ASCL1 (NM_004316) Human Tagged ORF Clone
CAT#: RC201123
ASCL1 (Myc-DDK-tagged)-Human achaete-scute complex homolog 1 (Drosophila) (ASCL1)
ORF Plasmid: tGFP
"NM_004316" in other vectors (7)
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CNY 2,400.00
CNY 3,705.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ASH1; bHLHa46; HASH1; MASH1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201123 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGCTCTGCCAAGATGGAGAGCGGCGGCGCCGGCCAGCAGCCCCAGCCGCAGCCCCAGCAGCCCT TCCTGCCGCCCGCAGCCTGTTTCTTTGCCACGGCCGCAGCCGCGGCGGCCGCAGCCGCCGCAGCGGCAGC GCAGAGCGCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGCGCCGCAGCTGAGACCGGCGGCC GACGGCCAGCCCTCAGGGGGCGGTCACAAGTCAGCGCCCAAGCAAGTCAAGCGACAGCGCTCGTCTTCGC CCGAACTGATGCGCTGCAAACGCCGGCTCAACTTCAGCGGCTTTGGCTACAGCCTGCCGCAGCAGCAGCC GGCCGCCGTGGCGCGCCGCAACGAGCGCGAGCGCAACCGCGTCAAGTTGGTCAACCTGGGCTTTGCCACC CTTCGGGAGCACGTCCCCAACGGCGCGGCCAACAAGAAGATGAGTAAGGTGGAGACACTGCGCTCGGCGG TCGAGTACATCCGCGCGCTGCAGCAGCTGCTGGACGAGCATGACGCGGTGAGCGCCGCCTTCCAGGCAGG CGTCCTGTCGCCCACCATCTCCCCCAACTACTCCAACGACTTGAACTCCATGGCCGGCTCGCCGGTCTCA TCCTACTCGTCGGACGAGGGCTCTTACGACCCGCTCAGCCCCGAGGAGCAGGAGCTTCTCGACTTCACCA ACTGGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201123 protein sequence
Red=Cloning site Green=Tags(s) MESSAKMESGGAGQQPQPQPQQPFLPPAACFFATAAAAAAAAAAAAAQSAQQQQQQQQQQQQAPQLRPAA DGQPSGGGHKSAPKQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPAAVARRNERERNRVKLVNLGFAT LREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAGSPVS SYSSDEGSYDPLSPEEQELLDFTNWF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_004316 |
ORF Size | 708 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004316.4 |
RefSeq Size | 2490 bp |
RefSeq ORF | 711 bp |
Locus ID | 429 |
UniProt ID | P50553 |
Domains | HLH |
Protein Families | Druggable Genome, Transcription Factors |
MW | 25.5 kDa |
Gene Summary | This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
Genetic targeting of neurogenic precursors in the adult forebrain ventricular epithelium
,null,
Life Science Alliance
,PubMed ID 32482782
[ASCL1]
|
Significance of achaete-scute complex homologue 1 (ASCL1) in pulmonary neuroendocrine carcinomas; RNA sequence analyses using small cell lung cancer cells and Ascl1-induced pulmonary neuroendocrine carcinoma cells
,Kudoh, S;Tenjin, Y;Kameyama, H;Ichimura, T;Yamada, T;Matsuo, A;Kudo, N;Sato, Y;Ito, T;,
Histochem. Cell Biol.
,PubMed ID 32170367
[ASCL1]
|
Combinatorial programming of human neuronal progenitors using magnetically-guided stoichiometric mRNA delivery
,Azimi, SM;Sheridan, SD;Ghannad-Rezaie, M;Eimon, PM;Yanik, MF;,
Elife
,PubMed ID 29714688
[ASCL1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201123L1 | Lenti ORF clone of Human achaete-scute complex homolog 1 (Drosophila) (ASCL1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC201123L2 | Lenti ORF clone of Human achaete-scute complex homolog 1 (Drosophila) (ASCL1), mGFP tagged |
CNY 4,800.00 |
|
RC201123L3 | Lenti ORF clone of Human achaete-scute complex homolog 1 (Drosophila) (ASCL1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC201123L4 | Lenti ORF clone of Human achaete-scute complex homolog 1 (Drosophila) (ASCL1), mGFP tagged |
CNY 4,800.00 |
|
RG201123 | ASCL1 (tGFP-tagged) - Human achaete-scute complex homolog 1 (Drosophila) (ASCL1) |
CNY 4,000.00 |
|
SC117427 | ASCL1 (untagged)-Human achaete-scute complex homolog 1 (Drosophila) (ASCL1) |
CNY 2,400.00 |
|
SC324383 | ASCL1 (untagged)-Human achaete-scute complex homolog 1 (Drosophila) (ASCL1) |
CNY 2,400.00 |