Mutarotase (GALM) (NM_138801) Human Tagged ORF Clone

CAT#: RC200995

GALM (Myc-DDK-tagged)-Human galactose mutarotase (aldose 1-epimerase) (GALM)

ORF Plasmid: DDK tGFP



  "NM_138801" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


GALM mouse monoclonal antibody,clone OTI4F4
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BLOCK25; GALAC4; GLAT; HEL-S-63p; IBD1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200995 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCGGTGACCAGGGCCGTGTTTGGAGAGCTGCCCTCGGGAGGAGGGACAGTGGAGAAGTTCCAGC
TGCAGTCAGACCTCTTGAGAGTGGACATCATCTCCTGGGGCTGCACGATCACAGCCCTAGAGGTCAAAGA
CAGGCAGGGGAGAGCCTCGGACGTGGTGCTTGGCTTCGCCGAGTTGGAAGGATACCTCCAAAAGCAGCCA
TACTTTGGAGCAGTTATTGGGAGGGTGGCCAACCGAATCGCCAAAGGAACCTTCAAGGTGGATGGGAAGG
AGTATCACCTGGCCATTAACAAGGAACCCAACAGTCTGCATGGAGGAGTCAGAGGGTTTGATAAAGTGCT
CTGGACCCCTCGGGTGCTGTCAAATGGCGTCCAGTTCTCGCGCATCAGTCCAGATGGTGAAGAAGGCTAC
CCCGGAGAGTTAAAAGTCTGGGTGACATACACCCTGGATGGCGGAGAGCTCATAGTCAACTACAGAGCAC
AAGCCAGTCAGGCCACACCAGTCAACCTGACCAACCATTCTTACTTCAACCTGGCAGGCCAGGCTTCCCC
AAATATAAATGACCATGAAGTCACCATAGAAGCGGATACTTATTTGCCTGTGGATGAAACCCTGATTCCT
ACAGGAGAAGTTGCCCCAGTGCAAGGCACTGCATTCGACCTGAGAAAGCCAGTGGAGCTTGGAAAACACC
TGCAGGACTTCCATCTCAATGGTTTTGACCACAATTTCTGTCTGAAGGGATCTAAAGAAAAGCATTTTTG
TGCAAGGGTGCATCATGCTGCAAGCGGGCGGGTACTAGAAGTATACACCACCCAGCCCGGGGTCCAGTTT
TACACGGGCAACTTCCTGGATGGCACATTAAAGGGCAAGAATGGAGCTGTCTATCCCAAGCACTCCGGTT
TCTGCCTGGAGACTCAGAACTGGCCTGATGCAGTCAATCAGCCCCGCTTCCCTCCTGTGCTGCTGAGGCC
TGGTGAGGAGTATGACCACACCACCTGGTTCAAGTTTTCTGTGGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200995 protein sequence
Red=Cloning site Green=Tags(s)

MASVTRAVFGELPSGGGTVEKFQLQSDLLRVDIISWGCTITALEVKDRQGRASDVVLGFAELEGYLQKQP
YFGAVIGRVANRIAKGTFKVDGKEYHLAINKEPNSLHGGVRGFDKVLWTPRVLSNGVQFSRISPDGEEGY
PGELKVWVTYTLDGGELIVNYRAQASQATPVNLTNHSYFNLAGQASPNINDHEVTIEADTYLPVDETLIP
TGEVAPVQGTAFDLRKPVELGKHLQDFHLNGFDHNFCLKGSKEKHFCARVHHAASGRVLEVYTTQPGVQF
YTGNFLDGTLKGKNGAVYPKHSGFCLETQNWPDAVNQPRFPPVLLRPGEEYDHTTWFKFSVA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_138801
ORF Size 1026 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_138801.3
RefSeq Size 2483 bp
RefSeq ORF 1029 bp
Locus ID 130589
UniProt ID Q96C23
Domains Aldose_epim
Protein Pathways Glycolysis / Gluconeogenesis
MW 37.8 kDa
Gene Summary This gene encodes an enzyme that catalyzes the epimerization of hexose sugars such as glucose and galactose. The encoded protein is expressed in the cytoplasm and has a preference for galactose. The encoded protein may be required for normal galactose metabolism by maintaining the equilibrium of alpha and beta anomers of galactose.[provided by RefSeq, Mar 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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