TOMM34 (NM_006809) Human Tagged ORF Clone

CAT#: RC201083

TOMM34 (Myc-DDK-tagged)-Human translocase of outer mitochondrial membrane 34 (TOMM34), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP



  "NM_006809" in other vectors (6)

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CNY 2,400.00

CNY 4,465.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


TOMM34 mouse monoclonal antibody, clone OTI2A9 (formerly 2A9)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HTOM34P; TOM34; URCC3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201083 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCCAAATTCCCAGACTCTGTGGAGGAGCTCCGCGCCGCCGGCAATGAGAGTTTCCGCAACGGCC
AGTACGCCGAGGCCTCCGCGCTCTACGGCCGCGCGCTGCGGGTGCTGCAGGCGCAAGGTTCTTCAGACCC
AGAAGAAGAAAGTGTTCTCTACTCCAACCGAGCAGCATGTCACTTGAAGGATGGAAACTGCAGAGACTGC
ATCAAAGATTGCACTTCAGCACTGGCCTTGGTTCCCTTCAGCATTAAGCCCCTGCTGCGGCGAGCATCTG
CTTATGAGGCTCTGGAGAAGTACCCTATGGCCTATGTTGACTATAAGACTGTGCTGCAGATTGATGATAA
TGTGACGTCAGCCGTAGAAGGCATCAACAGAATGACCAGAGCTCTCATGGACTCGCTTGGGCCTGAGTGG
CGCCTGAAGCTGCCCTCAATCCCCTTGGTGCCTGTTTCAGCTCAGAAGAGGTGGAATTCCTTGCCTTCGG
AGAACCACAAAGAGATGGCTAAAAGCAAATCCAAAGAAACCACAGCTACAAAGAACAGAGTGCCTTCTGC
TGGGGATGTGGAGAAAGCCAGAGTTCTGAAGGAAGAAGGCAATGAGCTTGTAAAGAAGGGAAACCATAAG
AAAGCTATTGAGAAGTACAGTGAAAGCCTCTTGTGTAGTAACCTGGAATCTGCCACGTACAGCAACAGAG
CACTCTGCTATTTGGTCCTGAAGCAGTACACAGAAGCAGTGAAGGACTGCACAGAAGCCCTCAAGCTGGA
TGGAAAGAACGTGAAGGCATTCTACAGACGGGCTCAAGCCCACAAAGCACTCAAGGACTATAAATCCAGC
TTTGCAGACATCAGCAACCTCCTACAGATTGAGCCTAGGAATGGTCCTGCACAGAAGTTGCGGCAGGAAG
TGAAGCAGAACCTACAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201083 protein sequence
Red=Cloning site Green=Tags(s)

MAPKFPDSVEELRAAGNESFRNGQYAEASALYGRALRVLQAQGSSDPEEESVLYSNRAACHLKDGNCRDC
IKDCTSALALVPFSIKPLLRRASAYEALEKYPMAYVDYKTVLQIDDNVTSAVEGINRMTRALMDSLGPEW
RLKLPSIPLVPVSAQKRWNSLPSENHKEMAKSKSKETTATKNRVPSAGDVEKARVLKEEGNELVKKGNHK
KAIEKYSESLLCSNLESATYSNRALCYLVLKQYTEAVKDCTEALKLDGKNVKAFYRRAQAHKALKDYKSS
FADISNLLQIEPRNGPAQKLRQEVKQNLH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006809
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006809.5
RefSeq Size 2066 bp
RefSeq ORF 930 bp
Locus ID 10953
UniProt ID Q15785
Domains TPR
Protein Families Druggable Genome
MW 34.6 kDa
Gene Summary The protein encoded by this gene is involved in the import of precursor proteins into mitochondria. The encoded protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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