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FKBP12 (FKBP1A) (NM_000801) Human Tagged ORF Clone
CAT＃: RC201237
FKBP1A (Myc-DDK-tagged)-Human FK506 binding protein 1A, 12kDa (FKBP1A), transcript variant 1
ORF Plasmid: tGFP
"NM_000801" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FKBP-1A; FKBP-12; FKBP1; FKBP12; PKC12; PKCI2; PPIASE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201237 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGTGCAGGTGGAAACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGCCAGACCTGCG TGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAATTTGATTCCTCCCGGGACAGAAACAAGCCCTT TAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTGCCCAGATGAGTGTGGGT CAGAGAGCCAAACTGACTATATCTCCAGATTATGCCTATGGTGCCACTGGGCACCCAGGCATCATCCCAC CACATGCCACTCTCGTCTTCGATGTGGAGCTTCTAAAACTGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201237 protein sequence
Red=Cloning site Green=Tags(s) MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVG QRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000801 |
| ORF Size | 324 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000801.5 |
| RefSeq Size | 1643 bp |
| RefSeq ORF | 327 bp |
| Locus ID | 2280 |
| UniProt ID | P62942 |
| Domains | FKBP |
| Protein Families | Druggable Genome |
| MW | 12 kDa |
| Gene Summary | The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to this gene, at least one of which is transcribed. [provided by RefSeq, Sep 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Phenotypic screen identifies calcineurin-sparing FK506 analogs as BMP potentiators for treatment of acute kidney injury
,Larraufie, MH;Gao, X;Xia, X;Devine, PJ;Kallen, J;Liu, D;Michaud, G;Harsch, A;Savage, N;Ding, J;Tan, K;Mihalic, M;Roggo, S;Canham, SM;Bushell, SM;Krastel, P;Gao, J;Izaac, A;Altinoglu, E;Lustenberger, P;Salcius, M;Harbinski, F;Williams, ET;Zeng, L;Loureiro, J;Cong, F;Fryer, CJ;Klickstein, L;Tallarico, JA;Jain, RK;Rothman, DM;Wang, S;,
Cell chemical biology
,PubMed ID 33894161
[FKBP12]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201237L1 | Lenti ORF clone of Human FK506 binding protein 1A, 12kDa (FKBP1A), transcript variant 1, Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC201237L2 | Lenti ORF clone of Human FK506 binding protein 1A, 12kDa (FKBP1A), transcript variant 1, mGFP tagged |
CNY 3,600.00 |
|
| RC201237L3 | Lenti ORF clone of Human FK506 binding protein 1A, 12kDa (FKBP1A), transcript variant 1, Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC201237L4 | Lenti ORF clone of Human FK506 binding protein 1A, 12kDa (FKBP1A), transcript variant 1, mGFP tagged |
CNY 3,600.00 |
|
| RG201237 | FKBP1A (tGFP-tagged) - Human FK506 binding protein 1A, 12kDa (FKBP1A), transcript variant 1 |
CNY 2,800.00 |
|
| SC109241 | FKBP1A (untagged)-Human FK506 binding protein 1A, 12kDa (FKBP1A), transcript variant 1 |
CNY 1,800.00 |
