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ISG15 (NM_005101) Human Tagged ORF Clone
CAT＃: RC201235
- TrueORF®
ISG15 (Myc-DDK-tagged)-Human ISG15 ubiquitin-like modifier (ISG15)
ORF Plasmid: tGFP
"NM_005101" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | G1P2; hUCRP; IFI15; IMD38; IP17; UCRP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201235 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGGGACCTGACGGTGAAGATGCTGGCGGGCAACGAATTCCAGGTGTCCCTGAGCAGCTCCATGT CGGTGTCAGAGCTGAAGGCGCAGATCACCCAGAAGATCGGCGTGCACGCCTTCCAGCAGCGTCTGGCTGT CCACCCGAGCGGTGTGGCGCTGCAGGACAGGGTCCCCCTTGCCAGCCAGGGCCTGGGCCCCGGCAGCACG GTCCTGCTGGTGGTGGACAAATGCGACGAACCTCTGAACATCCTGGTGAGGAATAACAAGGGCCGCAGCA GCACCTACGAGGTGCGGCTGACGCAGACCGTGGCCCACCTGAAGCAGCAAGTGAGCGGGCTGGAGGGTGT GCAGGACGACCTGTTCTGGCTGACCTTCGAGGGGAAGCCCCTGGAGGACCAGCTCCCGCTGGGGGAGTAC GGCCTCAAGCCCCTGAGCACCGTGTTCATGAATCTGCGCCTGCGGGGAGGCGGCACAGAGCCTGGCGGGC GGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201235 protein sequence
Red=Cloning site Green=Tags(s) MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGST VLLVVDKCDEPLNILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEY GLKPLSTVFMNLRLRGGGTEPGGRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005101 |
| ORF Size | 495 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005101.4 |
| RefSeq Size | 685 bp |
| RefSeq ORF | 498 bp |
| Locus ID | 9636 |
| UniProt ID | P05161 |
| Domains | UBQ |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | RIG-I-like receptor signaling pathway |
| MW | 17.9 kDa |
| Gene Summary | The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Hepatitis C virus enhances Rubicon expression, leading to autophagy inhibition and intracellular innate immune activation
,Shiode, Y;Hikita, H;Tanaka, S;Shirai, K;Doi, A;Sakane, S;Kai, Y;Nakabori, T;Yamada, R;Kodama, T;Narumi, R;Sakamori, R;Eguchi, H;Tomonaga, T;Tatsumi, T;Takehara, T;,
Scientific reports
,PubMed ID 32943718
[ISG15]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201235L1 | Lenti ORF clone of Human ISG15 ubiquitin-like modifier (ISG15), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC201235L2 | Lenti ORF clone of Human ISG15 ubiquitin-like modifier (ISG15), mGFP tagged |
CNY 5,890.00 |
|
| RC201235L3 | Lenti ORF clone of Human ISG15 ubiquitin-like modifier (ISG15), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC201235L4 | Lenti ORF clone of Human ISG15 ubiquitin-like modifier (ISG15), mGFP tagged |
CNY 3,600.00 |
|
| RG201235 | ISG15 (tGFP-tagged) - Human ISG15 ubiquitin-like modifier (ISG15) |
CNY 2,800.00 |
|
| SC116926 | ISG15 (untagged)-Human ISG15 ubiquitin-like modifier (ISG15) |
CNY 1,200.00 |
