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FAM62A (ESYT1) (NM_015292) Human Tagged ORF Clone
CAT＃: RC201380
ESYT1 (Myc-DDK-tagged)-Human extended synaptotagmin-like protein 1 (ESYT1), transcript variant 2
ORF Plasmid: tGFP
"NM_015292" in other vectors (4)
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CNY 7,688.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FAM62A; MBC2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201380 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGATCTCCAGGAGAGGGCCCCAGCCCCAGCCCCATGGACCAGCCCTCTGCTCCCTCCGACCCCA CTGACCAGCCCCCCGCTGCTCACGCAAAGCCAGACCCAGGTTCTGGGGGCCAACCTGCTGGCCCTGGCGC GGCGGGTGAGGCCCTGGCGGTGCTGACTTCATTCGGGAGGCGGTTGCTGGTGCTGATACCTGTGTATTTG GCCGGGGCAGTGGGACTCAGCGTGGGTTTCGTGCTCTTCGGCCTCGCCCTCTACCTGGGCTGGCGCCGGG TCCGCGACGAGAAAGAACGGAGCCTTCGAGCAGCGAGGCAGCTACTGGACGACGAGGAGCAGCTCACTGC GAAAACTCTCTATATGAGTCATCGAGAGCTACCTGCCTGGGTCAGCTTCCCAGACGTGGAAAAGGCTGAA TGGCTCAATAAGATTGTGGCCCAGGTCTGGCCCTTCCTGGGCCAGTATATGGAGAAGCTTCTGGCTGAAA CTGTGGCTCCGGCTGTTAGGGGATCTAACCCCCATCTGCAAACATTTACATTTACACGAGTGGAACTGGG TGAAAAGCCATTGCGCATCATTGGAGTCAAGGTTCACCCAGGTCAGAGAAAAGAGCAGATCCTGCTGGAC TTGAACATCAGCTATGTAGGTGATGTGCAGATTGATGTGGAAGTGAAGAAATATTTTTGCAAAGCAGGAG TCAAGGGCATGCAGCTACATGGCGTTTTGCGGGTGATACTGGAGCCACTCATTGGGGACCTTCCCTTCGT GGGGGCTGTGTCAATGTTCTTCATCCGACGCCCGACCCTAGACATCAACTGGACAGGGATGACCAACCTG CTGGATATCCCAGGACTTAGCTCACTCTCTGACACCATGATCATGGACTCCATTGCTGCCTTCCTCGTGT TGCCCAACCGATTACTGGTGCCCCTTGTGCCTGACCTTCAAGATGTGGCTCAGTTGCGTTCCCCTCTGCC CAGGGGCATTATTCGAATTCACCTGCTGGCTGCTCGAGGGCTGAGTTCCAAGGACAAATATGTGAAGGGC CTGATTGAGGGCAAGTCAGACCCATATGCACTTGTGCGTTTGGGTACCCAGACATTCTGCAGTCGTGTCA TTGATGAAGAACTCAACCCACAGTGGGGAGAGACTTATGAGGTGATGGTACACGAGGTCCCAGGGCAGGA GATTGAAGTGGAGGTGTTCGACAAGGATCCAGATAAAGATGACTTTCTGGGCAGAATGAAGCTGGATGTA GGGAAGGTGTTACAGGCTAGCGTTCTGGATGATTGGTTCCCTCTACAAGGTGGGCAAGGCCAAGTTCACT TGAGGCTAGAATGGCTGTCACTTTTGTCAGATGCAGAGAAACTGGAGCAGGTTCTACAGTGGAATTGGGG AGTCTCCTCTCGACCAGATCCCCCGTCAGCTGCCATCTTAGTTGTCTACCTGGATCGGGCCCAGGATCTT CCTCTGAAGAAGGGGAACAAGGAACCCAACCCTATGGTACAACTGTCAATTCAGGATGTGACTCAGGAGA GCAAGGCTGTCTACAGTACCAACTGCCCAGTGTGGGAGGAAGCGTTCCGGTTCTTCCTACAAGACCCTCA AAGCCAGGAGCTCGATGTGCAAGTGAAGGATGATTCCAGGGCCCTGACTTTAGGAGCACTGACGCTGCCT CTGGCCCGCCTGCTGACTGCCCCAGAACTCATCCTGGACCAGTGGTTCCAGCTCAGCAGCTCTGGTCCAA ACTCCAGACTCTATATGAAACTAGTCATGAGGATCCTGTACTTGGATTCATCAGAAATATGCTTCCCCAC GGTGCCTGGTTGTCCTGGTGCTTGGGACGTGGACAGTGAGAATCCCCAGAGAGGCAGCAGTGTGGATGCC CCACCTCGACCCTGTCACACGACTCCTGATAGCCAGTTTGGGACTGAGCATGTGCTTCGGATCCATGTAT TAGAGGCCCAGGACCTGATTGCCAAAGACCGTTTCTTGGGGGGACTGGTGAAGGGCAAGTCAGACCCCTA TGTCAAACTAAAGTTGGCAGGACGAAGCTTCCGGAGCCATGTTGTTCGGGAAGATCTCAATCCCCGCTGG AATGAGGTTTTTGAGGTGATCGTCACATCAGTTCCAGGCCAAGAGCTAGAGGTTGAAGTCTTTGACAAGG ACTTGGACAAGGATGATTTTCTGGGCAGGTGTAAAGTGCGTCTCACCACAGTCTTAAACAGTGGCTTCCT TGATGAGTGGCTGACCCTGGAGGATGTCCCATCTGGCCGCCTGCACTTGCGCCTGGAGCGTCTCACCCCC CGTCCCACTGCTGCTGAGTTAGAGGAGGTGCTGCAGGTGAATAGTTTGATCCAGACTCAGAAGAGTGCGG AGCTGGCTGCGGCCCTGCTATCCATCTATATGGAGCGGGCAGAGGACCTCCCGCTGCGAAAAGGCACCAA GCACCTCAGCCCTTATGCTACTCTCACTGTGGGAGATAGTTCTCATAAAACCAAGACTATTTCGCAAACT TCAGCCCCTGTCTGGGATGAGAGTGCCTCCTTTCTCATCAGGAAACCACACACTGAGAGCCTAGAGTTGC AGGTTCGGGGTGAGGGCACTGGCGTGCTGGGCTCATTATCCCTGCCCCTCTCAGAGCTCCTCGTGGCTGA CCAGCTCTGCTTGGACCGCTGGTTTACACTCAGCAGTGGTCAGGGGCAGGTGCTACTGAGAGCACAGCTA GGGATCCTGGTGTCCCAGCACTCGGGAGTGGAAGCTCATAGCCACAGCTACAGCCACAGCTCCTCATCGC TGAGTGAAGAACCAGAGCTCTCGGGGGGACCCCCTCACATCACCTCCTCAGCCCCAGAGCTCCGGCAGCG CCTAACACATGTTGACAGTCCCCTTGAGGCTCCAGCCGGGCCTCTGGGCCAGGTGAAACTGACTCTGTGG TACTACAGTGAAGAACGAAAGCTGGTCAGCATTGTTCATGGTTGCCGGTCCCTTCGACAGAATGGACGTG ATCCTCCTGATCCCTATGTGTCACTGTTGCTACTGCCAGACAAGAACCGAGGCACCAAGAGGAGGACCTC ACAGAAGAAGAGGACCCTGAGTCCTGAATTTAATGAACGGTTTGAGTGGGAACTCCCCCTGGATGAGGCC CAGAGACGAAAGCTGGATGTCTCTGTCAAGTCTAATTCCTCCTTCATGTCAAGAGAGCGTGAGCTGCTGG GGAAGGTGCAGCTGGACCTAGCTGAGACAGACCTTTCCCAGGGTGTAGCCCGGTGGTATGACCTGATGGA CAACAAGGACAAGGGCAGCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201380 protein sequence
Red=Cloning site Green=Tags(s) MERSPGEGPSPSPMDQPSAPSDPTDQPPAAHAKPDPGSGGQPAGPGAAGEALAVLTSFGRRLLVLIPVYL AGAVGLSVGFVLFGLALYLGWRRVRDEKERSLRAARQLLDDEEQLTAKTLYMSHRELPAWVSFPDVEKAE WLNKIVAQVWPFLGQYMEKLLAETVAPAVRGSNPHLQTFTFTRVELGEKPLRIIGVKVHPGQRKEQILLD LNISYVGDVQIDVEVKKYFCKAGVKGMQLHGVLRVILEPLIGDLPFVGAVSMFFIRRPTLDINWTGMTNL LDIPGLSSLSDTMIMDSIAAFLVLPNRLLVPLVPDLQDVAQLRSPLPRGIIRIHLLAARGLSSKDKYVKG LIEGKSDPYALVRLGTQTFCSRVIDEELNPQWGETYEVMVHEVPGQEIEVEVFDKDPDKDDFLGRMKLDV GKVLQASVLDDWFPLQGGQGQVHLRLEWLSLLSDAEKLEQVLQWNWGVSSRPDPPSAAILVVYLDRAQDL PLKKGNKEPNPMVQLSIQDVTQESKAVYSTNCPVWEEAFRFFLQDPQSQELDVQVKDDSRALTLGALTLP LARLLTAPELILDQWFQLSSSGPNSRLYMKLVMRILYLDSSEICFPTVPGCPGAWDVDSENPQRGSSVDA PPRPCHTTPDSQFGTEHVLRIHVLEAQDLIAKDRFLGGLVKGKSDPYVKLKLAGRSFRSHVVREDLNPRW NEVFEVIVTSVPGQELEVEVFDKDLDKDDFLGRCKVRLTTVLNSGFLDEWLTLEDVPSGRLHLRLERLTP RPTAAELEEVLQVNSLIQTQKSAELAAALLSIYMERAEDLPLRKGTKHLSPYATLTVGDSSHKTKTISQT SAPVWDESASFLIRKPHTESLELQVRGEGTGVLGSLSLPLSELLVADQLCLDRWFTLSSGQGQVLLRAQL GILVSQHSGVEAHSHSYSHSSSSLSEEPELSGGPPHITSSAPELRQRLTHVDSPLEAPAGPLGQVKLTLW YYSEERKLVSIVHGCRSLRQNGRDPPDPYVSLLLLPDKNRGTKRRTSQKKRTLSPEFNERFEWELPLDEA QRRKLDVSVKSNSSFMSRERELLGKVQLDLAETDLSQGVARWYDLMDNKDKGSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015292 |
| ORF Size | 3312 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015292.3 |
| RefSeq Size | 4255 bp |
| RefSeq ORF | 3315 bp |
| Locus ID | 23344 |
| UniProt ID | Q9BSJ8 |
| Domains | C2 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 122.9 kDa |
| Gene Summary | Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity). Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201380L3 | Lenti ORF clone of Human extended synaptotagmin-like protein 1 (ESYT1), transcript variant 2, Myc-DDK-tagged |
CNY 11,310.00 |
|
| RC201380L4 | Lenti ORF clone of Human extended synaptotagmin-like protein 1 (ESYT1), transcript variant 2, mGFP tagged |
CNY 11,310.00 |
|
| RG201380 | ESYT1 (tGFP-tagged) - Human extended synaptotagmin-like protein 1 (ESYT1), transcript variant 2 |
CNY 9,288.00 |
|
| SC107971 | ESYT1 (untagged)-Human extended synaptotagmin-like protein 1 (ESYT1), transcript variant 2 |
CNY 7,696.00 |
