PSMC2 (NM_002803) Human Tagged ORF Clone
CAT#: RC200945
PSMC2 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 2 (PSMC2), transcript variant 1
ORF Plasmid: tGFP
"NM_002803" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MSS1; Nbla10058; RPT1; S7 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200945 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGATTACCTCGGTGCCGATCAGCGGAAGACCAAAGAGGATGAGAAGGACGACAAGCCCATCCGAG CTCTGGATGAGGGGGATATTGCCTTGTTGAAAACTTATGGTCAGAGCACTTACTCTAGGCAGATCAAGCA AGTTGAAGATGACATTCAGCAACTTCTCAAGAAAATTAATGAGCTCACTGGTATTAAAGAATCTGACACT GGCCTGGCCCCACCAGCACTCTGGGATTTGGCTGCAGATAAGCAGACACTCCAGAGTGAACAGCCTTTAC AGGTTGCCAGGTGTACAAAGATAATCAATGCTGATTCGGAGGACCCAAAATACATTATCAACGTAAAGCA GTTTGCCAAGTTTGTGGTGGACCTTAGTGATCAGGTGGCACCTACTGACATTGAAGAAGGGATGAGAGTG GGCGTGGATAGAAATAAATATCAAATTCACATTCCATTGCCTCCTAAGATTGACCCAACAGTTACCATGA TGCAGGTGGAAGAGAAACCTGATGTCACATACAGTGATGTTGGTGGCTGTAAGGAACAGATTGAGAAACT GCGAGAAGTAGTTGAAACCCCATTACTTCATCCAGAGAGGTTTGTGAACCTTGGCATTGAGCCTCCCAAG GGCGTGCTGCTCTTTGGTCCACCCGGTACAGGCAAGACACTCTGTGCGCGGGCAGTTGCTAATCGGACTG ATGCGTGCTTCATTCGAGTTATTGGATCTGAGCTTGTACAGAAATACGTCGGTGAGGGGGCTCGAATGGT TCGTGAACTCTTTGAAATGGCCAGAACAAAAAAAGCCTGCCTTATCTTCTTTGATGAAATTGATGCTATT GGAGGGGCTCGTTTTGATGATGGTGCTGGAGGTGACAATGAAGTGCAGAGAACAATGTTGGAACTGATCA ATCAGCTTGATGGTTTTGATCCTCGAGGCAATATTAAAGTGCTGATGGCCACTAACAGACCTGATACTTT GGATCCAGCACTGATGAGGCCAGGGAGATTGGATAGAAAAATTGAATTTAGCTTGCCCGATCTAGAGGGT CGGACCCACATATTTAAGATTCACGCTCGTTCAATGAGTGTTGAAAGAGATATCAGATTTGAACTGTTAG CACGACTGTGTCCAAATAGCACTGGTGCTGAGATTAGAAGCGTCTGCACAGAGGCTGGTATGTTTGCCAT CAGAGCACGGCGAAAAATTGCTACCGAGAAGGATTTCTTGGAAGCTGTAAATAAGGTCATTAAGTCTTAT GCCAAATTCAGTGCTACTCCTCGTTACATGACATACAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200945 protein sequence
Red=Cloning site Green=Tags(s) MPDYLGADQRKTKEDEKDDKPIRALDEGDIALLKTYGQSTYSRQIKQVEDDIQQLLKKINELTGIKESDT GLAPPALWDLAADKQTLQSEQPLQVARCTKIINADSEDPKYIINVKQFAKFVVDLSDQVAPTDIEEGMRV GVDRNKYQIHIPLPPKIDPTVTMMQVEEKPDVTYSDVGGCKEQIEKLREVVETPLLHPERFVNLGIEPPK GVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMARTKKACLIFFDEIDAI GGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEG RTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEAGMFAIRARRKIATEKDFLEAVNKVIKSY AKFSATPRYMTYN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002803 |
ORF Size | 1299 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002803.3 |
RefSeq Size | 2846 bp |
RefSeq ORF | 1302 bp |
Locus ID | 5701 |
UniProt ID | P35998 |
Domains | AAA, AAA |
Protein Pathways | Proteasome |
MW | 48.6 kDa |
Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2011] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/β-catenin pathway by inhibiting the proteasome
,Yi, JJ;Paranjape, SR;Walker, MP;Choudhury, R;Wolter, JM;Fragola, G;Emanuele, MJ;Major, MB;Zylka, MJ;,
J. Biol. Chem.
,PubMed ID 28559284
[PSMC2]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200945L1 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 2 (PSMC2), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC200945L2 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 2 (PSMC2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC200945L3 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 2 (PSMC2), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC200945L4 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 2 (PSMC2), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
RG200945 | PSMC2 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, ATPase, 2 (PSMC2), transcript variant 1 |
CNY 5,256.00 |
|
SC321353 | PSMC2 (untagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 2 (PSMC2), transcript variant 1 |
CNY 3,656.00 |