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SGTA (NM_003021) Human Tagged ORF Clone
CAT＃: RC201429
SGTA (Myc-DDK-tagged)-Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA)
ORF Plasmid: tGFP
"NM_003021" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
| Cited in 3 publications. |
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | alphaSGT; hSGT; SGT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201429 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAACAAGAAGCGCCTGGCCTACGCCATCATCCAGTTCCTGCATGACCAGCTCCGGCACGGGGGCC TCTCGTCCGATGCTCAGGAGAGCTTGGAAGTCGCCATCCAGTGCCTGGAGACTGCGTTTGGGGTGACGGT AGAAGACAGTGACCTTGCGCTCCCTCAGACTCTGCCGGAGATATTTGAAGCGGCTGCCACGGGCAAGGAG ATGCCGCAGGACCTGAGGAGCCCCGCGCGAACCCCGCCTTCCGAGGAGGACTCAGCAGAGGCAGAGCGCC TCAAAACCGAAGGAAACGAGCAGATGAAAGTGGAAAACTTTGAAGCTGCCGTGCATTTCTACGGAAAAGC CATCGAGCTCAACCCAGCCAACGCCGTCTATTTCTGCAACAGAGCCGCAGCCTACAGCAAACTCGGCAAC TACGCAGGCGCGGTGCAGGACTGTGAGCGGGCCATCTGCATTGACCCGGCCTACAGCAAGGCCTACGGCA GGATGGGCCTGGCGCTCTCCAGCCTCAACAAGCACGTGGAGGCCGTGGCTTACTACAAGAAGGCTCTGGA GCTGGACCCCGACAACGAGACATACAAGTCCAACCTCAAGATAGCGGAGCTGAAGCTGCGGGAGGCCCCC AGCCCCACGGGAGGCGTGGGCAGCTTCGACATCGCCGGCCTGCTGAACAACCCTGGCTTCATGAGCATGG CTTCGAACCTAATGAACAATCCCCAGATTCAGCAGCTCATGTCCGGCATGATTTCGGGTGGCAACAACCC CTTGGGAACTCCCGGCACCAGCCCCTCGCAGAACGACCTGGCCAGCCTCATCCAGGCGGGCCAGCAGTTT GCCCAGCAGATGCAGCAGCAGAACCCAGAGTTGATAGAGCAGCTCAGGAGCCAGATCCGGAGTCGGACGC CCAGCGCCAGCAACGACGACCAGCAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201429 protein sequence
Red=Cloning site Green=Tags(s) MDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKE MPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIELNPANAVYFCNRAAAYSKLGN YAGAVQDCERAICIDPAYSKAYGRMGLALSSLNKHVEAVAYYKKALELDPDNETYKSNLKIAELKLREAP SPTGGVGSFDIAGLLNNPGFMSMASNLMNNPQIQQLMSGMISGGNNPLGTPGTSPSQNDLASLIQAGQQF AQQMQQQNPELIEQLRSQIRSRTPSASNDDQQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003021 |
| ORF Size | 939 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003021.4 |
| RefSeq Size | 2358 bp |
| RefSeq ORF | 942 bp |
| Locus ID | 6449 |
| UniProt ID | O43765 |
| Domains | TPR |
| Protein Families | Druggable Genome |
| MW | 34.1 kDa |
| Gene Summary | This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
SGTA-dependent regulation of Hsc70 promotes cytosol entry of SV40 from the ER
,Dupzyk, A;Williams, JM;Bagchi, P;Inoue, T;Tsai, B;,
J. Virol.
,PubMed ID 28356524
[SGTA]
|
|
A Cytosolic Chaperone Complexes with Dynamic Membrane J-Proteins and Mobilizes a Nonenveloped Virus out of the Endoplasmic Reticulum
,Walczak, CP;Ravindran, MS;Inoue, T;Tsai, B;,
PLoS Pathog.
,PubMed ID 24675744
[SGTA]
|
|
Oxidoreductase and Chaperone Activities Co-opted by Nonenveloped Polyomaviruses During Entry
,Walczak, CP;,
Thesis
[SGTA]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201429L1 | Lenti ORF clone of Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC201429L2 | Lenti ORF clone of Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA), mGFP tagged |
CNY 5,890.00 |
|
| RC201429L3 | Lenti ORF clone of Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201429L4 | Lenti ORF clone of Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA), mGFP tagged |
CNY 5,890.00 |
|
| RG201429 | SGTA (tGFP-tagged) - Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA) |
CNY 4,000.00 |
|
| SC118251 | SGTA (untagged)-Human small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (SGTA) |
CNY 2,400.00 |
