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NFIL3 (NM_005384) Human Tagged ORF Clone
CAT＃: RC201537
NFIL3 (Myc-DDK-tagged)-Human nuclear factor, interleukin 3 regulated (NFIL3)
ORF Plasmid: tGFP
"NM_005384" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | E4BP4; IL3BP1; NF-IL3A; NFIL3A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201537 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCTGAGAAAAATGCAGACCGTCAAAAAGGAGCAGGCGTCTCTTGATGCCAGTAGCAATGTGGACA AGATGATGGTCCTTAATTCTGCTTTAACGGAAGTGTCAGAAGACTCCACAACAGGTGAGGAGCTGCTTCT CAGTGAAGGAAGTGTGGGGAAGAACAAATCTTCTGCATGTCGGAGGAAACGGGAATTCATTCCTGATGAA AAGAAAGATGCTATGTATTGGGAAAAAAGGCGGAAAAATAATGAAGCTGCCAAAAGATCTCGTGAGAAGC GTCGACTGAATGACCTGGTTTTAGAGAACAAACTAATTGCACTGGGAGAAGAAAACGCCACTTTAAAAGC TGAGCTGCTTTCACTAAAATTAAAGTTTGGTTTAATTAGCTCCACAGCATATGCTCAAGAGATTCAGAAA CTCAGTAATTCTACAGCTGTGTACTTTCAAGATTACCAGACTTCCAAATCCAATGTGAGTTCATTTGTGG ACGAGCACGAACCCTCGATGGTGTCAAGTAGTTGTATTTCTGTCATTAAACACTCTCCACAAAGCTCGCT GTCCGATGTTTCAGAAGTGTCCTCAGTAGAACACACGCAGGAGAGCTCTGTGCAGGGAAGCTGCAGAAGT CCTGAAAACAAGTTCCAGATTATCAAGCAAGAGCCGATGGAATTAGAGAGCTACACAAGGGAGCCAAGAG ATGACCGAGGCTCTTACACAGCGTCCATCTATCAAAACTATATGGGGAATTCTTTCTCTGGGTACTCACA CTCTCCCCCACTACTGCAAGTCAACCGATCCTCCAGCAACTCCCCGAGAACGTCGGAAACTGATGATGGT GTGGTAGGAAAGTCATCTGATGGAGAAGACGAGCAACAGGTCCCCAAGGGCCCCATCCATTCTCCAGTTG AACTCAAGCATGTGCATGCAACTGTGGTTAAAGTTCCAGAAGTGAATTCCTCTGCCTTGCCACACAAGCT CCGGATCAAAGCCAAAGCCATGCAGATCAAAGTAGAAGCCTTTGATAATGAATTTGAGGCCACGCAAAAA CTTTCCTCACCTATTGACATGACATCTAAAAGACATTTCGAACTCGAAAAGCATAGTGCCCCAAGTATGG TACATTCTTCTCTTACTCCTTTCTCAGTGCAAGTGACTAACATTCAAGATTGGTCTCTCAAATCGGAGCA CTGGCATCAAAAAGAACTGAGTGGCAAAACTCAGAATAGTTTCAAAACTGGAGTTGTTGAAATGAAAGAC AGTGGCTACAAAGTTTCTGACCCAGAGAACTTGTATTTGAAGCAGGGGATAGCAAACTTATCTGCAGAGG TTGTCTCACTCAAGAGACTTATAGCCACACAACCAATCTCTGCTTCAGACTCTGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201537 protein sequence
Red=Cloning site Green=Tags(s) MQLRKMQTVKKEQASLDASSNVDKMMVLNSALTEVSEDSTTGEELLLSEGSVGKNKSSACRRKREFIPDE KKDAMYWEKRRKNNEAAKRSREKRRLNDLVLENKLIALGEENATLKAELLSLKLKFGLISSTAYAQEIQK LSNSTAVYFQDYQTSKSNVSSFVDEHEPSMVSSSCISVIKHSPQSSLSDVSEVSSVEHTQESSVQGSCRS PENKFQIIKQEPMELESYTREPRDDRGSYTASIYQNYMGNSFSGYSHSPPLLQVNRSSSNSPRTSETDDG VVGKSSDGEDEQQVPKGPIHSPVELKHVHATVVKVPEVNSSALPHKLRIKAKAMQIKVEAFDNEFEATQK LSSPIDMTSKRHFELEKHSAPSMVHSSLTPFSVQVTNIQDWSLKSEHWHQKELSGKTQNSFKTGVVEMKD SGYKVSDPENLYLKQGIANLSAEVVSLKRLIATQPISASDSG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005384 |
| ORF Size | 1386 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005384.3 |
| RefSeq Size | 2104 bp |
| RefSeq ORF | 1389 bp |
| Locus ID | 4783 |
| UniProt ID | Q16649 |
| Domains | BRLZ |
| Protein Families | Transcription Factors |
| MW | 51.5 kDa |
| Gene Summary | The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201537L1 | Lenti ORF clone of Human nuclear factor, interleukin 3 regulated (NFIL3), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201537L2 | Lenti ORF clone of Human nuclear factor, interleukin 3 regulated (NFIL3), mGFP tagged |
CNY 5,890.00 |
|
| RC201537L3 | Lenti ORF clone of Human nuclear factor, interleukin 3 regulated (NFIL3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201537L4 | Lenti ORF clone of Human nuclear factor, interleukin 3 regulated (NFIL3), mGFP tagged |
CNY 5,890.00 |
|
| RG201537 | NFIL3 (tGFP-tagged) - Human nuclear factor, interleukin 3 regulated (NFIL3) |
CNY 5,256.00 |
|
| SC116782 | NFIL3 (untagged)-Human nuclear factor, interleukin 3 regulated (NFIL3) |
CNY 3,656.00 |
