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Cytokeratin 15 (KRT15) (NM_002275) Human Tagged ORF Clone
CAT＃: RC201150
KRT15 (Myc-DDK-tagged)-Human keratin 15 (KRT15)
ORF Plasmid: tGFP
"NM_002275" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CK15; K1CO; K15 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201150 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACCACATTTCTGCAAACTTCTTCCTCCACCTTTGGGGGTGGCTCAACCCGAGGGGGTTCCCTCC TGGCTGGGGGAGGTGGCTTTGGTGGGGGGAGTCTCTCTGGGGGAGGTGGAAGCCGAAGTATCTCAGCTTC TTCTGCTAGGTTTGTCTCTTCAGGGTCAGGAGGAGGATATGGGGGTGGCATGAGGGTCTGTGGCTTTGGT GGAGGGGCTGGTAGTGTTTTCGGTGGAGGCTTTGGAGGGGGCGTTGGTGGGGGTTTTGGTGGTGGCTTTG GTGGTGGCGATGGTGGTCTCCTCTCTGGCAATGAGAAAATTACCATGCAGAACCTCAATGACCGCCTGGC CTCCTACCTGGACAAGGTACGTGCCCTGGAGGAGGCCAATGCTGACCTGGAGGTGAAGATCCATGACTGG TACCAGAAGCAGACCCCAACCAGCCCAGAATGCGACTACAGCCAATACTTCAAGACCATTGAAGAGCTCC GGGACAAGATCATGGCCACCACCATCGACAACTCCCGGGTCATCCTGGAGATCGACAATGCCAGGCTGGC TGCGGACGACTTCAGGCTCAAGTATGAGAATGAGCTGGCCCTGCGCCAGGGCGTTGAGGCTGACATCAAC GGCTTGCGCCGAGTCCTGGATGAGCTGACCCTGGCCAGGACTGACCTGGAGATGCAGATCGAGGGCCTGA ATGAGGAGCTAGCCTACCTGAAGAAGAACCACGAAGAGGAGATGAAGGAGTTCAGCAGCCAGCTGGCCGG CCAGGTCAATGTGGAGATGGACGCAGCACCGGGTGTGGACCTGACCCGTGTGCTGGCAGAGATGAGGGAG CAGTACGAGGCCATGGCGGAGAAGAACCGCCGGGATGTCGAGGCCTGGTTCTTCAGCAAGACTGAGGAGC TGAACAAAGAGGTGGCCTCCAACACAGAAATGATCCAGACCAGCAAGACGGAGATCACAGACCTGAGACG CACGATGCAGGAGCTGGAGATCGAGCTGCAGTCCCAGCTCAGCATGAAAGCTGGGCTGGAGAACTCACTG GCCGAGACAGAGTGCCGCTATGCCACGCAGCTGCAGCAGATCCAGGGGCTCATTGGTGGCCTGGAGGCCC AGCTGAGTGAGCTCCGATGCGAGATGGAGGCTCAGAACCAGGAGTACAAGATGCTGCTTGACATAAAGAC ACGGCTGGAGCAGGAGATCGCTACTTACCGCAGCCTGCTCGAGGGCCAGGATGCCAAGATGGCTGGCATT GGCATCAGGGAAGCCTCTTCAGGAGGTGGTGGTAGCAGCAGCAATTTCCACATCAATGTAGAAGAGTCAG TGGATGGACAGGTGGTTTCTTCCCACAAGAGAGAAATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201150 protein sequence
Red=Cloning site Green=Tags(s) MTTTFLQTSSSTFGGGSTRGGSLLAGGGGFGGGSLSGGGGSRSISASSARFVSSGSGGGYGGGMRVCGFG GGAGSVFGGGFGGGVGGGFGGGFGGGDGGLLSGNEKITMQNLNDRLASYLDKVRALEEANADLEVKIHDW YQKQTPTSPECDYSQYFKTIEELRDKIMATTIDNSRVILEIDNARLAADDFRLKYENELALRQGVEADIN GLRRVLDELTLARTDLEMQIEGLNEELAYLKKNHEEEMKEFSSQLAGQVNVEMDAAPGVDLTRVLAEMRE QYEAMAEKNRRDVEAWFFSKTEELNKEVASNTEMIQTSKTEITDLRRTMQELEIELQSQLSMKAGLENSL AETECRYATQLQQIQGLIGGLEAQLSELRCEMEAQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMAGI GIREASSGGGGSSSNFHINVEESVDGQVVSSHKREI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002275 |
| ORF Size | 1368 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002275.3 |
| RefSeq Size | 1861 bp |
| RefSeq ORF | 1371 bp |
| Locus ID | 3866 |
| UniProt ID | P19012 |
| Domains | filament |
| MW | 49.2 kDa |
| Gene Summary | The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201150L3 | Lenti ORF clone of Human keratin 15 (KRT15), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201150L4 | Lenti ORF clone of Human keratin 15 (KRT15), mGFP tagged |
CNY 5,890.00 |
|
| RG201150 | KRT15 (tGFP-tagged) - Human keratin 15 (KRT15) |
CNY 5,256.00 |
|
| SC118728 | KRT15 (untagged)-Human keratin 15 (KRT15) |
CNY 3,656.00 |
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