAPAAGLFLLLLLGTWAPAPGSASSEAPPLINEDVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFL LALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLH PYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYS QDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSF KTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGYNLPSYEYLYNLGDQYA LKMRFVDHVFDEQVIDSLTVKIILPEGAKNIEIDSPYEISRAPDELHYTYLDTFGRPVIVAYKKNLVEQH IQDIVVHYTFNKVLMLQEPLLVVAAFYILFFTVIIYVRLDFSITKDPAAEARMKVACITEQVLTLVNKRI GLYRHFDETVNRYKQSRDISTLNSGKKSLETEHKALTSEIALLQSRLKTEGSDLCDRVSEMQKLDAQVKE LVLKSAVEAERLVAGKLKKDTYIENEKLISGKRQELVTKIDHILDAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002950 |
| ORF Size | 1821 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002950.2 |
| RefSeq Size | 2372 bp |
| RefSeq ORF | 1824 bp |
| Locus ID | 6184 |
| UniProt ID | P04843 |
| Domains | Ribophorin_I |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| MW | 68.6 kDa |
| Gene Summary | This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Cholix protein domain I functions as a carrier element for efficient apical to basal epithelial transcytosis
,Taverner, A;MacKay, J;Laurent, F;Hunter, T;Liu, K;Mangat, K;Song, L;Seto, E;Postlethwaite, S;Alam, A;Chandalia, A;Seung, M;Saberi, M;Feng, W;Mrsny, RJ;,
Tissue Barriers
,PubMed ID 31928299
[Ribophorin I]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201554L3 | Lenti ORF clone of Human ribophorin I (RPN1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201554L4 | Lenti ORF clone of Human ribophorin I (RPN1), mGFP tagged |
CNY 5,890.00 |
|
| RG201554 | RPN1 (tGFP-tagged) - Human ribophorin I (RPN1) |
CNY 6,120.00 |
|
| SC118293 | RPN1 (untagged)-Human ribophorin I (RPN1) |
CNY 4,528.00 |
|
| SC324267 | RPN1 (untagged)-Human ribophorin I (RPN1) |
CNY 4,528.00 |
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