ARPC1B (NM_005720) Human Tagged ORF Clone
CAT#: RC201103
ARPC1B (Myc-DDK-tagged)-Human actin related protein 2/3 complex, subunit 1B, 41kDa (ARPC1B)
ORF Plasmid: tGFP
"NM_005720" in other vectors (4)
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CNY 5,488.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ARC41; IMD71; p40-ARC; p41-ARC; PLTEID |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201103 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTACCACAGCTTCCTGGTGGAGCCCATCAGCTGCCACGCCTGGAACAAGGACCGCACCCAGATTG CCATCTGCCCCAACAACCATGAGGTGCATATCTATGAAAAGAGCGGTGCCAAATGGACCAAGGTGCACGA GCTCAAGGAGCACAACGGGCAGGTGACAGGCATCGACTGGGCCCCCGAGAGTAACCGTATTGTGACCTGC GGCACAGACCGCAACGCCTACGTGTGGACGCTGAAGGGCCGCACATGGAAGCCCACGCTGGTCATCCTGC GGATCAACCGGGCTGCCCGCTGCGTGCGCTGGGCCCCCAACGAGAACAAGTTTGCTGTGGGCAGCGGCTC TCGTGTGATCTCCATCTGTTATTTCGAGCAGGAGAATGACTGGTGGGTTTGCAAGCACATCAAGAAGCCC ATCCGCTCCACCGTCCTCAGCCTGGACTGGCACCCCAACAATGTGCTGCTGGCTGCCGGCTCCTGTGACT TCAAGTGTCGGATCTTTTCAGCCTACATCAAGGAGGTGGAGGAACGGCCGGCACCCACCCCGTGGGGCTC CAAGATGCCCTTTGGGGAACTGATGTTCGAATCCAGCAGTAGCTGCGGCTGGGTACATGGCGTCTGTTTC TCAGCCAGCGGGAGCCGCGTGGCCTGGGTAAGCCACGACAGCACCGTCTGCCTGGCTGATGCCGACAAGA AGATGGCCGTCGCGACTCTGGCCTCTGAAACACTACCACTGCTGGCGCTGACCTTCATCACAGACAACAG CCTGGTGGCAGCGGGCCACGACTGCTTCCCGGTGCTGTTCACCTATGACGCCGCCGCGGGGATGCTGAGC TTCGGCGGGCGGCTGGACGTTCCTAAGCAGAGCTCGCAGCGTGGCTTGACGGCCCGCGAGCGCTTCCAGA ACCTGGACAAGAAGGCGAGCTCCGAGGGTGGCACGGCTGCGGGCGCGGGCCTAGACTCGCTGCACAAGAA CAGCGTCAGCCAGATCTCGGTGCTCAGCGGCGGCAAGGCCAAGTGCTCGCAGTTCTGCACCACTGGCATG GATGGCGGCATGAGTATCTGGGATGTGAAGAGCTTGGAGTCAGCCTTGAAGGACCTCAAGATCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201103 protein sequence
Red=Cloning site Green=Tags(s) MAYHSFLVEPISCHAWNKDRTQIAICPNNHEVHIYEKSGAKWTKVHELKEHNGQVTGIDWAPESNRIVTC GTDRNAYVWTLKGRTWKPTLVILRINRAARCVRWAPNENKFAVGSGSRVISICYFEQENDWWVCKHIKKP IRSTVLSLDWHPNNVLLAAGSCDFKCRIFSAYIKEVEERPAPTPWGSKMPFGELMFESSSSCGWVHGVCF SASGSRVAWVSHDSTVCLADADKKMAVATLASETLPLLALTFITDNSLVAAGHDCFPVLFTYDAAAGMLS FGGRLDVPKQSSQRGLTARERFQNLDKKASSEGGTAAGAGLDSLHKNSVSQISVLSGGKAKCSQFCTTGM DGGMSIWDVKSLESALKDLKIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_005720 |
ORF Size | 1116 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005720.4 |
RefSeq Size | 1551 bp |
RefSeq ORF | 1119 bp |
Locus ID | 10095 |
UniProt ID | O15143 |
Domains | WD40 |
Protein Pathways | Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton |
MW | 40.9 kDa |
Gene Summary | This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. This protein also has a role in centrosomal homeostasis by being an activator and substrate of the Aurora A kinase. [provided by RefSeq, Mar 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201103L3 | Lenti ORF clone of Human actin related protein 2/3 complex, subunit 1B, 41kDa (ARPC1B), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201103L4 | Lenti ORF clone of Human actin related protein 2/3 complex, subunit 1B, 41kDa (ARPC1B), mGFP tagged |
CNY 5,890.00 |
|
RG201103 | ARPC1B (tGFP-tagged) - Human actin related protein 2/3 complex, subunit 1B, 41kDa (ARPC1B) |
CNY 7,088.00 |
|
SC126722 | ARPC1B (untagged)-Human actin related protein 2/3 complex, subunit 1B, 41kDa (ARPC1B) |
CNY 3,656.00 |